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On the nature and timing of oxygen radical production following exposure of Arabidopsis thaliana leaves to uranium, cadmium or a combination of both stressors

Published online by Cambridge University Press:  09 January 2012

J. Barescut ,
D. Lariviere ,
T. Stocki ,
N. Horemans ,
N. Vanhoudt ,
M. Janssens ,
B. Van Chaze ,
J. Wannijn ,
M. Van Hees  and
H. Vandenhove
N. Horemans
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
N. Vanhoudt
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
M. Janssens
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
B. Van Chaze
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
J. Wannijn
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
M. Van Hees
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
H. Vandenhove
Affiliation:
Belgian Nuclear Research Centre (SCKCEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium
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Abstract

The toxicity and oxidative stress responses of 19-day old Arabidopsis seedlings induced by U (66 μM) and Cd (20 μM) alone or in a binary mixture set-up (equitoxic mixture) are studied in function of time. After 48h a significant decrease in root and shoot growth and a simultaneous increase in anthocyanin production was evident in all treated plants.

Production of O−2 or H2O2 was visualized by staining freshly harvested leaves with nitrobluetetrazolium or diaminobezidine, respectively. With this method production of O−2 was only significantly measurable after 168 h treatment which coincides with a significant decrease in biomass production and probably also plant cell death. For Cd treated plants a significant increase in H2O2 production was measurable from 24h onwards. In contrast, a similar H2O2 production could not be measured in U or U + Cd treated plants. Both water and lipophilic soluble antioxidants significantly increased in U treated plants after 48 h. These high antioxidant levels might detoxify potential H2O2 produced in the U treated plants. In contrast for Cd treated plants only after 168h a significant increase in water soluble antioxidants was measured whereas no difference in the lipophilic fraction was visible.

Type
Research Article
Information
Radioprotection , Volume 46 , Issue 6: ICRER 2011 – International Conference on Radioecology & Environmental Radioactivity: Environment & Nuclear Renaissance , 2011 , pp. S491 - S496
Copyright
© Owned by the authors, published by EDP Sciences, 2011

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