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Genomic resources for Dyckia ibiramensis: A set of microsatellite markers and the plastid genome sequence of a threatened Brazilian bromeliad

Published online by Cambridge University Press:  29 July 2025

Joana Nascimento Oliveira Zeist Open the ORCID record for Joana Nascimento Oliveira Zeist [Opens in a new window] ,
Liana Bulcão Bittencourt Petrarca ,
Yohan Fritsche ,
Ana K. de Sousa Silva ,
André Ricardo Zeist ,
Tiago Montagna  and
Valdir Marcos Stefenon Open the ORCID record for Valdir Marcos Stefenon [Opens in a new window]
Joana Nascimento Oliveira Zeist*
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil
Liana Bulcão Bittencourt Petrarca
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil
Yohan Fritsche
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil
Ana K. de Sousa Silva
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil
André Ricardo Zeist
Affiliation:
Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil
Tiago Montagna
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil
Valdir Marcos Stefenon
Affiliation:
Department of Plant Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil
*
Corresponding author: Joana Nascimento Oliveira Zeist; Email: joanazeist@gmail.com
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Abstract

Dyckia ibiramensis is an endemic species from southern Brazil, known for its ability to adapt to extreme environmental variations. This study reports the development of species-specific microsatellite markers and the assembly and annotation of the plastid genome of D. ibiramensis, aiming to generate new genomic resources useful for studies on the conservation and evolution of this endangered species. A total of 33,112 microsatellite loci were identified, of which 10 were selected and validated for genotyping 30 individuals from natural populations, showing high genetic variability. These 10 microsatellite markers were very informative for the evaluation of genetic variability. These microsatellite markers evidenced moderate to high genetic diversity at the individual level, low population differentiation, and the capacity of the species to recover population size from ancient genetic bottlenecks. The assembled plastid genome revealed conserved structures and the occurrence of features at the gene level, likely related to stress response to environmental conditions. This study expands the understanding of the genetics of D. ibiramensis, highlighting the importance of genomic strategies for the conservation of endangered species. In situ and ex situ conservation strategies should be used to avoid the extinction of this endemic southern Brazilian genetic resource in nature.

Keywords

genetic conservationgenetic diversitygenomic variabilityphylogenomicsSSRs

Information

Type
Research Article
Information
Plant Genetic Resources , First View , pp. 1 - 12
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany.

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