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Using Stable Carbon Isotopes to Quantify Radiocarbon Reservoir Age Offsets in the Coastal Black Sea

  • Guillaume Soulet (a1) (a2), Liviu Giosan (a1), Clément Flaux (a3) and Valier Galy (a4)


Constraining radiocarbon (14C) reservoir age offsets is critical to deriving accurate calendar-age chronologies from 14C dating of materials which did not draw carbon directly from the atmosphere. The application of 14C dating to such materials is severely limited in hydrologically sensitive environments like the Black Sea because of the difficulty to quantify reservoir age offsets, which can vary quickly and significantly through time, due to the dynamics of the biogeochemical cycling of carbon. Here we reconstruct 14C reservoir age offsets (Rshell-atm) of Holocene bivalve shells from the coastal Black Sea relatively to their contemporaneous atmosphere. We show that the 14C reservoir age offset and the stable carbon isotope composition of bivalve shells are linearly correlated in this region. From a biogeochemical standpoint, this suggests that inorganic stable carbon isotope and 14C compositions of Black Sea coastal waters are controlled by the balance between autochthonous primary productivity and heterotrophic respiration of allochthonous pre-aged terrestrial organic matter supplied by rivers. This provided an important implication for Black Sea geochronology as the reservoir age offset of 14C-dated bivalve shell can be inferred from its stable carbon isotope composition. Our results provide a fundamental and inexpensive geochemical tool which will considerably improve the accuracy of Holocene calendar age chronologies in the Black Sea.


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Aksu, AE, Hiscott, RN, Mudie, PJ, Rochon, A, Kaminski, MA, Abrajano, T, Yaşar, D. 2002. Persistent Holocene outflow from the Black Sea to the Eastern Mediterranean Contradicts Noah’s Flood Hypothesis. GSA Today 12(5):410.
Ascough, PL, Cook, GT, Dugmore, AJ. 2005. Methodological approaches to determining the marine radiocarbon reservoir effect. Progress in Physical Geography 29(4):532547.
Ascough, PL, Church, MJ, Cook, GT, Dunbar, E, Gestsdóttir, H, McGovern, TH, Dugmore, AJ, Fridriksson, A, Edwards, KJ. 2012. Radiocarbon reservoir effects in human bone collagen from northern Iceland. Journal of Archaeological Science 39(7):22612271.
Badertscher, S, Fleitmann, D, Cheng, H, Edwards, RL, Göktürk, OM, Zumbühl, A, Leuenberger, M, Tüysüz, O. 2011. Pleistocene water intrusions from the Mediterranean and Caspian seas into the Black Sea. Nature Geoscience 4(4):236239.
Bahr, A, Arz, HW, Lamy, F, Wefer, G. 2006. Late glacial to Holocene paleoenvironmental evolution of the Black Sea, reconstructed with stable oxygen isotope records obtained on ostracod shells. Earth and Planetary Science Letters 241(3–4):863875.
Bondevik, S, Mangerud, J, Birks, HH, Gulliksen, S, Reimer, PJ. 2006. Changes in North Atlantic radiocarbon reservoir ages during the Allerod and Younger Dryas. Science 312(5779):15141517.
Bronk Ramsey, C, Schulting, R, Goriunova, OI, Bazaliiskii, VI, Weber, AW. 2014. Analyzing radiocarbon reservoir offsets through stable nitrogen isotopes and Bayesian modeling: A case study using paired human and faunal remains from the Cis-Baikal region, Siberia. Radiocarbon 56(2):789799.
Calmels, D, Gaillardet, J, Brenot, A, France-Lanord, C. 2007. Sustained sulfide oxidation by physical erosion processes in the Mackenzie River basin: Climatic perspectives. Geology 35(11):1003.
Coolen, MJL, Orsi, WD, Balkema, C, Quince, C, Harris, K, Sylva, SP, Filipova-Marinova, M, Giosan, L. 2013. Evolution of the plankton paleome in the Black Sea from the Deglacial to Anthropocene. Proceedings of the National Academy of Sciences 110(21):86098614.
Deuser, WG. 1970. Isotopic evidence for diminishing supply of available carbon during diatom bloom in the Black Sea. Nature 225(5237):10691071.
Dewar, G, Pfeiffer, S. 2010. Approaches to estimating marine protein in human collagen for radiocarbon date calibration. Radiocarbon 52(4):16111625.
Flaux, C, Rouchet, P, Popova, T, Sternberg, M, Guibal, F, Talon, B, Baralis, A, Panayotova, K, Morhange, C, Riapov, AV. 2016. An Early Bronze Age pile-dwelling settlement discovered in Alepu lagoon (municipality of Sozopol, department of Burgas), Bulgaria. Méditerranée 126:5770.
Fontugne, M, Guichard, F, Bentaleb, I, Strechie, C, Lericolais, G. 2009. Variations in 14C reservoir ages of Black Sea waters and sedimentary organic carbon during anoxic periods: Influence of photosynthetic versus chemoautotrophic production. Radiocarbon 51(3):969976.
Giosan, L, Coolen, MJL, Kaplan, JO, Constantinescu, S, Filip, F, Filipova-Marinova, M, Kettner, AJ, Thom, N. 2012. Early anthropogenic transformation of the Danube-Black Sea system. Scientific Reports 2(1):582.
Giosan, L, Filip, F, Constatinescu, S. 2009. Was the Black Sea catastrophically flooded in the early Holocene? Quaternary Science Reviews 28(1–2):16.
Hollander, DJ, McKenzie, JA. 1991. CO2 control on carbon-isotope fractionation during aqueous photosynthesis: A paleo-pCO2 barometer. Geology 19(9):929932.
Jones, GA, Gagnon, AR. 1994. Radiocarbon chronology of Black Sea sediments. Deep Sea Research Part I: Oceanographic Research Papers 41(3):531557.
Jull, AJT, Burr, GS, Hodgins, GWL. 2013. Radiocarbon dating, reservoir effects, and calibration. Quaternary International. 299:6471.
Karatayev, AY, Burlakova, LE, Padilla, DK. 2006. Growth rate and longevity of Dreissena polymorpha (Pallas): A review and recommendations for future study. Journal of Shellfish Research 25(1):2332.
Kessler, JD, Reeburgh, WS, Southon, J, Seifert, R, Michaelis, W, Tyler, SC. 2006. Basin-wide estimates of the input of methane from seeps and clathrates to the Black Sea. Earth and Planetary Science Letters 243(3–4):366375.
Kusch, S, Rethemeyer, J, Hopmans, EC, Wacker, L, Mollenhauer, G. 2016. Factors influencing 14C concentrations of algal and archaeal lipids and their associated sea surface temperature proxies in the Black Sea. Geochimica et Cosmochimica Acta 188:3557.
Kusch, S, Rethemeyer, J, Schefuß, E, Mollenhauer, G. 2010. Controls on the age of vascular plant biomarkers in Black Sea sediments. Geochimica et Cosmochimica Acta 74(24):70317047.
Kuzmin, YV, Nevesskaya, LA, Krivonogov, SK, Burr, GS. 2007. Apparent 14C ages of the “pre-bomb” shells and correction values (R, ΔR) for Caspian and Aral Seas (Central Asia). Nuclear Instruments and Methods in Physics Research B 259(1):463466.
Kwiecien, O, Arz, HW, Lamy, F, Plessen, B, Bahr, A, Haug, GH. 2009. North Atlantic control on precipitation pattern in the eastern Mediterranean/Black Sea region during the last glacial. Quaternary Research 71(3):375384.
Kwiecien, O, Arz, HW, Lamy, F, Wulf, S, Bahr, A, Röhl, U, Haug, GH. 2008. Estimated reservoir ages of the Black Sea since the Last Glacial. Radiocarbon 50(1):99118.
Leng, MJ, Marshall, JD. 2004. Palaeoclimate interpretation of stable isotope data from lake sediment archives. Quaternary Science Reviews 23(7–8):811831.
Li, Y, Qiang, M, Jin, Y, Liu, L, Zhou, A, Zhang, J. 2017. Influence of aquatic plant photosynthesis on the reservoir effect of Genggahai Lake, Northeastern Qinghai-Tibetan Plateau. Radiocarbon in press doi:10.1017/RDC.2017.127.
Lougheed, BC, van der Lubbe, HJL, Davies, GR. 2016. 87Sr/86Sr as a quantitative geochemical proxy for 14C reservoir age in dynamic, brackish waters: Assessing applicability and quantifying uncertainties. Geophysical Research Letters 43(2):735742.
Major, CO, Goldstein, SL, Ryan, WBF, Lericolais, G, Piotrowski, AM, Hajdas, I. 2006. The co-evolution of Black Sea level and composition through the last deglaciation and its paleoclimatic significance. Quaternary Science Reviews 25(17–18):20312047.
McCallister, SL, del Giorgio, PA. 2012. Evidence for the respiration of ancient terrestrial organic C in northern temperate lakes and streams. Proceedings of the National Academy of Sciences 109(42):1696316968.
McNichol, AP, Osborne, EA, Gagnon, AR, Fry, B, Jones, GA. 1994. TIC, TOC, DIC, DOC, PIC, POC—unique aspects in the preparation of oceanographic samples for 14C-AMS. Nuclear Instruments and Methods in Physics Research B 92(1–4):162165.
Mook, WG, Bommerson, JC, Staverman, WH. 1974. Carbon isotope fractionation between dissolved bicarbonate and gaseous carbon dioxide. Earth and Planetary Science Letters 22(2):169176.
Mook, WG, van der Plicht, J. 1999. Reporting 14C activities and concentrations. Radiocarbon 41(3):227239.
Okaniwa, N, Miyaji, T, Sasaki, T, Tanabe, K. 2010. Shell growth and reproductive cycle of the Mediterranean mussel Mytilus galloprovincialis in Tokyo Bay, Japan: Relationship with environmental conditions. Plankton and Benthos Research 5(Supplement):214220.
Olsen, J, Heinemeier, J, Lübke, H, Lüth, F, Terberger, T. 2010. Dietary habits and freshwater reservoir effects in bones from a Neolithic NE German cemetery. Radiocarbon 52(2):635644.
Özsoy, E, Ünlüata, Ü. 1997. Oceanography of the Black Sea: A review of some recent results. Earth-Science Reviews 42(4):231272.
Reimer, PJ, Bard, E, Bayliss, A, Beck, JW, Blackwell, PG, Bronk Ramsey, C, Buck, CE, Cheng, H, Edwards, RL, Friedrich, M, Grootes, PM, Guilderson, TP, Haflidason, H, Hajdas, I, Hatté, C, Heaton, TJ, Hoffmann, DL, Hogg, AG, Hughen, KA, Kaiser, KF, Kromer, B, Manning, SW, Niu, M, Reimer, RW, Richards, DA, Scott, EM, Southon, JR, Staff, RA, Turney, CSM, van der Plicht, J. 2013. IntCal13 and Marine13 radiocarbon age calibration curves 0–50,000 years cal BP. Radiocarbon 55(4):18691887.
Reimer, PJ, Brown, TA, Reimer, RW. 2004. Discussion: Reporting and calibration of post-bomb 14C data. Radiocarbon 46(3):12991304.
Romanek, CS, Grossman, EL, Morse, JW. 1992. Carbon isotopic fractionation in synthetic aragonite and calcite: Effects of temperature and precipitation rate. Geochimica et Cosmochimica Acta 56(1):419430.
Ross, DA, Degens, ET, MacIlvaine, J. 1970. Black Sea: Recent Sedimentary History. Science 170(3954):163165.
Ryan, WBF, Pitman, WC, Major, CO, Shimkus, K, Moscalenko, V, Jones, GA, Dimitrov, P, Gorür, N, Sakinç, M, Yüce, H. 1997. An abrupt drowning of the Black Sea shelf. Marine Geology 138(1–2):119126.
Santos, GM, Southon, JR, Griffin, S, Beaupre, SR, Druffel, ERM. 2007. Ultra small-mass AMS 14C sample preparation and analyses at KCCAMS/UCI Facility. Nuclear Instruments and Methods in Physics Research B 259(1):293302.
Sayle, KL, Hamilton, WD, Gestsdóttir, H, Cook, GT. 2016. Modelling Lake Mývatn’s freshwater reservoir effect: Utilisation of the statistical program FRUITS to assist in the re-interpretation of radiocarbon dates from a cemetery at Hofstaðir, north-east Iceland. Quaternary Geochronology 36:111.
Schmitt, J, Schneider, R, Elsig, J, Leuenberger, D, Lourantou, A, Chappellaz, J, Kohler, P, Joos, F, Stocker, TF, Leuenberger, M, Fischer, H. 2012. Carbon isotope constraints on the deglacial CO2 rise from ice cores. Science 336(6082):711714.
Schoeninger, M, DeNiro, M, Tauber, H. 1983. Stable nitrogen isotope ratios of bone collagen reflect marine and terrestrial components of prehistoric human diet. Science 220(4604):13811383.
Schoeninger, MJ, DeNiro, MJ. 1984. Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochimica et Cosmochimica Acta 48(4):625639.
Schulting, RJ, Ramsey, CB, Bazaliiskii, VI, Goriunova, OI, Weber, A. 2014. Freshwater reservoir offsets investigated through paired human-faunal 14C dating and stable carbon and nitrogen isotope analysis at Lake Baikal, Siberia. Radiocarbon 56(3):9911008.
Siani, G, Paterne, M, Arnold, M, Bard, E, Métivier, B, Tisnerat, N, Bassinot, F. 2000. Radiocarbon reservoir ages in the Mediterranean Sea and Black Sea. Radiocarbon 42(2):271280.
Siani, G, Paterne, M, Michel, E, Sulpizio, R, Sbrana, A, Arnold, M, Haddad, G. 2001. Mediterranean sea surface radiocarbon reservoir age changes since the Last Glacial Maximum. Science 294(5548):19171920.
Soulet, G. 2015. Methods and codes for reservoir–atmosphere 14C age offset calculations. Quaternary Geochronology 29:97103.
Soulet, G, Menot, G, Bayon, G, Rostek, F, Ponzevera, E, Toucanne, S, Lericolais, G, Bard, E. 2013. Abrupt drainage cycles of the Fennoscandian Ice Sheet. Proceedings of the National Academy of Sciences 110(17):66826687.
Soulet, G, Ménot, G, Garreta, V, Rostek, F, Zaragosi, S, Lericolais, G, Bard, E. 2011a. Black Sea “Lake” reservoir age evolution since the Last Glacial—Hydrologic and climatic implications. Earth and Planetary Science Letters 308(1–2):245258.
Soulet, G, Ménot, G, Lericolais, G, Bard, E. 2011b. A revised calendar age for the last reconnection of the Black Sea to the global ocean. Quaternary Science Reviews 30(9–10):10191026.
Soulet, G, Skinner, LC, Beaupré, SR, Galy, V. 2016. A note on reporting of reservoir 14C disequilibria and age offsets. Radiocarbon 58(1):205211.
Stuiver, M, Polach, HA. 1977. Discussion: Reporting of 14C data. Radiocarbon 19(3):355363.
van der Meer, MTJ, Sangiorgi, F, Baas, M, Brinkhuis, H, Sinninghe Damsté, JS, Schouten, S. 2008. Molecular isotopic and dinoflagellate evidence for Late Holocene freshening of the Black Sea. Earth and Planetary Science Letters 267(3–4):426434.
Wood, RE, Higham, TFG, Buzilhova, A, Suvorov, A, Heinemeier, J, Olsen, J. 2013. Freshwater radiocarbon reservoir effects at the burial ground of Minino, northwest Russia. Radiocarbon 55(1):163177.
Yanko-Hombach, V, Mudie, PJ, Kadurin, S, Larchenkov, E. 2014. Holocene marine transgression in the Black Sea: New evidence from the northwestern Black Sea shelf. Quaternary International 345:100118.


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Using Stable Carbon Isotopes to Quantify Radiocarbon Reservoir Age Offsets in the Coastal Black Sea

  • Guillaume Soulet (a1) (a2), Liviu Giosan (a1), Clément Flaux (a3) and Valier Galy (a4)


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