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    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Crespan, M. Carraro, R. Giust, M. and Migliaro, D. 2016. Origin of Termarina cultivar, another grapevine (Vitis viniferaL.) parthenocarpic somatic variant. Australian Journal of Grape and Wine Research,

    Muzzalupo, Innocenzo Pisani, Francesca Greco, Federica and Chiappetta, Adriana 2015. Direct DNA amplification from virgin olive oil for traceability and authenticity. European Food Research and Technology, Vol. 241, Issue. 1, p. 151.

    Zarouri, Belkacem Vargas, Alba María Gaforio, Laura Aller, María de Andrés, María Teresa and Cabezas, José Antonio 2015. Whole-genome genotyping of grape using a panel of microsatellite multiplex PCRs. Tree Genetics & Genomes, Vol. 11, Issue. 2,

    Migliaro, D. Crespan, M. Muñoz-Organero, G. Velasco, R. Moser, C. and Vezzulli, S. 2014. Structural dynamics at the berry colour locus inVitis vinifera L. somatic variants. Australian Journal of Grape and Wine Research, Vol. 20, Issue. 3, p. 485.


Direct multiplex PCR for grapevine genotyping and varietal identification

  • Daniele Migliaro (a1), Giacomo Morreale (a1), Massimo Gardiman (a1), Sara Landolfo (a1) and Manna Crespan (a1)
  • DOI:
  • Published online: 05 December 2012

Grapevine cultivar identification is based mainly on two complementary methodologies: microsatellite (simple sequence repeat (SSR)) DNA analysis and traditional ampelography. Here, we report a direct multiplex PCR approach that allows the simultaneous amplification of 11 SSR loci from crude samples, i.e. bypassing DNA extraction, by using an engineered DNA polymerase improved to tolerate plant PCR inhibitors. Many different plant tissues were successfully amplified: leaf, root, wood, berry flesh and skin, stalk and must, from wine and table grape varieties, and rootstocks. The direct multiplex PCR that we propose is quicker and cheaper than the methodologies used until now, and provides accurate results. Thus, SSR DNA analysis becomes economically more accessible to a larger number of potential users in addition to research institutes.

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Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
  • URL: /core/journals/plant-genetic-resources
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