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Flexible filamentous virus structures from fiber diffraction

Published online by Cambridge University Press:  29 February 2012

Gerald Stubbs ,
Amy Kendall ,
Michele McDonald ,
Wen Bian ,
Timothy Bowles ,
Sarah Baumgarten ,
Ian McCullough ,
Jian Shi ,
Phoebe Stewart  and
Esther Bullitt
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Gerald Stubbs*
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Amy Kendall
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Michele McDonald
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Wen Bian
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Timothy Bowles
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Sarah Baumgarten
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Ian McCullough
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Jian Shi
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Phoebe Stewart
Affiliation:
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232
Esther Bullitt
Affiliation:
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118
David Gore
Affiliation:
BioCAT, Illinois Institute of Technology, Chicago, Illinois 60439
Said Ghabrial
Affiliation:
Plant Pathology Department, University of Kentucky, Lexington, Kentucky 40546
*
a)Author to whom correspondence should be addressed. Electronic mail: gerald.stubbs@vanderbilt.edu
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Abstract

Fiber diffraction data have been obtained from Narcissus mosaic virus, a potexvirus from the family Flexiviridae, and soybean mosaic virus (SMV), a potyvirus from the family Potyviridae. Analysis of the data in conjunction with cryo-electron microscopy data allowed us to determine the symmetry of the viruses and to make reconstructions of SMV at 19 Å resolution and of another potexvirus, papaya mosaic virus, at 18 Å resolution. These data include the first well-ordered data ever obtained for the potyviruses and the best-ordered data from the potexviruses, and offer the promise of eventual high resolution structure determinations.

Keywords

fiber diffractioncryo-electron microscopypotyviruspotexvirussoybean mosaic virus

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Type
X-Ray Diffraction
Information
Powder Diffraction , Volume 23 , Issue 2 , June 2008 , pp. 113 - 117
Copyright
Copyright © Cambridge University Press 2008

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