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Contemporary Fraction of bis(2–ethylhexyl) Phthalate in Stilton Cheese by Accelerator Mass Spectrometry

Published online by Cambridge University Press:  09 February 2016

Michael A Nelson ,
John M Ondov ,
Michael C VanDerveer  and
Bruce A Buchholz
Michael A Nelson*
Affiliation:
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA Analytical Chemistry Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
John M Ondov
Affiliation:
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA
Michael C VanDerveer
Affiliation:
US Food and Drug Administration, University Station, College Park, Maryland 20740 USA
Bruce A Buchholz
Affiliation:
Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, California 94551, USA
*
Corresponding author. Email: manelson@umd.edu.
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Abstract

Measurements of the radiocarbon abundance in 5 samples of bis(2–ethylhexyl) phthalate (DEHP) isolated from Stilton cheese were made by accelerator mass spectrometry (AMS) to determine the fraction of carbon originating from contemporary biogenic sources. DEHP is classified as a “priority hazardous substance” by the European Union, a probable human carcinogen by the United States Environmental Protection Agency, and is suspected to be a human endocrine disrupter. Measurement of its 14C abundance in a specific food indicates whether its presence is due to contamination from industrially synthesized DEHP or a naturally inherent component. A method was developed to determine the contemporary carbon fraction of DEHP in a fatty food matrix at concentrations of 0.14 mg/kg. Five 90–μg quantities of DEHP were extracted from 12 kg of Stilton cheese and isolated by silica gel, size exclusion, and high-performance liquid chromatography (HPLC). Masses of samples were determined by gas chromatography mass spectrometry (GC-MS) analyses prior to combustion and manometry afterwards. The purity of DEHP carbon mass in each isolate was determined by multivariate deconvolution of GCMS fragmentation spectra obtained from measurements of standards and isolates, and ranged from 88.0 ± 1.8% to 92.3 ± 1.1% (n = 5, 1σ). Concurrently processed isolation method blanks contained from 0.15 ± 0.04 to 1.52 ± 0.06 μg (n = 3, 1σ) DEHP per sample and significant quantities of pre- and post-chromatographic extraneous carbon contamination. The mean 14C-corrected contemporary carbon fraction of DEHP in the isolates was 0.235 ± 0.073 (1σ; and ± 0.091 at the 95% confidence level), revealing that the majority of DEHP in Stilton cheese results from anthropogenic sources, but with a significant naturally occurring component.

Type
Articles
Information
Radiocarbon , Volume 55 , Issue 2: Proceedings of the 21st International Radiocarbon Conference (Part 1 of 2) , 2013 , pp. 686 - 697
Copyright
Copyright © 2013 by the Arizona Board of Regents on behalf of the University of Arizona 

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References

Amade, P, Mallea, M, Bouaicha, N. 1994. Isolation, structural identification and biological activity of two metabolites produced by Penicillium Olsonii Bainier and Sartory. Journal of Antibiotics 47(2):201–7.Google Scholar
Brown, TA, Southon, JR. 1997. Corrections for contamination background in AMS 14C measurements. Nuclear Instruments and Methods in Physics Research B 123(1–4):208–13.CrossRefGoogle Scholar
Castle, L, Gilbert, J, Eklund, T. 1990. Migration of plasticizer from poly(vinyl chloride) milk tubing. Food Additives and Contaminants 7(5):591–6.CrossRefGoogle ScholarPubMed
Chen, CY. 2004. Biosynthesis of di-92–ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) from red alga - Bangia atropurpurea. Water Research 38(4): 1014–8.CrossRefGoogle ScholarPubMed
David, R. 2000. Human exposure estimates for phthalates: correspondence. Environmental Health Perspectives 108(10):A440–A442.Google Scholar
Enneking, PA. 2006. Phthalates not in plastic food packaging. Environmental Health Perspectives 114(2): A89–A90.Google Scholar
European Council for Plasticisers and Intermediates (ECPI). 2012. DEHP Information Centre: About DEHP [Internet]. Accessed 13 September 2012. URL: http://www.dehp-facts.com/DEHP.Google Scholar
Grubbs, FE. 1969. Procedures for detecting outlying observations in samples. Technometrics 11(1): 1–21.CrossRefGoogle Scholar
Jarosova, A. 2006. Phthalic acid esters (PAEs) in the food chain. Czech Journal of Food Sciences 24(5):223–31.Google Scholar
Namikoshi, M, Fujiwarea, T, Nishikawa, T, Ukai, K. 2006. Natural abundance 14C content of dibutyl phthalate (DBP) from three marine algae. Marine Drugs 4(4): 290–7.Google Scholar
Pearson, A, McNichol, AP, Benitez-Nelson, BC, Hayes, JM, Eglinton, TI. 2001. Origins of lipid biomarkers in Santa Monica Basin surface sediment: a case study using compound specific 14C analysis. Geochimica et Cosmochimica Acta 65(18):3123–37.CrossRefGoogle Scholar
Reddy, CM, Pearson, A, Xu, L, McNichol, AP, Bennter, BA Jr, Wiste, SA, Klouda, GA, Currie, LA, Eglinton, TI. 2002. Radiocarbon as a tool to apportion the sources of polycyclic aromatic hydrocarbons and black carbon in environmental samples. Environmental Science & Technology 36(8): 1774–82.CrossRefGoogle ScholarPubMed
Reimer, PJ, Brown, TA, Reimer, RW. 2004. Discussion: reporting and calibration of post-bomb 14C data. Radiocarbon 46(3): 1299–304.Google Scholar
Santos, GM, Southon, JR, Druffel-Rodriguez, KC, Griffin, S, Mazon, M. 2004. Magnesium perchlorate as an alternative water trap in AMS graphite sample preparation: a report on sample preparation at KCCAMS at the University of California, Irvine. Radiocarbon 46(1): 165–73.Google Scholar
Schettler, T. 2006. Human exposure to phthalates via consumer products. International Journal of Andrology 29(1):134–5.Google Scholar
Stuiver, M, Polach, HA. 1977. Discussion: reporting of 14C data. Radiocarbon 19(3):355–63.Google Scholar
US Environmental Protection Agency (US EPA). 1997. Integrated Risk Information System Database: Di(2– ethylhexyl)phthalate (DEHP) [Internet]. CASRN 117–81–7. Accessed 14 September 2012. URL: http://www.epa.gov/IRIS/subst/0014.htm.Google Scholar
Vogel, JS, Southon, JR, Nelson, DE. 1987. Catalyst and binder effects in the use of filamentous graphite for AMS. Nuclear Instruments and Methods in Physics Research B 29(1–2):50–6.CrossRefGoogle Scholar
Wenzl, T. 2009. Methods for the Determination of Phthalates in Food. JRC Scientific and Technical Reports, Institute for Reference Materials and Measurements, Joint Research Centre, European Commission, EUR23682 EN, Luxembourg.Google Scholar
Wormuth, M, Scheringer, M, Vollenweider, M, Hungerbuhler, K. 2006. What are the sources of exposure to eight frequently used phthalic acid esters in Europeans? Risk Analysis 26(3):803–24.Google Scholar
Zhu, J, Phillips, SP, Feng, YL, Yang, X. 2006. Phthalate esters in human milk: concentration variations over a 6 month postpartum time. Environmental Science & Technology 40(17): 5276–81.Google Scholar