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Feasibility of imaging living cells at subnanometer resolutions by ultrafast X-ray diffraction

  • Magnus Bergh (a1), Gösta Huldt (a1), Nicusor Tîmneanu (a1), Filipe R. N. C. Maia (a1) and Janos Hajdu (a1) (a2)...

Abstract

Detailed structural investigations on living cells are problematic because existing structural methods cannot reach high resolutions on non-reproducible objects. Illumination with an ultrashort and extremely bright X-ray pulse can outrun key damage processes over a very short period. This can be exploited to extend the diffraction signal to the highest possible resolution in flash diffraction experiments. Here we present an analysis of the interaction of a very intense and very short X-ray pulse with a living cell, using a non-equilibrium population kinetics plasma code with radiation transfer. Each element in the evolving plasma is modeled by numerous states to monitor changes in the atomic populations as a function of pulse length, wavelength, and fluence. The model treats photoionization, impact ionization, Auger decay, recombination, and inverse bremsstrahlung by solving rate equations in a self-consistent manner and describes hydrodynamic expansion through the ion sound speed. The results show that subnanometer resolutions could be reached on micron-sized cells in a diffraction-limited geometry at wavelengths between 0·75 and 1·5 nm and at fluences of 1011–1012 photons μm−2 in less than 10 fs. Subnanometer resolutions could also be achieved with harder X-rays at higher fluences. We discuss experimental and computational strategies to obtain depth information about the object in flash diffraction experiments.

Copyright

Corresponding author

*Author for correspondence: J. Hajdu, Laboratory of Molecular Biophysics, Institute of Cell and Molecular Biology, Uppsala University, Box 596, 75124 Uppsala, Sweden. Tel.:+46-18-4714449; Fax: +46-18-511755; Email: janos@xray.bmc.uu.se

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