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Assessment of genetic diversity in the USDA and CIP-FAO international nursery collections of quinoa (Chenopodium quinoa Willd.) using microsatellite markers

  • S. A. Christensen (a1), D. B. Pratt (a2), C. Pratt (a1), P. T. Nelson (a1), M. R. Stevens (a1), E. N. Jellen (a1), C. E. Coleman (a1), D. J. Fairbanks (a1), A. Bonifacio (a3) and P. J. Maughan (a1)...
Abstract

Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic diversity within quinoa germplasm and we use this set of 36 microsatellites markers to genetically characterize the diversity of 121 accessions of C. quinoa held in the USDA germplasm bank, 22 accessions from the CIP-FAO international nursery collection and eight accessions representing parents from genetic mapping populations. A total of 420 alleles were detected among the quinoa accessions with an average of 11 alleles detected per microsatellite locus. Genetic heterogeneity was observed in 32% of the quinoa accessions at a given locus and suggests that many of these accessions represent heterogeneous seed lots or landraces. Both unweighted pair-group method with arithmetic averages (UPGMA) and principle components analysis (PCA) analyses partitioned the quinoa accessions into two main clusters. The first major cluster consisted of accessions from the Andean highlands of Peru, Bolivia, Ecuador, Argentina and extreme northeastern Chile. The other main cluster contained accessions from both the lowlands of Chile and a set of USDA accessions with no known passport data, collected by Emigdio Ballón. Using the patterns of genetic diversity detected within the C. quinoa accessions we discuss hypotheses regarding quinoa's centre of diversity, including highland and lowland ecotype clustering patterns, origin of lowland varieties, origin of domestication, and diversity levels in the USDA and CIP-FAO collections.

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*Corresponding author. E-mail: jeff_maughan@byu.edu
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P Aellen (1929) Beitrag zur systematic der Chenopodium—Arten Amerikas, vorweigend auf Grund der Sammlung des United States National Museum in Washington, D.C. Feddes Repertorium Specierum Novarum Regni Vegetabilis 26: 3167, 119–160.

RE Dean , JA Dahlberg , MS Hopkins , SE Mitchell and S Kresovich (1999) Genetic redundancy and diversity among ‘orange’ accessions in the US National Sorghum Collection as assessed with simple sequence repeat (SSR) markers. Crop Science 39: 12151221.

N Diwan and PB Cregan (1997) Automated sizing of fluorescent-labeled simple sequence repeat (SSR) markers to assay genetic variation in soybean. Theoretical and Applied Genetics 95: 723733.

PK Gupta and RK Varshney (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with special emphasis on bread wheat. Euphytica 113: 163185.

SC Hokanson , AK Szewc-McFadden , WF Lamboy and JR McFerson (1998) Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus ×  domestica Borkh. core subset collection. Theoretical and Applied Genetics 97: 671683.

S Jain , RK Jain and SR McCouch Lima, Peru (2004) Genetic analysis of Indian aromatic and quality rice (Oryza sativa L.) germplasm using panels of fluorescently-labeled microsatellite markers. Theoretical and Applied Genetics 109: 965977.

ES Mace and ID Godwin (2002) Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta). Genome 45: 823832.

SL Mason , MR Stevens , EN Jellen , A Bonifacio , DJ Fairbanks , CE Coleman , RR McCarty , AG Rasmussen and PJ Maughan (2005) Development and use of microsatellite markers for germplasm characterization in quinoa (Chenopodium quinoa Willd.). Crop Science 45: 16181630.

PJ Maughan , MA Saghai Maroof , GR Buss and GM Huestis (1996) Amplified fragment length polymorphism (AFLP) in soybean: species diversity, inheritance, and near-isogenic line analysis. Theoretical and Applied Genetics 93: 392401.

CE McGregor , R van Treuren , R Hoekstra and TJL van Hintum (2002) Analysis of the wild potato germplasm of the series Acaulia with AFLPs: implications for exsitu conservation. Theoretical and Applied Genetics 104: 146156.

R Ortiz , EN Ruiz-Tapia and A Mujica-Sanchez (1998) Sampling strategy for a core collection of Peruvian quinoa germplasm. Theoretical and Applied Genetics 96: 475483.

DB Pratt and LG Clark (2001) Amaranthus rudis and A. tuberculatus—one species or two? Journal of the Torrey Botanical Society 128: 282296.

J Ruales and BM Nair (1992) Nutritional quality of the protein in quinoa (Chenopodium quinoa, Willd) seeds. Plant Foods and Human Nutrition 42: 111.

J Ruales , Y de Grijalva , P Lopez-Jaramillo and BM Nair (2002) The nutritional quality of an infant food from quinoa and its effect on the plasma level of insulin-like growth factor-1 (IGF-1) in undernourished children. International Journal of Food Science and Nutrition 53: 143154.

NW Simmonds (1965) The grain chenopods of the tropical American highlands. Economic Botany 19: 223235.

NW Simmonds (1971) The breeding system of Chenopodiumquinoa. I. Male sterility. Heredity 27: 7382.

JJ Todd and LO Vodkin (1996) Duplications that suppress and deletions that restore expression from a chalcone synthase multigene family. Plant Cell 8: 687699.

SM Ward (2000) Allotetraploid segregation for single-gene morphological characters in quinoa (Chenopodium quinoa Willd.). Euphytica 116: 1116.

HD Wilson (1988a) Quinoa biosystematics I: domesticated populations. Economic Botany 42: 461477.

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Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
  • URL: /core/journals/plant-genetic-resources
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