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Problems with the transorientation hypothesis

  • SCOTT M. STAGG (a1), MIKEL VALLE (a2), RAJENDRA K. AGRAWAL (a3) (a4), JOACHIM FRANK (a2) (a3) and STEPHEN C. HARVEY (a1)...
Abstract

A novel mechanism for the very first step in polypeptide elongation on the ribosome has recently been proposed by Simonson and Lake (2002). According to their transorientation hypothesis, the incoming aminoacyl-transfer RNA (aa-tRNA) initially binds to the messenger RNA (mRNA) with the anticodon stacked on the 5′ side of the anticodon loop; then, following GTP hydrolysis, the tRNA swings into the A-site by switching from the 5′-stacked conformation to the classic 3′-stacked geometry while maintaining Watson–Crick base pairing with the mRNA. There are several serious problems with this proposal.

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Copyright
Corresponding author
Reprint requests to: Stephen C. Harvey, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA; e-mail: harvey@uab.edu.
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RNA
  • ISSN: 1355-8382
  • EISSN: 1469-9001
  • URL: /core/journals/rna
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