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Assessment of genetic diversity and population structure of Indian common bean accessions using microsatellite markers

Published online by Cambridge University Press:  29 August 2023

Yogita Bisht ,
Himanshu Sharma Open the ORCID record for Himanshu Sharma [Opens in a new window] ,
Arun K. Jugran Open the ORCID record for Arun K. Jugran [Opens in a new window] ,
Ajay Veer Singh Open the ORCID record for Ajay Veer Singh [Opens in a new window] ,
Pankaj K. Mishra  and
Navneet Pareek
Yogita Bisht
Affiliation:
G. B. Pant National Institute of Himalayan Environment, Garhwal Regional Centre, Srinagar-246174, Uttarakhand, India
Himanshu Sharma
Affiliation:
Agri-Biotechnology Division, National Agri-Food Biotechnology Institute, Mohali, Punjab-140306, India
Arun K. Jugran*
Affiliation:
G. B. Pant National Institute of Himalayan Environment, Garhwal Regional Centre, Srinagar-246174, Uttarakhand, India
Ajay Veer Singh
Affiliation:
G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India
Pankaj K. Mishra
Affiliation:
ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, India
Navneet Pareek
Affiliation:
G. B. Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India
*
Corresponding author: Arun K. Jugran; Email: arunjugran@gbpihed.nic.in; arunjugran@gmail.com
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Abstract

Common bean (Phaseolus vulgaris L.) is an important crop of family Fabaceae used as a potential source of proteins, fibres and minerals. Thus, characterization of existing germplasm is useful for improvement and conservation. The Indian Himalayan Region harbours plentiful varieties of common bean, but it is nearly unexplored till date. In the present study, physical and genetic diversity of common bean was examined. Fifteen newly designed chloroplast microsatellite (cpSSR) markers were used to assess genetic diversity and population structure in 119 common bean individuals from 20 diverse accessions gathered from Uttarakhand, India. Significantly, positive (p< 0.05) relationship of seed weight was found with seed length (r = 0.813), seed width (r = 0.692) and seed length- width ratio (r = 0.694) using Pearson correlation analysis. A total of 20 alleles were identified using eight cpSSR markers. Mean number of alleles per locus (Na = 1.55), effective allele number (Ne = 1.370), expected heterozygosity (He = 0.213), average polymorphic loci (10.9) and Shannon information index (I = 0.313) were estimated based on cpSSR data. Maximum genetic diversity (He) was recorded in the AKJ/KK/DP/Jhalla/23 accession and minimum in the AKJ/YB/PS/Supi/43 accession. Bayesian-based STRUCTURE evaluation using cpSSR-based information partitioned 20 accessions into two distinct clusters which were also supported by neighbor-joining cluster analysis. These cpSSR markers also demonstrated transferability among other members like Vigna radiata, Macrotyloma uniflorum, Glycine max, Vigna mungo of Fabaceae family, therefore can be used to monitor their genetic heterogeneity. The findings from the study might be valuable to identify elite common bean accessions for production, conservation and future breeding programmes.

Keywords

accessionscommon beangenetic diversitypolymorphic information contenttransferability
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 2 , April 2023 , pp. 182 - 193
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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