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Poly(A)-binding proteins regulate both mRNA deadenylation and decapping in yeast cytoplasmic extracts

Published online by Cambridge University Press:  11 January 2002

CAROL J. WILUSZ ,
MIN GAO ,
CHARLES L. JONES ,
JEFFREY WILUSZ  and
STUART W. PELTZ
CAROL J. WILUSZ
Affiliation:
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey– Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
MIN GAO
Affiliation:
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey– New Jersey Medical School, Newark, New Jersey 07103, USA
CHARLES L. JONES
Affiliation:
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey– Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
JEFFREY WILUSZ
Affiliation:
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey– New Jersey Medical School, Newark, New Jersey 07103, USA
STUART W. PELTZ
Affiliation:
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey– Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
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Abstract

The pathway of mRNA degradation has been extensively studied in the yeast, Saccharomyces cerevisiae, and it is now clear that many mRNAs decay by a deadenylation-dependent mechanism. Although several of the factors required for mRNA decay have been identified, the regulation and precise roles of many of the proteins involved remains unclear. We have developed an in vitro system that recapitulates both the deadenylation and the decapping steps of mRNA decay. Furthermore, both deadenylation and decapping are inhibited by poly(A) binding proteins in our assay. Our system has allowed us to separate the decay process from translation and we have shown that the poly(A) tail is capable of inhibiting decapping in an eIF4E-independent manner. Our in vitro system should prove invaluable in dissecting the mechanisms of mRNA turnover.

Keywords

eIF4EmRNA decaypoly(A) tailtranslation
Type
Research Article
Information
RNA , Volume 7 , Issue 10 , October 2001 , pp. 1416 - 1424
Copyright
2001 RNA Society

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