Skip to main content
×
×
Home

Splicing and transcription-associated proteins PSF and p54nrb/NonO bind to the RNA polymerase II CTD

  • ANDREW EMILI (a1) (a2), MICHAEL SHALES (a1), SUSAN McCRACKEN (a1), WEIJUN XIE (a3), PHILIP W. TUCKER (a3), RYUJI KOBAYASHI (a4) (a5), BENJAMIN J. BLENCOWE (a1) (a2) and C. JAMES INGLES (a1) (a2)...
Abstract

The carboxyl-terminal domain (CTD) of the largest subunit of eukaryotic RNA polymerase II (pol II) plays an important role in promoting steps of pre-mRNA processing. To identify proteins in human cells that bind to the CTD and that could mediate its functions in pre-mRNA processing, we used the mouse CTD expressed in bacterial cells in affinity chromatography experiments. Two proteins present in HeLa cell extract, the splicing and transcription-associated factors, PSF and p54nrb/NonO, bound specifically and could be purified to virtual homogeneity by chromatography on immobilized CTD matrices. Both hypo- and hyperphosphorylated CTD matrices bound these proteins with similar selectivity. PSF and p54nrb/NonO also copurified with a holoenzyme form of pol II containing hypophosphorylated CTD and could be coimmunoprecipitated with antibodies specific for this and the hyperphosphorylated form of pol II. That PSF and p54nrb/NonO promoted the binding of RNA to immobilized CTD matrices suggested these proteins can interact with the CTD and RNA simultaneously. PSF and p54nrb/NonO may therefore provide a direct physical link between the pol II CTD and pre-mRNA processing components, at both the initiation and elongation phases of transcription.

Copyright
Corresponding author
Reprint requests to: C. James Ingles, Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada; e-mail: cj.ingles@utoronto.ca
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

RNA
  • ISSN: 1355-8382
  • EISSN: 1469-9001
  • URL: /core/journals/rna
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed