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Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

Published online by Cambridge University Press:  07 January 2022

Susheel Sharma Open the ORCID record for Susheel Sharma [Opens in a new window] ,
Aejaz Ahmad Dar ,
Sachin Gupta  and
Ravinder Singh
Susheel Sharma*
Affiliation:
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, Jammu, India
Aejaz Ahmad Dar
Affiliation:
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, Jammu, India
Sachin Gupta
Affiliation:
Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, Jammu, India
Ravinder Singh
Affiliation:
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Chatha 180009, Jammu, India
*
Author for correspondence: Susheel Sharma, E-mail: drsusheelsharma@rediffmail.com
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Abstract

Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.

Keywords

breedingcucumberdisease resistancemolecular markerspowdery mildew
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 6 , December 2021 , pp. 497 - 502
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

These authors contributed equally to the manuscript

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