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Histone Adduction with Nicotine: A Bio-Ams Study

Published online by Cambridge University Press:  18 July 2016

X. H. Wu ,
H. F. Wang ,
Y. F. Liu ,
X. Y. Lu ,
J. J. Wang  and
K. Li
X. H. Wu
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
H. F. Wang
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
Y. F. Liu
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
X. Y. Lu
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
J. J. Wang
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
K. Li
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
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Abstract

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Based on the study of DNA adduction with nicotine, we have measured the mouse hepatic histone adduction with 14C-labeled nicotine in vivo by bio-accelerator mass spectrometry (bio-AMS). In the exposure of mice to nicotine, the dose range administered was from 0.2 μg to 6.0 μg kg b.w.-1, which was equivalent to a very low level of human exposure to cigarette smoke. The adducts of either histone 1 (H1) or histone 3 (H3) with nicotine in mouse liver increased markedly with increasing nicotine dose. Our results have demonstrated that in the study of protein adduction with toxic xenobiotics as a biomarker, the AMS method achieves the highest sensitivity, 4.6 × 10-17 mol (46 amol) adducts per mg H1 protein, compared to all the other methods used previously.

Type
Research Article
Information
Radiocarbon , Volume 39 , Issue 3 , 1997 , pp. 293 - 297
Copyright
Copyright © The American Journal of Science 

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