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Electrophoretic karyotypes of the phytopathogenic Pyrenophora graminea and P. teres

Published online by Cambridge University Press:  01 July 2000

Maria ARAGONA ,
Monica MONTIGIANI  and
Angelo PORTA-PUGLIA
Maria ARAGONA
Affiliation:
Istituto Sperimentale per la Patologia Vegetale, Via C. G. Bertero 22, I-00156 Roma, Italy. E-mail: research@ispave.rm.it
Monica MONTIGIANI
Affiliation:
Istituto Sperimentale per la Patologia Vegetale, Via C. G. Bertero 22, I-00156 Roma, Italy. E-mail: research@ispave.rm.it
Angelo PORTA-PUGLIA
Affiliation:
Istituto Sperimentale per la Patologia Vegetale, Via C. G. Bertero 22, I-00156 Roma, Italy. E-mail: research@ispave.rm.it
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Abstract

Contour-clamped homogeneous electric field has been applied to analyse genome size and organisation of two important pathogens of barley, Pyrenophora graminea and P. teres.

Electrophoretic karyotypes of four P. graminea isolates resolved 6–8 chromosomal bands, depending on the isolate, while the one P. teres isolate analysed contained six chromosomal bands. Chromosome length polymorphism was observed among P. graminea isolates. For both fungi the chromosomes ranged from 1.6 to more than 6 Mb, with chromosomes larger than 6 Mb migrating as a single band within the compression zone of the gel.

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Type
Research Article
Information
Mycological Research , Volume 104 , Issue 7 , July 2000 , pp. 853 - 857
Copyright
© The British Mycological Society 2000

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