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  • MRS Proceedings, Volume 1272
  • January 2010, 1272-NN05-08

Using Peptide Hetero-assembly to Trigger Physical Gelation and Cell Encapsulation

  • Andreina Parisi-Amon (a1), Cheryl Wong Po Foo (a2), Ji Seok Lee (a3), Widya Mulyasasmita (a4) and Sarah Heilshorn (a5)
  • DOI: http://dx.doi.org/10.1557/PROC-1272-NN05-08
  • Published online: 01 February 2011
Abstract
Abstract

Stem cell transplantation holds tremendous potential for the treatment of various trauma and diseases. However, the therapeutic efficacy is often limited by poor and unpredictable post-transplantation cell survival. While hydrogels are thought to be ideal scaffolds, the sol-gel phase transitions required for cell encapsulation within commercially available biomatrices such as collagen and Matrigel often rely on non-physiological environmental triggers (e.g., pH and temperature shifts), which are detrimental to cells. To address this limitation, we have designed a novel class of protein biomaterials: Mixing-Induced Two-Component Hydrogels (MITCH) that are recombinantly engineered to undergo gelation by hetero-assembly upon mixing at constant physiological conditions, thereby enabling simple, biocompatible cell encapsulation and transplantation protocols. Building upon bio-mimicry and precise molecular-level design principles, the resulting hydrogels have tunable viscoelasticity consistent with simple polymer physics considerations. MITCH are reproducible across cell-culture systems, supporting growth of human endothelial cells, rat mesenchymal stem cells, rat neural stem cells, and human adipose-derived stem cells. Additionally, MITCH promote the differentiation of neural progenitors into neuronal phenotypes, which adopt a 3D-branched morphology within the hydrogels.

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1SM. Willerth , SE Sakiyama-Elbert , Adv. Drug Delivery Rev Rev. 60, 263 (2008).

3C. Siatskas , CC. Bernand , Current Molecular Medicine 9, 992 (2009).

4JH. Kordower , et al, Mov. Disord Disord. 13, 383 (1998).

5JH. Kordower , et a., N. Engl. J. Med. 332, 1118 (995).

6F. Cao , et al, J. Tissue Eng. Regen. Med Med. 1, 465 (2007).

7MA. Laflamme , et al, Nat. Biotechnol Biotechnol. 25, 1015 (2007).

8F. Brandl , F. Sommer , A. Goepferich , Biomaterials 28, 134 (2007).

10RM. Capito , et al, Science 319, 1812 (2008).

11L. Haines -Buterick , et al, Poc. Natl. Acad. Sci. USA 104, 7791 (2007).

12DJ. Pochan , et al, J. Am. Chem. Soc. 125, 11802 (2003).

13KL Niece , et al, J. Am. Chem. Soc. 125, 7146 (2003).

14BM. Gillette , et al, Nat. Mater. 7, 636 (2008).

15S. Wang , et al, Tissue Eng. Part A 14, 227 (2008).

16AI. Teixeira , JK. Duckworth , O. Hermanson , Cell Res. 17, 56 (2007).

17CTS. Wong Po Foo , et al, Poc. Natl. Acad. Sci. USA 106, 22067 (2009).

18JC. Crocker , DG Grier , J. C Colloid Interface Sci. 179, 298 (1996).

19X. Huang , Nat. Struct Biol. 7, 634 (2000).

20PJ. Flory , J. Am. Chem. Soc. 63, 3083 (1941).

21V. KAnelis , D. Rotin , JD. Forman-Kay , Nat. Struct. Biol. 8, 407 (2001).

22WP. Russ , et al, Nature 437, 579 (2005).

23MH. Zaman , et al, Poc. Natl. Acad. Sci. USA 103, 10889 (2006).

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