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Microspectrophotometry of visual pigments

Published online by Cambridge University Press:  17 March 2009

Stanley D. Carlson
Stanley D. Carlson
Affiliation:
Department of Entomology, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
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Abstract

Visual pigments are embedded in the disc membranes of the outer segments of vertebrate rods and cones and in the microvilli of invertebrate visual cells. The pigment molecule in both is a most fascinating aggregate of known (the ubiquitous II-cis isomer of vitamin A1 or A2-aldehyde = retinal1 or 2; Hubbard & Wald, 1952) covalently bonded to the unknown (a protein termed opsin) (Anderson, Hoffman & Hall, 1971). This conjugated molecule is called rhodopsin or dehydrorhodopsin (porphryopsin) when the prosthetic portion is retinall or 2 respectively. So sensitive is this sterically hindered, bent and twisted molecule to light that absorption of one photon can initiate its isomerization to the all trans form. This conformational change is but one (but the best known) of the factors leading to receptor membrane changes ushering in the visual impulse.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 5 , Issue 3 , August 1972 , pp. 349 - 393
Copyright
Copyright © Cambridge University Press 1972

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