Hostname: page-component-76fb5796d-vfjqv Total loading time: 0 Render date: 2024-04-29T08:51:38.823Z Has data issue: false hasContentIssue false
  • English
  • Français

Study of biological effects and oxidative stress related responses in gamma irradiated Arabidopsis thalianaplants

Published online by Cambridge University Press:  09 January 2012

J. Barescut ,
D. Lariviere ,
T. Stocki ,
N. Vanhoudt ,
A. Cuypers ,
J. Vangronsveld ,
N. Horemans ,
J. Wannijn ,
M. Van Hees  and
H. Vandenhove
N. Vanhoudt
Affiliation:
Belgian Nuclear Research Centre, Biosphere Impact Studies, 2400 Mol, Belgium
A. Cuypers
Affiliation:
Hasselt University, Centre for Environmental Sciences, 3590 Diepenbeek, Belgium
J. Vangronsveld
Affiliation:
Hasselt University, Centre for Environmental Sciences, 3590 Diepenbeek, Belgium
N. Horemans
Affiliation:
Belgian Nuclear Research Centre, Biosphere Impact Studies, 2400 Mol, Belgium
J. Wannijn
Affiliation:
Belgian Nuclear Research Centre, Biosphere Impact Studies, 2400 Mol, Belgium
M. Van Hees
Affiliation:
Belgian Nuclear Research Centre, Biosphere Impact Studies, 2400 Mol, Belgium
H. Vandenhove
Affiliation:
Belgian Nuclear Research Centre, Biosphere Impact Studies, 2400 Mol, Belgium
Get access

Abstract

This study aimed at investigating biological effects in Arabidopsis thalianaleaves and roots irradiated for 72 h with 3.5 Gy or 30 Gy of gamma radiation, and to unravel oxidative stress related responses to achieve a better understanding of the importance of the cellular redox balance as a modulator in gamma radiation stress. A. thaliana performs like a rather radioresistant plant species as no alterations on growth and only minor alterations in the nutrient profile were observed. Gamma irradiation did not seem to induce an NADPH mediated oxidative burst and lipid peroxidation appeared to be directly induced by ionizing radiation rather than mediated through LOX activity. As ionizing radiation can cause indirect damage via water radiolysis, H2O2 is hypothesized to be an important reactive oxygen species under radiation stress. Although most H2O2-scavenging enzymes remained unchanged, important alterations were observed for CAT1, CAT2 and CAT3expression.

Type
Research Article
Information
Radioprotection , Volume 46 , Issue 6: ICRER 2011 – International Conference on Radioecology & Environmental Radioactivity: Environment & Nuclear Renaissance , 2011 , pp. S401 - S407
Copyright
© Owned by the authors, published by EDP Sciences, 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Daly K. and Thompson K.H., Int. J. Radiat. Biol. 28 (1975) 61-66.
Kovalchuk O., Kovalchuk I., Titov V., Arkhipov A. and Hohn B.,Mutat. Res. 446 (1999) 49-55.
Zaka R., Chenal C. and Misset M.T., Sci. Total Environ. 320(2004) 121-129.
Kovalchuk I., Molinier J., Yao Y., Arkhipov A. and Kovalchuk O., Mutat. Res. 624 (2007) 101-113.
Sax K., Am. J. Bot. 42 (1954) 360-364.
Sparrow A.H. and Miksche J.P., Science 134 (1961) 282-283.
Arkhipov N.P., Kuchma N.D., Askbrant S., Pasternak P.S. and Musica V.V., Sci. Total Environ. 157 (1994) 383-386.
Wi S.G., Chung B.Y., Kim J.H., Baek M.H., Yang D.H., Lee J.W. and Kim J.S., J. Plant Biol. 48 (2005) 195-200.
PROTECT Deliverable 5 (2008).
Ward J.F., Prog. Nucleic Acid Re. 35 (1988) 95-125.
Mittler R., Vanderauwera S., Gollery M. and Van Breusegem F., Trends Plant Sci. 9 (2004) 490-498.
Wada H., Koshiba T., Matsui T. and Satô M., Plant Sci. 132 (1998) 109-119.
Zaka R., Vandecasteele C.M. and Misset M.T., J. Exp. Bot. 376 (2002) 1979-1987.
Kim J.H., Chung B.Y., Kim J.S. and Wi S.G.,J. Plant Biol. 48 (2005) 47-56.
Nagata T., Yamada H., Du Z., Todoriki S. and Kikuchi S., J. Agric. Food Chem. 53 (2005) 1022-1030.
Dhindsa R.S., Plumb-Dhindsa P. and Thorpe T.A., J. Exp. Bot. 32 (1981) 93-101.
Bergmeyer H.U., Gawehn K. and Grassl, M., in Methods of Enzymatic Analysis (Academic Press, New York, 1974) pp. 425-522.
Imberty A., Goldberg R. and Catesson A.M., Plant Sci. Lett. 35 (1984) 103-108.
Gerbling K.P., Kelly G.J., Fischer K.H. and Latzko E., J. Plant Physiol. 115 (1984) 59-67.
McCord J.M. and Fridovich, I., J. Biol. Chem. 244 (1969) 6049-6055.
Remans T., Smeets K., Opdenakker K., Mathijsen D., Vangronsveld J. and Cuypers A., Planta 227 (2008) 1343-1349.
Vandenhove H., Vanhoudt N., Cuypers A., Van Hees M., Wannijn J and Horemans N., Plant Physiol. Biochem. 48 (2010) 778-786.
ERICA, Deliverable 5 (2006).
Foyer C.H., Descouvrières P. and Kunert K.J., Plant Cell Environ. 17 (1994) 507-523.
Agrawal S.B. and Mishra S., Ecotox. Environ. Safe. 72 (2009) 610-618.
Porta H. and Rocha-Sosa M., Plant Physiol. 130 (2002) 15-21.