Skip to main content
×
Home
    • Aa
    • Aa

Neuronal BC1 RNA structure: Evolutionary conversion of a tRNAAla domain into an extended stem-loop structure

  • TIMOFEY S. ROZHDESTVENSKY (a1) (a2), ALEXEI M. KOPYLOV (a2), JÜRGEN BROSIUS (a1) and ALEXANDER HÜTTENHOFER (a1)
    • Published online: 04 May 2001
Abstract

By chemical and enzymatic probing, we have analyzed the secondary structure of rodent BC1 RNA, a small brain-specific non-messenger RNA. BC1 RNA is specifically transported into dendrites of neuronal cells, where it is proposed to play a role in regulation of translation near synapses. In this study we demonstrate that the 5′ domain of BC1 RNA, derived from tRNAAla, does not fold into the predicted canonical tRNA cloverleaf structure. We present evidence that by changing bases within the tRNAAla domain during the course of evolution, an extended stem-loop structure has been created in BC1 RNA. The new structural domain might function, in part, as a putative binding site for protein(s) involved in dendritic transport of BC1 RNA within neurons. Furthermore, BC1 RNA contains, in addition to the extended stem-loop structure, an internal poly(A)-rich region that is supposedly single stranded, followed by a second smaller stem-loop structure at the 3′ end of the RNA. The three distinct structural domains reflect evolutionary legacies of BC1 RNA.

Copyright
Corresponding author
Reprint requests to: Jürgen Brosius, Institute of Experimental Pathology/Molecular Neurobiology, Von-Esmarch-Str. 56, 48149 Münster, Germany; e-mail: RNA.world@uni-muenster.de.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

RNA
  • ISSN: 1355-8382
  • EISSN: 1469-9001
  • URL: /core/journals/rna
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 64 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 22nd May 2017. This data will be updated every 24 hours.

Errata