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Identification of root morphology mutants in barley

Published online by Cambridge University Press:  16 March 2011

Riccardo Bovina ,
Valentina Talamè ,
Matteo Ferri ,
Roberto Tuberosa ,
Beata Chmielewska ,
Iwona Szarejko  and
Maria Corinna Sanguineti
Riccardo Bovina
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Valentina Talamè
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Matteo Ferri
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Roberto Tuberosa
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Beata Chmielewska
Affiliation:
Department of Genetics, University of Silesia, Poland
Iwona Szarejko
Affiliation:
Department of Genetics, University of Silesia, Poland
Maria Corinna Sanguineti*
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
*
*Corresponding author. E-mail: maria.sanguineti@unibo.it
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Abstract

In this study, a forward-genetics analysis was performed on a portion of TILLMore, a chemically mutagenized population of barley cv. ‘Morex’ (http://www.distagenomics.unibo.it/TILLMore/), to identify root morphology alterations by comparison with ‘Morex’ wild-type. For this purpose, a simple paper-roll approach was performed to identify phenotypic variants at the seedling stage. The analysis of c. 1000 M4 families allowed us to identify c. 70 lines with altered root morphology. A more accurate phenotypic characterization of a portion of the mutant lines has been performed using stereomicroscopy and a scanning electron microscopy approach.

Keywords

forward-geneticsHordeum vulgareroot hairsroot morphologyTILLING

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 357 - 360
Copyright
Copyright © NIAB 2011

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