Identification of four starch-branching enzymes in barley endosperm: partial purification of forms I, IIa and IIb

CHUANXIN SUN; P. SATHISH; STAFFAN AHLANDSBERG; ANNA DEIBER; CHRISTER JANSSON

doi:10.1046/j.1469-8137.1997.00796.x

Abstract

Developing caryopses of barley (Hordeum vulgare L. cv. Golf) were monitored for starch-branching enzyme activity for 1 month after anthesis. Homogenized caryopses from grains with the highest specific activity were used as starting material for purification of branching enzyme by FPLC chromatography. Several branching enzyme activity fractions were resolved. We have previously described the isolation of a 51/50 kDa protein with starch branching activity from one of these fractions. From three other fractions, we have now partially purified starch branching enzymes corresponding to forms I, IIa and IIb.

Information

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Sun, Chuanxin Sathish, Puthigae Ahlandsberg, Staffan and Jansson, Christer 1998. The Two Genes Encoding Starch-Branching Enzymes IIa and IIb Are Differentially Expressed in Barley1. Plant Physiology, Vol. 118, Issue. 1, p. 37.

Sun, Chuanxin Sathish, P. Ahlandsberg, Staffan and Jansson, Christer 1999. Analyses of isoamylase gene activity in wild-type barley indicate its involvement in starch synthesis. Plant Molecular Biology, Vol. 40, Issue. 3, p. 431.

Schulman, Alan H. Runeberg-Roos, Pia and Jääskeläinen, Marko 2000. Carbohydrate Reserves in Plants - Synthesis and Regulation. Vol. 26, Issue. , p. 147.

Repellin, Anne Båga, Monica and Chibbar, Ravindra N. 2001. Characterization of a cDNA encoding a type I starch branching enzyme produced in developing wheat (Triticum aestivum L.)kernels. Journal of Plant Physiology, Vol. 158, Issue. 1, p. 91.

Shewry, Peter R and Morell, Matthew 2001. Advances in Botanical Research Volume 34. Vol. 34, Issue. , p. 165.

Nishi, Aiko Nakamura, Yasunori Tanaka, Naoki and Satoh, Hikaru 2001. Biochemical and Genetic Analysis of the Effects ofAmylose-Extender Mutation in Rice Endosperm. Plant Physiology, Vol. 127, Issue. 2, p. 459.

Rahman, Sadequr Regina, Ahmed Li, Zhongyi Mukai, Yasuhiko Yamamoto, Maki Kosar-Hashemi, Behjat Abrahams, Sharon and Morell, Matthew K. 2001. Comparison of Starch-Branching Enzyme Genes Reveals Evolutionary Relationships Among Isoforms. Characterization of a Gene for Starch-Branching Enzyme IIa from the Wheat D Genome DonorAegilops tauschii . Plant Physiology, Vol. 125, Issue. 3, p. 1314.

Gao, Ming Båga, Monica and Chibbar, Ravindra 2002. Transgenic Plants and Crops.

Yoo, Sang-Ho Spalding, Martin H and Jane, Jay-lin 2002. Characterization of cyanobacterial glycogen isolated from the wild type and from a mutant lacking of branching enzyme. Carbohydrate Research, Vol. 337, Issue. 21-23, p. 2195.

Schulman, AIan 2002. Plant Biotechnology and Transgenic Plants.

Morell, Matthew K. Kosar‐Hashemi, Behjat Cmiel, Mark Samuel, Michael S. Chandler, Peter Rahman, Sadequr Buleon, Alain Batey, Ian L. and Li, Zhongyi 2003. Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel properties. The Plant Journal, Vol. 34, Issue. 2, p. 173.

Mutisya, Joel Sathish, P. Sun, Chuanxin Andersson, Lena Ahlandsberg, Staffan Baguma, Yona Palmqvist, Sara Odhiambo, Benjamin Åman, P.e.r. and Jansson, Christer 2003. Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression. Journal of Plant Physiology, Vol. 160, Issue. 8, p. 921.

Satoh, Hikaru Nishi, Aiko Yamashita, Kazuhiro Takemoto, Yoko Tanaka, Yasumasa Hosaka, Yuko Sakurai, Aya Fujita, Naoko and Nakamura, Yasunori 2003. Starch-Branching Enzyme I-Deficient Mutation Specifically Affects the Structure and Properties of Starch in Rice Endosperm. Plant Physiology, Vol. 133, Issue. 3, p. 1111.

Sun, Chuanxin Palmqvist, Sara Olsson, Helena Borén, Mats Ahlandsberg, Staffan and Jansson, Christer 2003. A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of theiso1Promoter [W]. The Plant Cell, Vol. 15, Issue. 9, p. 2076.

Tanaka, Naoki Fujita, Naoko Nishi, Aiko Satoh, Hikaru Hosaka, Yuko Ugaki, Masashi Kawasaki, Shinji and Nakamura, Yasunori 2004. The structure of starch can be manipulated by changing the expression levels of starch branching enzyme IIb in rice endosperm. Plant Biotechnology Journal, Vol. 2, Issue. 6, p. 507.

Borén, Mats Larsson, Håkan Falk, Anders and Jansson, Christer 2004. The barley starch granule proteome—internalized granule polypeptides of the mature endosperm. Plant Science, Vol. 166, Issue. 3, p. 617.

Kosar‐Hashemi, Behjat Irwin, Jennifer A. Higgins, Jody Rahman, Sadequr and Morell, Matthew K. 2006. Isolation, identification and characterisation of starch‐interacting proteins by 2‐D affinity electrophoresis. ELECTROPHORESIS, Vol. 27, Issue. 9, p. 1832.

Han, Yuepeng Sun, Feng-Jie Rosales-Mendoza, Sergio and Korban, Schuyler S. 2007. Three orthologs in rice, Arabidopsis, and Populus encoding starch branching enzymes (SBEs) are different from other SBE gene families in plants. Gene, Vol. 401, Issue. 1-2, p. 123.

Han, Yuepeng Gasic, Ksenija Sun, Fengjie Xu, Mingliang and Korban, Schuyler S. 2007. A gene encoding starch branching enzyme I (SBEI) in apple (Malus×domestica, Rosaceae) and its phylogenetic relationship to Sbe genes from other angiosperms. Molecular Phylogenetics and Evolution, Vol. 43, Issue. 3, p. 852.

Han, Yuepeng Bendik, Elise Sun, Feng-Jie Gasic, Ksenija and Korban, Schuyler S. 2007. Genomic isolation of genes encoding starch branching enzyme II (SBEII) in apple: toward characterization of evolutionary disparity in SbeII genes between monocots and eudicots. Planta, Vol. 226, Issue. 5, p. 1265.

Download full list

Article contents

Identification of four starch-branching enzymes in barley endosperm: partial purification of forms I, IIa and IIb

Abstract

Keywords

Information

Access options

Article purchase

Temporarily unavailable

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Article contents

Identification of four starch-branching enzymes in barley endosperm: partial purification of forms I, IIa and IIb

Abstract

Keywords

Information

Access options

Article purchase

Temporarily unavailable

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests