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Relaxation studies of ion transport systems in lipid bilayer membranes

Published online by Cambridge University Press:  17 March 2009

P. Läuger ,
R. Benz ,
G. Stark ,
E. Bamberg ,
P. C. Jordan ,
A. Fahr  and
W. Brock
P. Läuger
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
R. Benz
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
G. Stark
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
E. Bamberg
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
P. C. Jordan
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
A. Fahr
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
W. Brock
Affiliation:
Department of Biology, University of Konstanz, D-7750 Konstanz, Germany
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Extract

Relaxation techniques have been widely used in kinetic studies of chemical reactions in homogeneous solution (Eigen & DeMayer, 1963). The principle of this method is well known: an external variable such as temperature or pressure is suddenly changed and the time course of a state parameter of the system such as concentration is recorded as it approaches a new steady value. Relaxation techniques can also be used for studying the rate of elementary processes in membranes. This method has proved particularly useful for the investigation of ion transport systems (ion carriers, channels, pumps) in artificial planar bilayer membranes. In this review we describe different relaxation techniques which have been developed for this purpose during the last years, as well as applications to a number of ion transport systems.

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Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 14 , Issue 4 , November 1981 , pp. 513 - 598
Copyright
Copyright © Cambridge University Press 1981

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References

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