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Comparative assessment of SCoT and ISSR markers for analysis of genetic diversity and population structure in some Aegilops tauschii Coss. accessions

Published online by Cambridge University Press:  13 October 2021

Atefeh Nouri ,
Maryam Golabadi Open the ORCID record for Maryam Golabadi [Opens in a new window] ,
Alireza Etminan ,
Abdolmajid Rezaei  and
Ali Ashraf Mehrabi Open the ORCID record for Ali Ashraf Mehrabi [Opens in a new window]
Atefeh Nouri
Affiliation:
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Maryam Golabadi*
Affiliation:
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Alireza Etminan
Affiliation:
Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
Abdolmajid Rezaei
Affiliation:
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Ali Ashraf Mehrabi
Affiliation:
Department of Biotechnology, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
*
Author for correspondence: Maryam Golabadi, E-mail: golabadim@gmail.com
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Abstract

Aegilops tauschii, the diploid progenitor of the wheat D-genome, is a valuable genetic resource for wheat breeders. In this study, we compared the efficiency of inter-simple sequence repeat (ISSR) (as an arbitrary technique) and start codon targeted (SCoT) (as a gene-targeting technique) markers in determining the genetic diversity and population structure of 90 accessions of Ae. tauschii. SCoT markers indicated the highest values for polymorphism information content, marker index and effective multiplex ratio compared to ISSR markers. The total genetic diversity (Ht) and genetic diversity within populations (Hs) parameters were comparably modest for the two marker systems. The results of the analysis of molecular variance showed that the genetic variation within populations was significantly higher than among them (ISSR: 92 versus 8%; SCoT: 88 versus 12%). Furthermore, SCoT markers discovered a high level of genetic differentiation among populations than ISSRs (0.19 versus 0.05), while the amount of gene flow detected by ISSR was higher than SCoT (2.13 versus 8.62). Cluster analysis and population structure of SCoT and ISSR data divided all investigated accessions into two and four main clusters, respectively. Our results revealed that SCoT and ISSR fingerprinting could be used to further molecular analysis in Ae. tauschii and other wild species. The high-genetic variability found in this study also indicates the valuable genetic potential present in the investigated Ae. tauschii germplasm, which could be utilized for future genetic analysis and linkage mapping in breeding programmes.

Keywords

Aegilopscomparative marker assessmentcrop wild relativeswheat
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 5 , October 2021 , pp. 375 - 383
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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