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Ribosomal internal transcribed spacer size variation correlated with RAPD-PCR pattern polymorphisms in the entomopathogenic fungus Erynia neoaphidis and some closely related species

Published online by Cambridge University Press:  01 May 1997

E. ROHEL ,
Y. COUTEAUDIER ,
B. PAPIEROK ,
N. CAVELIER  and
C. A. DEDRYVER
E. ROHEL
Affiliation:
Present address: IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS18 9AF, U.K. Laboratoire de Zoologie, INRA, Domaine de la Motte, 35653 Le Rheu Cedex, France
Y. COUTEAUDIER
Affiliation:
Station de Recherche en Lutte Biologique, INRA, La Minière, 78285 Guyancourt, France
B. PAPIEROK
Affiliation:
Unité d'écologie des systèmes vectoriels, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris Cedex 15, France
N. CAVELIER
Affiliation:
Unité de Recherches Intégrées en Grandes Cultures, INRA, Domaine de la Motte, 35653 Le Rheu Cedex, France
C. A. DEDRYVER
Affiliation:
Laboratoire de Zoologie, INRA, Domaine de la Motte, 35653 Le Rheu Cedex, France
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Abstract

Erynia neoaphidis is the most widespread aphid pathogen in temperate regions. A few authors have identified this species as infecting some non-aphid hosts. It exhibits high variability with respect to morphology and cultural characteristics that underlines the need for assessment of the genetic variability within this species. We used size of PCR-amplified ribosomal DNA internal transcribed spacer (ITS) coupled with RAPD-PCR pattern in order to investigate genetic variability within a sample of 30 isolates identified as E. neoaphidis and one isolate of E. kondoiensis from diverse countries and hosts. Isolates fell into four discrete ITS size groups: 575 base pairs (bp) in isolates from non-aphid hosts, 1000 (including the E. kondoiensis isolate), 1100, or 1450 bp in those from Aphididae. The 1100-type seems to be the one of most widespread, since two-thirds of the isolates from diverse locations belong to this type. ITS types can be correlated with morphological and physiological characteristics. A great deal of genetic variability exists within ITS types, as shown by RAPD-PCR. RAPD groupings could be related with geographical origin in some cases. There was no apparent relationship between host and ITS type or RAPD pattern among isolates from Aphididae. PCR-based technologies provide evidence that could be used to clarify the taxonomy of E. neoaphidis and closely related species, and will help identify markers related to epidemiological characteristics.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 5 , May 1997 , pp. 573 - 579
Copyright
The British Mycological Society 1997

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