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Collagen Extraction and Stable Isotope Analysis of Small Vertebrate Bones: A Comparative Approach

  • S Cersoy (a1), A Zazzo (a1), M Lebon (a2), J Rofes (a1) and S Zirah (a3)...
Abstract
Abstract

Bone remains of small vertebrate fossils provide valuable information for paleoenvironmental and paleoclimatic reconstructions. However, direct radiocarbon dating of small vertebrates remains challenging as the extraction of sufficient good quality collagen is required. The efficiency of eight collagen extraction protocols was tested on seven samples, representative of different ages and burial environments, including both macro and small vertebrate taxa. First, the samples were prescreened using attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR) to quantify collagen content in archaeological bones, revealing that one should be discarded for 14C dating. Then, the quantity of protein extracted (yield) and collagen integrity were checked using conventional elemental analysis. The results show that one protocol was not able to accurately extract collagen from the samples. A soft HCl-based protocol seems more appropriate for the pretreatment of archaeological small mammal bones, whereas a harsher protocol might be more efficient to extract a higher amount of collagen from large mammals as well as amphibian bones. The influence of the tested protocols on carbon and nitrogen isotope values was also investigated. The results showed that isotopic variability, when existing, is related to the interindividual differences rather than the different protocols.

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*Corresponding author. Email: sophie.cersoy@mnhn.fr.
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Selected Papers from the 2015 Radiocarbon Conference, Dakar, Senegal, 16–20 November 2015

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This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

KA Arslanov , YS Svezhentsev . 1993. An improved method for radiocarbon dating fossil bones. Radiocarbon 35(3):387391.

F Brock , R Wood , TFG Higham , P Ditchfield , A Bayliss , C Bronk Ramsey . 2012. Reliability of nitrogen content (%N) and carbon: nitrogen atomic ratios (C:N) as indicators of collagen preservation suitable for radiocarbon dating. Radiocarbon 54(3–4):879886.

I Caputo , M Lepretti , C Scarabino , C Esposito , A Proto . 2012. An acetic acid-based extraction method to obtain high quality collagen from archaeological bone remains. Analytical Biochemistry 421(1):9296.

MJ DeNiro . 1985. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317(6040):806809.

B Farre , P Massard , J Nouet , Y Dauphin . 2014. Preservation of rodent bones from El Harhoura 2 cave (Morocco, Neolithic – Middle Palaeolithic): microstructure, mineralogy, crystallinity and composition. Journal of African Earth Sciences 92:113.

EM Keaveney , PJ Reimer . 2012. Understanding the variability in freshwater radiocarbon reservoir offsets: a cautionary tale. Journal of Archaeological Science 39(5):13061316.

A Royer . 2016. How complex is the evolution of small mammal communities during the Late Glacial in southwest France? Quaternary International. doi: 10.1016/j.quaint.2015.12.065.

HE Suess . 1955. Radiocarbon concentration in modern wood. Science 122(3166):415417.

MR Waters , TW Stafford , B Kooyman , LV Hills . 2015. Late Pleistocene horse and camel hunting at the southern margin of the ice-free corridor: reassessing the age of Wally’s Beach, Canada. Proceedings of the National Academy of Sciences of the USA 112(14):42634267.

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Radiocarbon
  • ISSN: 0033-8222
  • EISSN: 1945-5755
  • URL: /core/journals/radiocarbon
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