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Accurate Age Estimation Using 14C Content in Japanese Human Teeth

  • Keisuke Kunita (a1), Toshio Nakamura (a1) and Kazuo Kato (a2)

We investigated age estimation of persons using 45 Japanese human teeth that consisted of mainly the third molar. We measured the radiocarbon content in tooth enamel hydroxyapatite from the crown as well as those in collagen and bioapatite in the root of the same tooth. Age estimations from tooth enamel apatite yielded an absolute age shift of 1.7±1.4 yr from the true age. This value suggests that estimating the age of Japanese people from tooth enamel is as accurate as the previous studies conducted in Europe and the USA. The 14C analysis of roots suggested that bioapatite is formed slightly later than collagen in the same tooth roots. The appropriate formation age ranges suggested by the tooth enamel analysis can be optimized and confirmed by using the additional F14C values of the root samples. We also noticed a characteristic trend of enamel δ13C values in comparison with the formation ages of the crowns of the teeth. The δ13C value tends to increase gradually from –13.0‰ to –11.5‰ from the 1960s to the 1970s, probably being caused by changes in the Japanese diet. This trend may support the selection of proper tooth formation age as supplemental data in addition to 14C content analyses.

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Selected Papers from the 2015 Radiocarbon Conference, Dakar, Senegal, 16–20 November 2015

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Alkass K, Buchholz BA, Ohtani S, Yamamoto T, Druid H, Spalding KL. 2010. Age estimation in forensic sciences: application of combined aspartic acid racemization and radiocarbon analysis. Molecular and Cellular Proteomics 9:10221030.
Alkass K, Buchholz BA, Druid H, Spalding KL. 2011. Analysis of 14C and 13C in teeth provides precise birth dating and clues to geographical origin. Forensic Science International 209:3441.
Alkass K, Satoh H, Buchholz BA, Bernard S, Holmlund G, Senn DR, Spalding KL, Druid H. 2013. Analysis of radiocarbon, stable isotopes and DNA in teeth to facilitate identification of unknown decedents. PLoS ONE 8(7):e69597.
Cook GT, Dunbar E, Black SM, Xu S. 2006. A preliminary assessment of age at death determination using the nuclear weapons testing 14C activity of dentine and enamel. Radiocarbon 48(3):305310.
Daito M, Sonomoto M, Kimura K, Mimura M, Katoh M, Daito M. 1997. Calcification of mandibular permanent molars. Syouni shigaku zasshi 35(1):96110. In Japanese with English abstract.
Eerkens JW, Berget AG, Bartelink EJ. 2011. Estimating weaning and early childhood diet serial micro-samples of dentin collagen. Journal of Archaeological Science 38(11):31013111.
Froehle AW, Kellner CM, Schoeninger MJ. 2010. FOCUS: effect of diet and protein source on carbon stable isotope ratios in collagen: follow up to Warinner and Tuross (2009). Journal of Archaeological Science 37(10):26622670.
Fuller BT, Fuller JL, Harris DA, Hedges REM. 2006. Detection of breastfeeding and weaning in modern human infants with carbon and nitrogen stable isotope ratios. American Journal of Physical Anthropology 129(2):279293.
Hua Q, Barbetti M. 2004. Review of tropospheric bomb 14C data for carbon cycle modeling and age calibration purposes. Radiocarbon 46(3):12731298.
Hua Q, Barbetti M. 2007. Influence of atmospheric circulation on regional 14CO2 differences. Journal of Geophysical Research 112:D19102.
Hua Q, Barbetti M, Rakowski AZ. 2013. Atmospheric radiocarbon for the period 1950–2010. Radiocarbon 55(4):20592072.
Kawanishi H. 1959. Statistical study on the third molar of Japanese - the statistical fluctuations on the timing of emergence, growth and eruption of the third molar. Journal of the Stomatological Society, Japan 26(2):463478. In Japanese.
Longin R. 1971. New method of collagen extraction for radiocarbon dating. Nature 230(5291):241242.
Minami M, Muto H, Nakamura T. 2004. Chemical techniques to extract organic fractions from fossil bones for accurate 14C dating. Nuclear Instruments and Methods in Physics Research B 223–224:302307.
Mook WG, van der Plicht J. 1999. Reporting 14C activities and concentrations. Radiocarbon 41(3):227239.
Nakamura T, Niu E, Oda H, Ikeda A, Minami M, Ohta T, Oda T. 2004. High precision 14C measurements with the HVEE Tandetron AMS system at Nagoya University. Nuclear Instruments and Methods in Physics Research B 223–224:124129.
Nakamura T, Nishida I, Takada H, Okuno M, Minami M, Oda H. 2007. Marine reservoir effect deduced from 14C dates on marine shells and terrestrial remains at archeological sites in Japan. Nuclear Instruments and Methods in Physics Research B 259:453459.
Nishihara G, Daito M. 1989. Clinical observations on the stages of tooth formation – especially permanent incisors. Shika Igaku (Journal of Osaka Odontological Society) 52(6):771810. In Japanese with abstract in English.
Nolla CM. 1960. The development of the permanent teeth. Journal of Dentistry for Children 27:254263.
Reimer PJ, Brown TA, Reimer RW. 2004. Discussion: reporting and calibration of post-bomb 14C data. Radiocarbon 46(3):12991304.
Smith BH. 1991. Standards of human tooth formation and dental age assessment. In: Kelley MA, Larsen CS, editors. Advances in Dental Anthropology. New York: Wiley-Liss. p 143168.
Spalding KL, Buchholz BA, Bergman LE, Druid H, Frisén J. 2005. Forensics: age written in teeth by nuclear tests. Nature 437(7057):333334.
Stuiver M, Polach HA. 1977. Discussion: reporting of 14C data. Radiocarbon 19(3):355363.
Sullivan CH, Krueger HW. 1981. Carbon isotope analysis of separate chemical phases in modern and fossil bone. Nature 292(5281):333335.
Tanaka M. 1988. Clinical observations on the stages of tooth formation—especially permanent molars. Shika Igaku (Journal of Osaka Odontological Society) 51(4):739800. In Japanese with abstract in English.
Tanaka T, Daito M. 1992. Clinical research on the development of third molars in panoramamic radiographs. Shika Igaku (Journal of Osaka Odontological Society) 55(4):337348. In Japanese with English abstract.
van der Sluis LG, Reimer OJ, Lynnerup N. 2015. Investigating intra-individual dietary change and 14C ages using high-resolution δ13C and δ15N isotope ratios and 14C ages obtained from dentine increments. Radiocarbon 57(4):665677.
Wang N, Shen CD, Ding P, Yi WX, Sun WD, Liu KX, Ding XF, Fu DP, Yuan J, Yang XY, Zhou LP. 2010. Improved application of bomb carbon in teeth for forensic investigation. Radiocarbon 52(2):706716.
Warinner C, Tuross N. 2009. Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications. Journal of Archaeological Science 36(8):16901697.
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