Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-05-11T09:31:17.768Z Has data issue: false hasContentIssue false
  • English
  • Français

Rice blast resistance gene profiling of Thai, Japanese and international rice varieties using gene-specific markers

Published online by Cambridge University Press:  12 May 2022

Wattanaporn Teerasan ,
Pattaraborn Moonsap ,
Apinya Longya ,
Katanyutita Damchuay ,
Shin-ichi Ito ,
Piyama Tasanasuwan ,
Sureeporn Kate-Ngam  and
Chatchawan Jantasuriyarat Open the ORCID record for Chatchawan Jantasuriyarat [Opens in a new window]
Wattanaporn Teerasan
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Kasetsart University, Bangkok 10900, Thailand
Pattaraborn Moonsap
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Apinya Longya
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Katanyutita Damchuay
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Shin-ichi Ito
Affiliation:
Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan
Piyama Tasanasuwan
Affiliation:
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Sureeporn Kate-Ngam*
Affiliation:
Department of Agronomy, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand
Chatchawan Jantasuriyarat*
Affiliation:
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Kasetsart University, Bangkok 10900, Thailand Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart (CASTNAR, NRU-KU), Kasetsart University, Bangkok 10900, Thailand
*
Author for correspondence: Chatchawan Jantasuriyarat, E-mail: fscicwj@ku.ac.th; Sureeporn Kate-Ngam, E-mail: sureeporn.k@ubu.ac.th
Author for correspondence: Chatchawan Jantasuriyarat, E-mail: fscicwj@ku.ac.th; Sureeporn Kate-Ngam, E-mail: sureeporn.k@ubu.ac.th
Get access Rights & Permissions [Opens in a new window]

Abstract

Rice blast disease, caused by Magnaporthe oryzae, is one of the most damaging diseases of rice worldwide. Cultivation of rice varieties carrying resistance genes is the most economic and successful strategy to control the disease. In this study, 451 rice varieties from around the world including 363 Thai landrace rice varieties, 21 Thai improved rice varieties, 43 Japanese rice varieties and 24 worldwide rice varieties were screened by PCR technique using gene-specific markers for 10 rice blast resistance genes: Pi9, Piz-t, Pi50, Pigm(t), Pid2, Pid3, Pia, Pik, Pi54 and Pita. The results showed that 382 (99.48%) Thai rice varieties have at least one resistance gene and two rice varieties, ‘Hom’ and ‘Bak muay’, contained eight out of ten screened rice blast resistance genes. 320 rice varieties (83.33%) contained three or more rice blast resistance genes. The frequency of the rice blast resistance gene ranges from 87.76–9.64 per cent, of which the Pid3 gene has the highest frequency and the Pi54 gene has the lowest frequency. Two major resistance genes, found in Japanese rice varieties, are the Pik gene (76.74%) and the Pi9 gene (72.09%). While two major resistance genes, found in the international rice varieties are the Pi9 gene (66.67%) and the Pi54 gene (62.50%). The disease resistance gene profile of each rice variety obtained from this study will benefit the rice blast resistant breeding programme in the future.

Keywords

Blast diseaseblast fungusgenetic diversityMagnaporthe oryzaeOryza sativa
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 22 - 28
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ali, A, Hyun, DY, Choi, YM, Lee, S, Oh, S, Park, HJ and Lee, MC (2016) Screening of rice germplasm for the distribution of rice blast resistance genes and identification of resistant sources. Korean Journal of Plant Research 29, 658–669.CrossRefGoogle Scholar
Ariya-anandech, K, Chaipanya, C, Teerasan, W, Kate-Ngam, S and Jantasuriyarat, C (2018) Detection and allele identification of rice blast resistance gene, Pik, in Thai rice germplasm. Agriculture and Natural Resources 52, 525–535.CrossRefGoogle Scholar
Bryan, GT, Wu, K, Farrall, L, Jia, Y, Hershey, HP, McAdams, SA, Faulk, KN, Donaldson, GK, Tarchini, K and Valent, B (2000) A single amino acid difference distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-ta. Plant Cell 12, 2033–2045.Google ScholarPubMed
Chaipanya, C, Telebanco-Yanoria, MJ, Quime, B, Longya, A, Korinsak, S, Korinsak, S, Toojinda, T, Vanavichit, A, Jantasuriyarat, C and Zhou, B (2017) Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast. Rice 10, 18.CrossRefGoogle ScholarPubMed
Chen, XW, Li, SG, Xu, JC, Zha, WX, Ling, ZZ, Ma, BT, Wang, YP, Wang, WM, Cao, G, Ma, YQ, Shang, JJ, Zhao, XF, Zhou, KD and Zhu, LH (2004) Identification of two blast resistance genes in a rice variety, Digu. Phytopathology 152, 77–85.CrossRefGoogle Scholar
Deng, Y, Zhu, X, Shen, Y and He, Z (2006) Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad spectrum resistant Chinese variety. Theoretical and Applied Genetics 113, 705–713.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19, 11–15.Google Scholar
Flor, HH (1971) Current status of gene-for-gene concept. Annual Review of Phytopathology 9, 275–296.CrossRefGoogle Scholar
Gururani, MA, Venkatesh, J, Upadhyaya, CP, Nookaraju, A, Pandey, SK and Park, SW (2012) Plant disease resistance genes: current status and future directions. Physiological and Molecular Plant Pathology 78, 51–65.CrossRefGoogle Scholar
Hayashi, K, Yoshida, H and Ashikawa, I (2006) Development of PCR-based allele specific and InDel marker sets for nine rice blast resistance gene. Theoretical and Applied Genetics 113, 251–260.CrossRefGoogle Scholar
Imam, J, Alam, S, Mandal, NP, Variar, M and Shukla, P (2014) Molecular screening for identification of blast resistance genes in north east and eastern Indian rice germplasm (Oryza sativa L.) with PCR based markers. Euphytica 196, 199–211.CrossRefGoogle Scholar
Jia, Y, Wang, Z and Singh, P (2002) Development of dominant rice blast Pi-ta resistance gene markers. Crop Science 42, 2145–2149.CrossRefGoogle Scholar
Kalia, S and Rathour, R (2019) Current status on mapping of genes for resistance to leaf- and neck-blast disease in rice. Biotech 9, 209.Google ScholarPubMed
Kim, JS, Ahn, SN, Kim, CK and Shim, CK (2010) Screening of rice blast resistance genes from aromatic rice germplasms with SNP markers. Plant Pathology Journal 26, 70–79.CrossRefGoogle Scholar
Koide, Y, Kobayashi, N, Xu, D and Fukuta, Y (2009) Resistance gene and selection DNA marker for blast disease in rice (Oryza sativa L.). Japan Agricultural Research Quarterly 43, 255–280.CrossRefGoogle Scholar
Liang, Y, Yan, BY, Peng, YL, Ji, ZJ, Zeng, YX, Wu, HL and Yang, CD (2017) Molecular screening of blast resistance genes in rice germplasms resistant to Magnaporthe oryzae. Rice Science 24, 41–47.Google Scholar
Liu, G, Lu, G, Zen, L and Wang, GL (2002) Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t) are physically linked on rice chromosome 6. Molecular Genetics and Genomics 267, 472–480.CrossRefGoogle ScholarPubMed
Longya, A, Chaipanya, C, Franceschetti, M, Maidment, JHR, Banfield, MJ and Jantasuriyarat, C (2019) Gene duplication and mutation in the emergence of a novel aggressive allele of the AVR-Pik effector in the rice blast fungus. Molecular Plant-Microbe Interactions 32, 740–749.CrossRefGoogle ScholarPubMed
Moonsap, P, Laksanavilat, N, Tasanasuwan, P, Kate-Ngam, S and Jantasuriyarat, C (2019) Assessment of genetic variation of 15 Thai elite rice cultivars using InDel markers. Crop Breeding and Applied Biotechnology 19, 15–21.CrossRefGoogle Scholar
Nalampangnoenplab, A (2011) Minimization of rice blast severity by means of multilines in the lower north. In Proceedings of Rice Research Symposium 2011. Rice Research Center Groups in Upper and Lower Northern Region, Phrae, Thailand, pp. 225–241.Google Scholar
Phaitreejit, K, Srikaew, E, Jantasuriyarat, C, Sreewongchai, T and Katengam, S (2011) Screening Thai landrace rice for blast resistance gene Pi9, Pi36, Pigm(t) using DNA markers. Thai Journal of Genetics 4, 52–62.Google Scholar
Promchuay, A and Nilthong, S (2017) Investigation of Pid3 rice blast resistant gene in Northern upland rice varieties (Oryza sativa L.), Thailand using molecular markers. Journal of Advanced Agricultural Technologies 4, 209–214.CrossRefGoogle Scholar
Ramkumar, G, Srinivasarao, K, Madhan, MK, Sudarshan, I, Sivaranjani, AKP, Gopalakrishna, K, Neeraja, CN, Balachandran, SM, Sundaram, RM, Prasad, MS, Shobh, N, Rama, AM, Viraktamath, BC and Madhav, MS (2011) Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pikh). Molecular Breeding 27, 129–135.CrossRefGoogle Scholar
Shang, J, Tao, Y, Chen, X, Zou, Y, Lei, C, Wang, J, Li, X, Zhao, X, Zhang, M, Lu, Z, Xu, J, Cheng, Z, Wan, J and Zhu, L (2009) Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes. Genetics 182, 1303–1311.CrossRefGoogle Scholar
Sharma, TR, Rai, AK, Gupta, SK, Vijayan, J, Devanna, BN and Ray, S (2012) Rice blast management through host-plant resistance: retrospect and prospects. Agricultural Research 1, 37–52.CrossRefGoogle Scholar
Singh, AK, Singh, PK, Arya, M, Singh, NK and Singh, US (2015) Molecular screening of blast resistance genes in rice using SSR markers. Plant Pathology Journal 31, 12–24.CrossRefGoogle ScholarPubMed
Srikeaw, I (2010) Investigation and Searching of Rice Blast Resistance Gene in Landrace Thai Rice Using DNA Marker (M.S). Kasetsart University, Thailand.Google Scholar
Teerasan, W, Srikaew, I, Phaitreejit, K, Kate-Ngam, S and Jantasuriyarat, C (2019) Gene-specific marker screening and disease reaction validation of blast resistant genes, Pid3, Pigm and Pi54 in Thai landrace rice germplasm and recommended rice varieties. Plant Genetic Resources 17, 421–426.CrossRefGoogle Scholar
Tharachand, C, Immanuel, SC and Mythili, MN. (2012) Molecular markers in characterization of medicinal plants: an overview. Research in Plant Biology 2, 1–12.Google Scholar
The Global Rice Science Partnership (GRiSP) (2013) Rice Almanac, 4th Edn, Thailand: The Global Rice Science Partnership, CGIAR, pp. 134–138.Google Scholar
Wang, X, Lee, S, Wang, J, Ma, J, Bianco, T and Ji, Y (2014) Current advances on genetic resistance to rice blast disease. In Yan, W and Bao, J (eds), Rice – Germplasm, Genetics and Improvement. Rijeka: IntechOpen. pp. 195–217. Available at https://doi.org/10.5772/56824.Google Scholar
Wongsaprom, C, Sirithunya, P, Vanavichit, A, Pantuwan, G, Jongdee, B, Sidhiwong, N, Lanceras-Siangliw, J and Toojinda, T (2010) Two introgressed quantitative trait loci confer a broad-spectrum resistance to blast disease in the genetic background of the cultivar RD6 a Thai glutinous jasmine rice. Field Crops Research 119, 245–251.CrossRefGoogle Scholar
Xiao, G, Borja, FN, Mauleon, R, Padilla, J, Telebanco-Yanoria, MJ, Yang, J, Lu, G, Dionisio-Sese, M and Zhou, B (2017) Identification of resistant germplasm containing novel resistance genes at or tightly linked to the Pi2/9 locus conferring broad-spectrum resistance against rice blast. Rice 10, 37.CrossRefGoogle ScholarPubMed
Yadav, S and Kumar, V (2018) Feeding the world while caring for the planet. DSRC Newsletter 1, 3–4.Google Scholar
Supplementary material: File

Teerasan et al. supplementary material

Tables S1-S2

Download Teerasan et al. supplementary material(File)
File 126.1 KB