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ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees

Published online by Cambridge University Press:  20 May 2003

Ajay K. PANDEY ,
M. Sudhakara REDDY  and
Trichur S. SURYANARAYANAN
Ajay K. PANDEY
Affiliation:
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147 001, India.
M. Sudhakara REDDY
Affiliation:
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147 001, India.
Trichur S. SURYANARAYANAN
Affiliation:
Post graduate and Research Department of Botany, Ramakrishna Mission, Vivekananda College, Chennai 600 004, India. E-mail: vasu70@yahoo.com
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Abstract

Different isolates of a foliar endophytic species of Phyllosticta were isolated from different tropical tree species in India to examine genetic variation among the isolates. Internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis did not detect any variation among the isolates, suggesting that they all belong to the same species. Sequence data of the ITS region of ribosomal DNA (ITS1 and ITS2, including 5.8S rDNA) supported the identity of the present fungal isolates as P. capitalensis. These results show that P. capitalensis (teleomorph Guignardia endophyllicola?) is an ubiquitous foliar endophyte that can infect tree hosts from different families and habitats.

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Type
Research Article
Information
Mycological Research , Volume 107 , Issue 4 , April 2003 , pp. 439 - 444
Copyright
© The British Mycological Society 2003

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