Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-28T14:16:13.069Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic relatedness and cultivar identification in a valuable garden species, Hesperantha coccinea (Schizostylis coccinea)

Published online by Cambridge University Press:  07 May 2009

Kirsten Wolff ,
Sabina Knees  and
Suzanne Cubey
Kirsten Wolff*
Affiliation:
School of Biology, Newcastle University, Ridley Building, Newcastle upon TyneNE1 7RU, UK
Sabina Knees
Affiliation:
Royal Botanic Garden Edinburgh, 20A Inverleith Row, EdinburghEH3 5LR, UK
Suzanne Cubey
Affiliation:
Royal Botanic Garden Edinburgh, 20A Inverleith Row, EdinburghEH3 5LR, UK
*
*Corresponding author. E-mail: kirsten.wolff@ncl.ac.uk
Get access Rights & Permissions [Opens in a new window]

Abstract

DNA fingerprinting using microsatellites is a useful aid in cultivar identification, but has rarely been applied to garden plants. Eleven microsatellite markers were developed for the valuable garden plant Hesperanthacoccinea (Schizostylis coccinea), and used to determine relatedness of accessions. Several accessions, described as separate cultivars, appeared to have identical genotypes. Among the 53 accessions tested, there were 34 unique multilocus genotypes. The level of polymorphism detected in the cultivars was high, with on average seven alleles per locus and an average expected heterozygosity of 0.72 across loci. It is clear from the genotypes that a large proportion of the cultivars are closely related to each other. The resulting markers can now be used to generate a complete database of all known cultivars of the species and to detect essentially derived cultivars. As an extension of this study, the markers identified here could also inform us about the genetic diversity in wild populations.

Keywords

cultivar identificationHesperantha, Schizostylismicrosatellitesrelatedness
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 3 , December 2009 , pp. 281 - 290
Copyright
Copyright © NIAB 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, R, Okada, M, Firestone, JL, Mallek, CR and Jasieniuk, M (2006) Isolation, characterization, and evaluation of microsatellite loci for cultivar identification in the ornamental pampas grass Cortaderia selloana. Journal of the American Society of Horticultural Sciences 131: 499–505.CrossRefGoogle Scholar
Becher, SA, Steinmetz, K, Weising, K, Boury, S, Peltier, D, Renou, J-P, Kahl, G and Wolff, K (2000) Microsatellites for cultivar identification in Pelargonium. Theoretical and Applied Genetics 101: 643–651.CrossRefGoogle Scholar
Brickell, CD, Baum, BR, Hetterscheid, WLA, Leslie, AC, McNeill, J, Trehane, P, Vrugtman, F and Wiersema, JH (2004) International code of nomenclature for cultivated plants: introductory pages. ISHS Acta Horticulturae 647: 1–123.Google Scholar
Edwards, KJ, Barker, JHA, Daly, A, Jones, C and Karp, A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20: 758–760.CrossRefGoogle ScholarPubMed
Esselink, GD, Smulders, MJM and Vosman, B (2003) Identification of cut rose (Rosa hybrida) and rootstock varieties using robust sequence tagged microsatellite site markers. Theoretical and Applied Genetics 106: 277–286.CrossRefGoogle ScholarPubMed
Excoffier, L, Laval, G and Schneider, S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolution and Bioinformatics Online 1: 47–50.Google Scholar
Goldblatt, P (2003) A synoptic review of the African genus Hesperantha (Iridaceae: Crocoideae). Annals of the Missouri Botanical Garden 90: 431.CrossRefGoogle Scholar
Goldblatt, P and Manning, JC (1996) Reduction of Schizostylis (Iridaceae: Ixioideae) in Hesperantha. Novon 6: 262–264.CrossRefGoogle Scholar
Goldblatt, P and Manning, JC (2008) The Iris Family. Portland, OR: Timber Press.Google Scholar
Hale, ML, Bevan, R and Wolff, K (2001) New polymorphic microsatellite markers for the red squirrel (Sciurus vulgaris) and their application to the grey squirrel (S. carolinensis). Molecular Ecology Notes 1: 47–49.CrossRefGoogle Scholar
Hale, ML, Borland, AM and Wolff, K (2005) Lack of phylogenetic signal of highly transferable microsatellites in Clusia. Genome 48: 946–950.CrossRefGoogle Scholar
Huxley, A (ed.) (1992) New RHS dictionary of Gardening. London: Macmillan.Google Scholar
Kalinowski, ST, Wagner, AP and Taper, ML (2006) Ml-Relate: a computer program for maximum likelihood estimation of relatedness and relationship. Molecular Ecology Notes 6: 576–579.CrossRefGoogle Scholar
Kimura, T, Nishitani, C, Iketani, H, Ban, Y and Yamamoto, T (2006) Development of microsatellite markers in rose. Molecular Ecology Notes 6: 810–812.CrossRefGoogle Scholar
Lord, T, Armitage, J, Cubey, J, Edwards, D, Lancaster, N and Merrick, J (2008) RHS Plant Finder 2008–2009. London: Dorling Kindersley.Google Scholar
Lubell, JD, Brand, MH and Lehrer, JM (2008) Identification of Berberis thunbergii cultivars, inter-specific hybrids, and their parental species. Journal of Horticultural Science and Biotechnology 83: 55–63.CrossRefGoogle Scholar
Parks, EJ, Moyer, JW and Lyerly, JH (2006) Identification of fluorescent AFLP and SSR markers for differentiation and analysis of New Guinea Impatiens. Journal of the American Society for Horticultural Science 131: 622–631.CrossRefGoogle Scholar
Peakall, R and Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.CrossRefGoogle Scholar
Pharmawati, M, Yan, G and Finnegan, PM (2005) Molecular variation and fingerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers. Annals of Botany 95: 1163–1170.CrossRefGoogle ScholarPubMed
Reeves, G, Chase, MW, Goldblatt, P, Rudall, P, Fay, MF, Cox, AV, Lejeune, B and Souza-Chies, T (2001) Molecular systematics of Iridaceae: evidence from four plastid DNA regions. American Journal of Botany 88: 2074–2087.CrossRefGoogle ScholarPubMed
Rousset, F and Raymond, M (1995) Testing heterozygote excess and deficiency. Genetics 140: 1413–1419.CrossRefGoogle ScholarPubMed
Rozen, S and Skaletsky, HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz, S and Misener, S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press, pp. 365–386.Google Scholar
Scariot, V, Akkak, A and Botta, R (2006) Characterization and genetic relationships of wild species and old garden roses based on microsatellite analysis. Journal of the American Society for Horticultural Science 131: 66–73.CrossRefGoogle Scholar
Smouse, PE and Peakall, R (1999) Spatial autocorrelation analysis of individual multiallele and multilocus genetic structure. Heredity 82: 561–573.CrossRefGoogle ScholarPubMed
Straley, GB (1989) Schizostylis - cultivation and biology. Herbertia 45.Google Scholar
Weising, K, Nybom, H, Wolff, K and Kahl, G (2005) DNA Fingerprinting in Plants: Principles, Methods and Applications. Boca Raton, FL: CRC Press, p. 496.Google Scholar
Wolff, K (1996) RAPD analysis of sporting and chimerism in chrysanthemum. Euphytica 89: 159–164.CrossRefGoogle Scholar
Wright, S (1969) Evolution and the Genetics of Populations Vol. II: The Theory of Gene Frequencies. Chicago, IL: University of Chicago Press.Google Scholar