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Identification of products formed by a fructan [ratio ] fructan fructosyltransferase activity from Lolium rigidum

Published online by Cambridge University Press:  01 February 1997

J. A. St. JOHN
Affiliation:
Department of Agriculture and Resource Management, The University of Melbourne, Parkville, Victoria, 3052, Australia
I. M. SIMS
Affiliation:
Department of Botany, Plant Cell Biology Research Centre, The University of Melbourne, Parkville, Victoria, 3052, Australia
G. D. BONNETT
Affiliation:
CSIRO Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia
R. J. SIMPSON
Affiliation:
CSIRO Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia
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Abstract

The products formed by a fructan [ratio ] fructan fructosyltransferase (FFT) activity purified from Lolium rigidumGaudin were identified after gas chromatography-mass spectrometry of partially methylated alditol acetates,electrospray ionization-mass spectrometry and reversed-phase high-performance liquid chromatography. TheFFT activity synthesized oligofructans up to degree of polymerization (DP) 6, but did not synthesize fructans ofDP > 6 even when assayed with (1,1,1)-kestopentaose for up to 10 h. The FFT activity when assayed with 1-kestose or 6G-kestose synthesized fructan with fructosyl residues almost exclusively linked by β-2,1-glycosidiclinkages. When assayed with 1-kestose, the FFT activity synthesized tetrasaccharides and pentasaccharides withan internal glucosyl residue. The predominant tetrasaccharide was (1&6G)-kestotetraose and the predominantpentasaccharide was (1&6G,1)-kestopentaose. By comparison, tetrasaccharides and pentasaccharides extractedfrom L. rigidum also contained predominantly β-2,1-glycosidic linked fructans with an internal glucosyl residue.The only exception was that one of the pentasaccharides contained β-2,1- and β-2,6-glycosidic linked fructosylresidues. This pentasaccharide was not synthesized by the FFT activity. The role of this FFT activity in formation of oligofructans in L. rigidum is discussed.

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Type
Research Article
Copyright
Trustees of the New Phytologist 1997

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