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The localization and assay of chemical elements by microprobe methods

Published online by Cambridge University Press:  17 March 2009

T. A. Hall  and
Brij L. Gupta
T. A. Hall
Affiliation:
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, U.K.
Brij L. Gupta
Affiliation:
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, U.K.
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Extract

The principle of the microprobe analysis of chemical elements is illustrated in Fig. i. Some kind of radiation is directed on to the specimen, generating signals characteristic of the elements present. Local analysis in situ is achieved in one of two ways. Most often the impinging beam is finely focused so that the signal at any moment comes only from a selected microregion. Alternatively, in some instruments, the impinging beam floods a larger region but the emergent signals characteristic of a particular element may be selected and focused to give an elemental ‘map’ or ‘image’.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 16 , Issue 3 , August 1983 , pp. 279 - 339
Copyright
Copyright © Cambridge University Press 1983

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