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A Novel Cellulose-Preparation Method

Published online by Cambridge University Press:  26 July 2018

Richard Gillespie
Richard Gillespie*
Affiliation:
Department of Archaeology and Natural History, School of Culture, History and Language, Australian National University, Acton ACT 2601, Australia; and Centre for Archaeological Science, School of Earth and Environmental Sciences, University of Wollongong, NSW 2522, Australia
*
*Corresponding author. Email: dizheights@gmail.com.
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Abstract

The radiocarbon (14C) dating of contaminated old wood has been seen as a challenge requiring many lengthy procedures, often using strong alkali extractions and carbon-containing solvents. Introduced here is a novel protocol called 2chlorOx, a twice-repeated sequence of alkaline hypochlorite and acidic chlorite oxidations, which is shown to work well for 14C and 13C measurements on both <5000 BP and >50,000 BP wood samples, producing results superior to those from conventional acidic chlorite or acidic dichromate oxidations. The 2chlorOx method employs only inorganic reagents, many samples can be completed in less than one day under normal laboratory conditions, and cellulose prepared in this way is usually paper-white in color.

Keywords

bleachingcellulosechloritehypochlorite

Information

Type
Research Article
Information
Radiocarbon , Volume 61 , Issue 1 , February 2019 , pp. 131 - 139
Copyright
© 2018 by the Arizona Board of Regents on behalf of the University of Arizona 

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References

REFERENCES

Anchukaitis, KJ, Evans, MN, Lange, T, Smith, DR, Leavitt, SW, Schrag, DP. 2008. Consequences of a rapid cellulose extraction technique for oxygen isotope and radiocarbon analyses. Analytical Chemistry 80:2035–2041.Google Scholar
Bird, MI, Ayliffe, LK, Fifield, LK, Cresswell, R, Turney, CSM, Barrows, TT, David, B. 1999. Radiocarbon dating of ‘old’ charcoal using a wet oxidation-stepped combustion procedure. Radiocarbon 41(1):127–140.Google Scholar
Chappell, J, Head, J, Magee, J. 1996. Beyond the radiocarbon limit in Australian archaeology and Quaternary research. Antiquity 70:543–552.Google Scholar
Cullen, LE, Grierson, PF. 2006. Is cellulose extraction necessary for developing stable carbon and oxygen isotopes chronologies from Callitris glaucophylla? Palaeogeography, Palaeoclimatology, Palaeoecology 236:206–216.Google Scholar
Fallon, SJ, Fifield, LK, Chappell, JM. 2010. The next chapter in radiocarbon dating at the Australian National University: Status report on the single stage AMS. Nuclear Instruments and Methods in Physics Research B 268:898–901.Google Scholar
Fifield, LK, Bird, MI, Turney, CSM, Hausladen, PA, Santos, GM, di Tada, ML. 2001. Radiocarbon dating of the human occupation of Australia prior to 40 ka B.P. – successes and pitfalls. Radiocarbon 43(3):1131–1145.Google Scholar
Fink, D, Hotchkis, M, Hua, Q, Jacobsen, G, Smith, AM, Zoppi, U, Child, D, Mifsud, C, van der Gaast, H, Williams, A, Williams, M. 2004. The ANTARES AMS facility at ANSTO. Nuclear Instruments and Methods in Physics Research B 223-224:109–115.Google Scholar
Garvey, CJ, Simon, GP, Parker, IH. 2005. On the interpretation of x-ray diffraction powder patterns in terms of the nanostructure of cellulose I fibres. Macromolecular Chemistry and Physics 206(15):1568–1575.Google Scholar
Garvey, CJ, Paris, O, Gillespie, R. 2007. X-ray diffraction as a tool to study the arrangement of cellulose molecules in plant cell walls. Proceedings of the Physiological Society. Abstracts for the 2007 Newcastle meeting, 38/173P.Google Scholar
Gaudinski, JB, Dawson, TE, Quideau, S, Schuur, EA, Roden, JS, Trumbore, SE, Sandquist, DR, Oh, S-W, Wasylishen, RE. 2005. Comparative analysis of cellulose preparation techniques for use with 13C, 14C, and 18O isotopic measurements. Analytical Chemistry 77:7212–7224.Google Scholar
Gillespie, R. 1997. Burnt and unburnt carbon: dating charcoal and burnt bone from the Willandra Lakes, Australia. Radiocarbon 39(3):239–250.Google Scholar
Gillespie, R. 1998. Alternative timescales: a critical review of Willandra Lakes dating. Archaeology in Oceania 33(3):169–182.Google Scholar
Gillespie, R, Fifield, LK, Levchenko, V, Wells, R. 2008. New 14C ages on cellulose from Diprotodon gut contents: explorations in oxidation chemistry and combustion. Radiocarbon 50(1):75–81.Google Scholar
Hajdas, I, Hendriks, L, Fontana, A, Monegato, G. 2017. Evaluation of preparation methods in radiocarbon dating of old wood. Radiocarbon 59(3):727–737.Google Scholar
Hatté, C, Morvan, J, Noury, C, Paterne, M. 2001. Is classical acid-alkali-acid treatment responsible for contamination? An alternative proposition. Radiocarbon 43(2A):177–182.Google Scholar
Hedges, REM, Bronk Ramsey, C, Van Klinken, G-J. 1998. An experiment to refute the likelihood of cellulose carboxylation. Radiocarbon 40(1):59–60.Google Scholar
Jull, AJT, Donahue, DJ, Damon, PE. 1996. Factors affecting the apparent radiocarbon age of textiles. Journal of Archaeological Science 23:157–160.Google Scholar
Kouznetsov, DA, Ivanov, AA, Valetsky, PR. 1996. Effects of fires and biofractionation of carbon isotopes on results of radiocarbon dating of old textiles: the Shroud of Turin. Journal of Archaeological Science 23:109–121.Google Scholar
Lawrie, KC, Brodie, RS, Tan, KP, Gibson, D, Magee, J, Clarke, JDA, Halas, L, Gow, L, Somerville, P, Apps, HE, Smith, M, Christensen, NB, Abraham, J, Hostetler, S, Brodie, RC. 2012. Geoscience Australia: Broken Hill Managed Aquifer Recharge Project. Report No. 2, 93-228.Google Scholar
Leavitt, SR, Danzer, SW. 1993. Method for batch processing small wood samples to holocellulose for stable-carbon isotope analysis. Analytical Chemistry 65:87–89.Google Scholar
Loader, NJ, Robertson, I, Barker, AC, Switzur, VR, Waterhouse, JS. 1997. An improved technique for the batch processing of small wholewood samples to α-cellulose. Chemical Geology 136:313–317.Google Scholar
McCormac, FG, Baillie, MGL, Pilcher, JR, Brown, DM, Hoper, ST. 1994. δ13C measurements from the Irish oak chronology. Radiocarbon 36(3):27–35.Google Scholar
Park, JH, Kim, JC, Cheoun, MK, Kim, Youn M, Liu, YH, Kim, ES. 2002. 14C level at Mt Chiak and Mt Kyeryong in Korea. Radiocarbon 44(2):559–566.Google Scholar
Santos, GM, Bird, MI, Pillans, B, Fifield, LK, Alloway, BV, Chappell, J. 2001. Radiocarbon dating of wood using different pretreatment procedures: application to the chronology of Rotoehu ash, New Zealand. Radiocarbon 43(1):239–248.Google Scholar
Scott, EM. 2003. The Fourth International Radiocarbon Intercomparison (FIRI); Section 6: Kauri Wood, Samples A and B. Radiocarbon 45(2):227–248.Google Scholar
Southon, JR, Magana, AL. 2010. A comparison of cellulose extraction and ABA pretreatment methods for AMS 14C dating of ancient wood. Radiocarbon 52(2-3):1371–1379.Google Scholar
Stevenson, J, Gillespie, R, Hope, G, Jacobsen, G, Fallon, S, Levchenko, V. 2010. The archaic and puzzling record of Lake Xere Wapo, New Caledonia. In: Haberle, S, Stevenson J, Prebble M, editors. Altered Ecologies: Fire, Climate and Human Influence on Terrestrial Landscapes. Terra Australis 32:381–393.Google Scholar
Stuiver, M, Polach, HA. 1977. Discussion: reporting of 14C data. Radiocarbon 19(3):355–363.Google Scholar
Switzur, R. 1989. Early English boats. Radiocarbon 31(3):1010–1018.Google Scholar
Wilson, AT, Grinsted, MJ. 1977. 12C/13C in cellulose and lignin as palaeothermometers. Nature 265:133–135.Google Scholar
Wissel, H, Mayr, C, Lücke, A. 2008. A new approach for the isolation of cellulose from aquatic plant tissue and freshwater sediments for stable isotope analysis. Organic Geochemistry 39:1545–1561.Google Scholar