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The use of simple sequence repeat (SSR) markers to identify and map alien segments carrying genes for effective resistance to leaf rust in bread wheat

Published online by Cambridge University Press:  01 August 2007

Nayyer Iqbal ,
Firdissa Eticha ,
Elena K. Khlestkina ,
Annette Weidner ,
Marion S. Röder  and
Andreas Börner
Nayyer Iqbal
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Firdissa Eticha
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Elena K. Khlestkina
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Annette Weidner
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Marion S. Röder
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Andreas Börner*
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
*
*Corresponding author. E-mail: boerner@ipk-gatersleben.de
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Abstract

Aegilops markgrafii is a useful source of genes encoding both resistance to biotic stress and high seed lysine content. Bread wheat/Ae. markgrafii introgression lines expressing leaf rust resistance were developed from a cross between a leaf rust-resistant Ae.markgrafii accession and the susceptible bread wheat cultivar ‘Alcedo’. The content of introgressed segments present in five sister introgression lines was assessed with the help of chromosome-specific simple sequence repeats (SSRs). One of the lines was used as a parent of a 140 individual F2 mapping population, by crossing with the leaf rust-susceptible bread wheat cv. ‘Borenos’. The population was tested for susceptibility or resistance to leaf rust, and linkage analysis indicated the presence of a quantitative trait locus (QLr.ipk-2A) originating from the Ae. markgrafii parent, mapping to the distal segment of chromosome arm 2AS.

Keywords

Aegilops markgrafiidisease resistanceinterspecific introgression linesmicrosatellite markersPuccinia reconditaTriticum aestivum
Type
In Brief
Information
Plant Genetic Resources , Volume 5 , Issue 2 , August 2007 , pp. 100 - 103
Copyright
Copyright © NIAB 2007

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