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Structures and mechanisms of DNA restriction and modification enzymes

Published online by Cambridge University Press:  17 March 2009

Paul Modrich
Paul Modrich
Affiliation:
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
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DNA restriction and modification enzymes are responsible for the hostspecific barriers to interstrain and interspecies transfer of genetic information that have been observed in a variety of bacterial cell types. Although the phenomenon of host specificity was initially observed in the early 1950s (Luria & Human, 1952; Bertani & Weigle, 1953), it was nearly a decade later that Arber and his colleagues accurately predicted the molecular basis of the phenomenon. Their experiments with bacteriophage λ demonstrated that a given host-specificity system imparts a specific modification to the viral DNA, and further, that restriction of DNA lacking the appropriate modification is s consquence of nucleolytic hydrolysis upon entry into the host cell (Arber & Dussoix, 1962; Dussoix & Arber, 1962; Arber, Hattman & Dussoix, 1963).

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Research Article
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Quarterly Reviews of Biophysics , Volume 12 , Issue 3 , August 1979 , pp. 315 - 369
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Copyright © Cambridge University Press 1979

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