Skip to main content
×
Home
    • Aa
    • Aa

Using Peptide Hetero-assembly to Trigger Physical Gelation and Cell Encapsulation

  • Andreina Parisi-Amon (a1), Cheryl Wong Po Foo (a2), Ji Seok Lee (a3), Widya Mulyasasmita (a4) and Sarah Heilshorn (a5)...
Abstract
Abstract

Stem cell transplantation holds tremendous potential for the treatment of various trauma and diseases. However, the therapeutic efficacy is often limited by poor and unpredictable post-transplantation cell survival. While hydrogels are thought to be ideal scaffolds, the sol-gel phase transitions required for cell encapsulation within commercially available biomatrices such as collagen and Matrigel often rely on non-physiological environmental triggers (e.g., pH and temperature shifts), which are detrimental to cells. To address this limitation, we have designed a novel class of protein biomaterials: Mixing-Induced Two-Component Hydrogels (MITCH) that are recombinantly engineered to undergo gelation by hetero-assembly upon mixing at constant physiological conditions, thereby enabling simple, biocompatible cell encapsulation and transplantation protocols. Building upon bio-mimicry and precise molecular-level design principles, the resulting hydrogels have tunable viscoelasticity consistent with simple polymer physics considerations. MITCH are reproducible across cell-culture systems, supporting growth of human endothelial cells, rat mesenchymal stem cells, rat neural stem cells, and human adipose-derived stem cells. Additionally, MITCH promote the differentiation of neural progenitors into neuronal phenotypes, which adopt a 3D-branched morphology within the hydrogels.

Copyright
References
Hide All
1Willerth SM., Sakiyama-Elbert SE, Adv. Drug Delivery Rev Rev. 60, 263 (2008).
2Bjorklund A., et al, Brain Res Res. 886, 82 (2000).
3Siatskas C., Bernand CC., Current Molecular Medicine 9, 992 (2009).
4Kordower JH., et al, Mov. Disord Disord. 13, 383 (1998).
5Kordower JH., et a., N. Engl. J. Med. 332, 1118 (995).
6Cao F., et al, J. Tissue Eng. Regen. Med Med. 1, 465 (2007).
7Laflamme MA., et al, Nat. Biotechnol Biotechnol. 25, 1015 (2007).
8Brandl F., Sommer F., Goepferich A., Biomaterials 28, 134 (2007).
9Petka WA., et al, Sci Science 281, 389 (1998).
10Capito RM., et al, Science 319, 1812 (2008).
11-Buterick L. Haines, et al, Poc. Natl. Acad. Sci. USA 104, 7791 (2007).
12Pochan DJ., et al, J. Am. Chem. Soc. 125, 11802 (2003).
13Niece KL, et al, J. Am. Chem. Soc. 125, 7146 (2003).
14Gillette BM., et al, Nat. Mater. 7, 636 (2008).
15Wang S., et al, Tissue Eng. Part A 14, 227 (2008).
16Teixeira AI., Duckworth JK., Hermanson O., Cell Res. 17, 56 (2007).
17Foo CTS. Wong Po, et al, Poc. Natl. Acad. Sci. USA 106, 22067 (2009).
18Crocker JC., Grier DG, J. C Colloid Interface Sci. 179, 298 (1996).
19Huang X., Nat. Struct Biol. 7, 634 (2000).
20Flory PJ., J. Am. Chem. Soc. 63, 3083 (1941).
21KAnelis V., Rotin D., Forman-Kay JD., Nat. Struct. Biol. 8, 407 (2001).
22Russ WP., et al, Nature 437, 579 (2005).
23Zaman MH., et al, Poc. Natl. Acad. Sci. USA 103, 10889 (2006).
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 2 *
Loading metrics...

Abstract views

Total abstract views: 61 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 22nd October 2017. This data will be updated every 24 hours.