Skip to main content
×
×
Home

A DNA fingerprinting-based taxonomic allocation of Kamut wheat

  • Elena K. Khlestkina (a1) (a2), Marion S. Röder (a1), Heinrich Grausgruber (a3) and Andreas Börner (a1)
Abstract

Kamut wheat, said to have been derived from seed found in the Egyptian pyramids, appeared on the market about 25 years ago. We have investigated its taxonomic placement using microsatellite genotyping. In all, 89 accessions of 13 tetraploid wheat species, along with samples of Kamut wheat, were genotyped using two A and B genome wheat microsatellite markers per chromosome, generating 453 alleles (8–33 alleles per locus), and a mean allelic polymorphic information content (PIC) of 0.80. A diversity analysis showed that nine major accession groups could be defined, and these were inconsistent with formal taxonomic classifications of about 10% of the material. Most of these misclassifications are due to either species introgression or seed admixture. Some accessions appear to be duplicates. The Kamut wheats grouped together in a cluster containing three accessions of Triticum polonicum and three of T. durum, originating from Turkey, Iraq, Iran and Israel. We suggest that Kamut perhaps derived from a natural hybrid between T. durum and T. polonicum, which occurred in the Fertile Crescent.

Copyright
Corresponding author
* E-mail: boerner@ipk-gatersleben.de
References
Hide All
Alamerew, S, Chebotar, S, Huang, XQ, Röder, MS and Börner, A (2004) Genetic diversity in Ethiopian hexaploid and tetraploid wheat germplasm assessed by microsatellite markers. Genetic Resources and Crop Evolution 51: 559567.
Anderson, JA, Churchill, GA, Antrique, JE, Tanksley, SD and Sorrels, ME (1993) Optimising parental selection for genetic linkage maps. Genome 36: 181188.
Ben, Amer IM, Börner, A and Röder, MS (2001) Detection of genetic diversity in Libyan wheat genotypes using wheat microsatellite markers. Genetic Resources and Crop Evolution 48: 579585.
Chebotar, SV and Sivolap, YM (2001) Differentiation, identification and characterization of Triticum aestivum L. varieties from Ukrainian breeding programs by using STMS analysis. Cytology and Genetics 35: 1827.(in Russian)
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297302.
Donini, P, Stephenson, P, Bryan, GJ and Koebner, RMD (1998) The potential of microsatellites for high throughput genetic diversity assessment in wheat and barley. Genetic Resources and Crop Evolution 45: 415421.
Dorofeev, VF, Filatenko, AA, Migushova, EF, Udachin, RA andJakubziner, MM (1979) Flora of Cultivated Plants. Leningrad: Kolos, Leningrad Branch (in Russian).
Fahima, T, Röder, MSGrama, A and Nevo, E (1998) Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theoretical and Applied Genetics 96: 187195.
Hammer, K, Filatenko, AA and Korzun, V (2000) Microsatellite markers–a new tool for distinguishing diploid wheat. Genetic Resources and Crop Evolution 47: 497505.
Huang, XQ, Börner, A, Röder, MS and Ganal, MW (2002) Assessing genetic diversity of wheat ( Triticum aestivum L.) germplasm using microsatellite markers. Theoretical and Applied Genetics 105: 699707.
Kasarda, DD (2001) Grains in relation to celiac disease. Cereal Foods World 46: 209210.
Khlestkina, EK, Huang, XQ, Quenum, FJBChebotar, S, Röder, MS and Börner, A (2004 a) Genetic diversity in cultivated plants–loss or stability?. Theoretical and Applied Genetics 108: 14661472.
Khlestkina, EK, Röder, MS, Efremova, TT, Börner, A and Shumny, VK (2004b) The genetic diversity of old and modern Siberian varieties of common spring wheat determined by microsatellite markers. Plant Breeding 123: 122127.
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA 70: 33213323.
Plaschke, J, Ganal, MW and Röder, MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics 91: 10011007.
Quinn, RM (1999) Kamut ® ancient grain, new cereal. in Janick, J (ed.) Perspectives on New Crops and New Uses. Alexandria, VA: ASHS Press, pp 182183.
Röder, MS, Plaschke, J, König, SU, Börner, A, Sorrells, ME, Tanksley, SD and Ganal, MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246: 327333.
Röder, MS, Korzun, V, Wendehake, K, Plaschke, J, Tixier, M-H, Leroy, P and Ganal, MW (1998) A microsatellite map of wheat. Genetics 149: 20072023.
Röder, MS, Wendehake, K, Korzun, V, Bredemeijer, G, Laborie, D, Bertrand, L, Isaak, P, Rendell, S, Jackson, J, Cooke, RJ, Vosman, B and Ganal, MW (2002) Construction and analysis of a microsatellite-based database of European wheat varieties. Theoretical and Applied Genetics 106: 6773.
Rohlf, FJ (1998) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, version 2.0. New York: Applied Biostatistics.
Simonato, B, Pasini, G, Giannattasio, M and Curioni, A (2002) Allergenic potential of Kamut wheat. Allergy 57: 653654.
Stallknecht, GF, Gilbertson, KM and Ranney, JE (1996) Alternative wheat cereals as food grains: Einkorn, Emmer, Spelt, Kamut, and Triticale. In: Janick, J (ed.) Progress in New Crops Alexandria, VA: ASHS Press. 156170.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
  • URL: /core/journals/plant-genetic-resources
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed