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Validation of QTLs associated with spikelets per panicle and grain weight in rice

  • Sang-Min Yeo (a1), Yeo-Tae Yun (a2), Dong-Min Kim (a1), Chong-Tae Chung (a2) and Sang-Nag Ahn (a1)...


In this study, a near-isogenic line (BC4F10) CR572 developed by introgressing a chromosomal segment from Oryza rufipogon (accession no. 105491) into the Oryza sativa subsp. japonica cv. Hwaseong was found to exhibit a significant increase in the number of spikelets per panicle (SPP) and grain weight compared with the recurrent parent Hwaseong. Quantitative trait locus (QTL) analysis in F2 generation derived from the cross between CR572 and Hwaseong revealed that two QTLs, qSPP1 and qTGW1, were linked to a simple sequence repeat marker, RM283, on chromosome 1. The additive effect of the O. rufipogon allele at qSPP1 was 13 SPP, and 21.6% of the phenotypic variance was explained by the segregation of RM283. The qTGW1 QTL explained 19.1% of the phenotypic variance for grain weight. Substitution mapping was carried out with five F3 lines derived from F2 plants having informative recombination breakpoints within the target region. Substitution mapping indicated the linkage of qSPP1 and qTGW1. The grain yield of CR572 was 18.2 and 15.8% higher than that of Hwaseong at two locations, respectively, mainly due to the increase in 1000-grain weight and SPP. These results are very useful for QTL cluster transfer by molecular marker-assisted selection in rice breeding programmes and for QTL gene cloning by map-based cloning.


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Ashikari, M, Sakakibara, H, Lin, SY, Yamamoto, T, Takashi, T, Nishimura, A, Angeles, ER, Qian, Q, Kitano, H and Matsuoka, M (2005) Cytokinin oxidase regulates rice grain production. Science 309: 741745.
Causse, MA, Fulton, TM, Cho, YG, Ahn, SN, Chunwogse, J, Wu, K, Xiao, J, Yu, Z, Ronald, PC, Harrington, SE, Second, G, McCouch, SR and Tanksley, SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 12511374.
Cho, YC, Suh, JP, Choi, IS, Hong, HC, Baek, MK, Kang, KH, Kim, YG, Ahn, SN, Choi, HC, Hwang, HG and Moon, HP (2003) QTLs analysis of yield and its related traits in wild rice relative Oryza rufipogon . Treatises of Crop Research 4: 1929.
Fan, C, Xing, Y, Mao, H, Lu, T, Han, B, Xu, C, Li, X and Zhang, Q (2006) GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theoretical Applied Genetics 112: 11641171.
Ji, HS, Chu, SH, Jiang, W, Chi, YI, Hahn, JH, Eun, MY, McCouch, SR and Koh, HJ (2006) Characterization and mapping of a shattering mutant in rice that corresponds to a block of domestication genes. Genetics 173: 9951005.
Li, Y, Fan, C, Xing, Y, Jiang, Y, Luo, L, Sun, L, Shao, D, Xu, C, Li, X, Xiao, J, He, Y and Zhang, Q (2011) Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nature Genetics 43: 12661269.
Liu, T, Shao, D, Kovi, MR and Xing, Y (2010) Mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (Oryza sativa L.). Theoretical Applied Genetics 120: 933942.
Shomura, A, Izawa, T, Ebana, K, Ebitani, T, Kanegae, H, Konishi, S and Yano, M (2008) Deletion in a gene associated with grain size increased yields during rice domestication. Nature Genetics 40: 10231028.
Song, XJ, Huang, W, Shi, M, Zhu, MZ and Lin, HX (2007) A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nature Genetics 39: 623630.
Suh, J, Ahn, SN, Cho, YC, Kang, KH, Choi, IS, Kim, YG, Suh, HS and Hwang, HG (2005) Mapping for QTLs for yield traits using an advanced backcross population from a cross between Oryza sativa and O. glaberrima . Korean Journal Breeding Science 37: 214220.
Thomson, MJ, Tai, TH, McClung, AM, Lai, XH, Hinga, ME and Lobos, KB (2003) Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theoretical Applied Genetics 107: 479493.
Xie, XB, Jin, FX, Song, MH, Suh, JP, Hwang, HG, Kim, YG, McCouch, SR and Ahn, SN (2008) Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa× O. rufipogon cross. Theoretical Applied Genetics 116: 613622.
Yun, YT, Kim, DM, Park, IK, Chung, CT, Seong, YK and Ahn, SN (2010) Development and evaluation of QTL-NILs for grain weight from an interspecific cross in rice. Korean Journal Breeding Science 42: 357364.
Zhang, YS, Luo, LJ, Xu, CG, Zhang, QF and Xing, YZ (2006) Quantitative trait loci for panicle size, heading date and plant height co-segregating in trait-performance derived near-isogenic lines of rice (Oryza sativa). Theoretical Applied Genetics 113: 361368.
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Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
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