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Non-deep complex morphophysiological dormancy in Narcissus longispathus (Amaryllidaceae): implications for evolution of dormancy levels within section Pseudonarcissi

Published online by Cambridge University Press:  01 March 2013

José M. Herranz
Affiliation:
Unit of Ecology and Botany, Escuela Técnica Superior de Ingenieros Agrónomos, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, Spain
Elena Copete
Affiliation:
Unit of Ecology and Botany, Escuela Técnica Superior de Ingenieros Agrónomos, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, Spain
Pablo Ferrandis*
Affiliation:
Unit of Ecology and Botany, Escuela Técnica Superior de Ingenieros Agrónomos, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, Spain
*
*Correspondence Fax: +34 967 599238 E-mail: pablo.ferrandis@uclm.es

Abstract

Narcissus longispathus (Amaryllidaceae) is a perennial geophyte and a Mediterranean narrowly endemic species. At dispersal time, N. longispathus seeds are dormant and have underdeveloped embryos. This work aimed to determine requirements for dormancy break and germination and to compare dormancy traits with those of the two endemic Iberian congeners. Phenology of embryo growth and germination were studied by regularly exhuming seeds sown in near-natural conditions. Temperature and light requirements for embryo growth, breaking of dormancy and germination were determined by incubating seeds under controlled laboratory conditions. Mean embryo length in fresh seeds was 1.50 mm, and embryos had to grow to 3.80 mm before radicle emergence. Embryos grew to full size and seeds germinated when they were warm stratified for 2 months (optimum 1 month at 20/7°C+1 month at 15/4°C), then cold stratified at 5°C for 2 months, and finally incubated at cool temperatures (15/4°C) for 30 d. However, in seeds only subjected to either warm or cold stratification, the embryos hardly grew and did not germinate. In natural conditions, the embryos elongate in autumn–winter, and in late winter–early spring (March) almost all radicles and seedlings emerged. Velocity of embryo growth and germination percentages increased with seed storage duration. Seeds of N. longispathus have non-deep complex morphophysiological dormancy (MPD). This is the first report of such a level of MPD in Narcissus. Our data suggest that non-deep complex MPD may have been derived from intermediate complex MPD in the section Pseudonarcissi.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2013 

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References

Aizpuru, I., Ballester, G., Bañares, A., Báscones, J.C., Benito, J.L., Blanca, G., Blanché, C., Blanco, E., Bramwell, D., Bueno, A., et al, (2000) Lista Roja de Flora Vascular Española. Conservación Vegetal 6, 1138.Google Scholar
Bañares, A., Blanca, G., Güemes, J., Moreno, J.C.andOrtiz, S. (Eds) (2003) Atlas y Libro Rojo de la Flora Vascular Amenazada de España. Madrid, Dirección General de Conservación de la Naturaleza.Google Scholar
Barret, S.C.H., Cole, W.W. and Herrera, C.M. (2004) Mating patterns and genetic diversity in the wild daffodil Narcissus longispathus (Amaryllidaceae). Heredity 92, 459465.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1984) Germination ecophysiology of the woodland herb Osmorhiza longistylis (Umbelliferae). American Journal of Botany 71, 687692.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1985) Seed germination ecophysiology of the woodland spring geophytes Erythronium albidum. Botanical Gazette 146, 130136.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1991) Nondeep complex morphophysiological dormancy in seeds of Osmorhiza claytonii (Apiaceae). American Journal of Botany 78, 588593.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1994) Deep complex morphophysiological dormancy in seeds of the mesic woodland herb Delphinium tricorne (Ranunculaceae). International Journal of Plant Sciences 155, 738743.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. San Diego, California, Academic Press.Google Scholar
Baskin, C.C., Chester, E.W. and Baskin, J.M. (1992) Deep complex morphophysiological dormancy in seeds of Thaspium pinnatifidum (Apiaceae). International Journal of Plant Sciences 153, 565571.CrossRefGoogle Scholar
Baskin, C.C., Milberg, P., Andersson, L. and Baskin, J.M. (2000) Deep complex morphophysiological dormancy in seeds of Anthriscus sylvestris (Apiaceae). Flora 195, 245251.CrossRefGoogle Scholar
Beckstead, J., Meyer, S.E. and Allen, P.S. (1996) Bromus tectorum seed germination: between-population and between-year variation. Canadian Journal of Botany 74, 875882.CrossRefGoogle Scholar
Blanchard, J. (1990) Narcissus, a guide to wild daffodils. Woking, UK, Alpine Garden Society.Google Scholar
Cerabolini, B., De Andrés, R., Ceriani, R.M., Pierce, S. and Raimondi, B. (2004) Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosa and Primula glaucescens. Biological Conservation 117, 351356.CrossRefGoogle Scholar
Copete, M.A., Herranz, J.M. and Ferrandis, P. (2005) Seed dormancy and germination in threatened Iberian Coincya (Brassicaceae) taxa. Ecoscience 12, 257266.CrossRefGoogle Scholar
Copete, E., Herranz, J.M., Ferrandis, P., Baskin, C.C. and Baskin, J.M. (2011a) Physiology, morphology and phenology of seed dormancy-break and germination in the endemic Iberian species Narcissus hispanicus (Amaryllidaceae). Annals of Botany 107, 10031016.CrossRefGoogle ScholarPubMed
Copete, E., Herranz, J.M., Copete, M.A., Baskin, J.M. and Baskin, C.C. (2011b) Nondeep complex morphophysiological dormancy in seeds of the Iberian Peninsula endemic geophyte Merendera montana (Colchicaceae). Seed Science Research 21, 267281.CrossRefGoogle Scholar
Dorda, E. and Fernández-Casas, J. (1994) Estudios morfológicos en el género Narcissus L. Anatomía de hojas y escapo, IV. Fontquería 39, 69150.Google Scholar
Feder, M.E., Bennett, A.F. and Huey, R.B. (2000) Evolutionary physiology. Annual Review of Ecology and Systematics 31, 315341.CrossRefGoogle Scholar
Fernandes, A. (1975) L'evolution chez le genre Narcissus L. Anales Instituto Botánico de Cavanilles 32, 843872.Google Scholar
Forbis, T.A. and Diggle, P.K. (2001) Subnivean embryo development in the alpine herb Caltha leptosepala (Ranunculaceae). Canadian Journal of Botany 79, 635642.CrossRefGoogle Scholar
Giménez-Benavides, L., Escudero, A. and Pérez-García, F. (2005) Seed germination of high mountain Mediterranean species: altitudinal, interpopulation and interannual variability. Ecological Research 20, 433444.CrossRefGoogle Scholar
Graham, S.W. and Barret, S.C.H. (2004) Phylogenetic reconstruction of the evolution of stylar polymorphisms in Narcissus (Amaryllidaceae). American Journal of Botany 91, 10071021.CrossRefGoogle ScholarPubMed
Hernández-Bermejo, E., García, M.A., Contreras, P. and Clemente, M. (1999) Narcissus nevadensis Pugsley. pp. 195198in Blanca, G.; Cabezudo, B.; Hernández-Bermejo, E.; Herrera, C.M.; Molero Mesa, J.; Muñoz, J.; Valdés, B. (Eds) Libro rojo de la flora silvestre amenazada de Andalucía. Especies en peligro de extinción. Sevilla, Consejería de Medio Ambiente, Junta de Andalucía.Google Scholar
Herranz, J.M., Copete, M.A., Ferrandis, P. and Copete, E. (2010a) Intermediate complex morphophysiological dormancy in the endemic iberic Aconitum napellus subsp. castellanum (Ranunculaceae). Seed Science Research 20, 109121.CrossRefGoogle Scholar
Herranz, J.M., Ferrandis, P. and Martínez-Duro, E. (2010b) Seed germination ecology of the threatened endemic Iberian Delphinium fissum subsp. sordidum (Ranunculaceae). Plant Ecology 211, 89106.CrossRefGoogle Scholar
Herranz, J.M., Copete, M.A. and Ferrandis, P. (2013) Environmental regulation of embryo growth, dormancy break and germination of Narcissus alcaracensis (Amaryllidaceae), a threatened daffodil endemic iberic. American Midland Naturalist 169, 147167.CrossRefGoogle Scholar
Herrera, C.M., Hernández-Bermejo, E., Luque, P. and Benavente, A. (1999) Narcissus longispathus Pugsley. pp. 191194in Blanca, G.; Cabezudo, B.; Hernández-Bermejo, E.; Herrera, C.M.; Molero Mesa, J.; Muñoz, J.; Valdés, B. (Eds) Libro Rojo de la flora silvestre amenazada de Andalucía. Especies en peligro de extinción. Sevilla, Consejería de Medio Ambiente, Junta de Andalucía.Google Scholar
Hidayati, S.N., Baskin, J.M. and Baskin, C.C. (2000) Dormancy-breaking and germination requirements of seeds of four Lonicera species (Caprifoliaceae) with underdeveloped embryos. Seed Science Research 10, 459469.CrossRefGoogle Scholar
Hidayati, S.N., Baskin, J.M. and Baskin, C.C. (2001) Dormancy-breaking and germination requirements for seeds of Symphoricarpos orbiculatus (Caprifoliaceae). American Journal of Botany 88, 14441451.CrossRefGoogle ScholarPubMed
Kondo, T., Miura, T., Okubo, N., Shimada, M., Baskin, C.C. and Baskin, J.M. (2004) Ecophysiology of deep simple epicotyl morphophysiological dormancy in seeds of Gagea lutea (Liliaceae). Seed Science Research 14, 371378.CrossRefGoogle Scholar
Luna, B., Pérez, B., Fernández-González, F. and Moreno, J.M. (2004) Sensitivity to green safelight of 12 Mediterranean species. Seed Science and Technology 32, 113117.CrossRefGoogle Scholar
Mathew, B. (2002) Classification of the genus Narcissus. pp. 3052in Hanks, G.R. (Ed.) Narcissus and daffodil: the genus Narcissus. New York, Taylor and Francis.Google Scholar
Medail, F. and Quezel, P. (1997) Hot-spots analysis for conservation of plant biodiversity in the Mediterranean basin. Annals Missouri Botanical Garden 84, 112127.CrossRefGoogle Scholar
Medrano, M. and Herrera, C.M. (2008) Geographical structuring of genetic diversity across the whole distribution range of Narcissus longispathus, a habitat-specialist, Mediterranean narrow endemic. Annals of Botany 102, 183194.CrossRefGoogle ScholarPubMed
Meyer, S.E., McArthur, E.D. and Jorgensen, G.L. (1989) Variation in germination response to temperature in rubber rabbitbrush (Chrysothamnus nauseosus: Asteraceae) and its ecological implications. American Journal of Botany 76, 981991.CrossRefGoogle Scholar
Moreno, J.C.(coord.) (2008) Lista Roja 2008 de la Flora Vascular Española. Madrid, Dirección General del Medio Natural y Política Forestal, Ministerio de Medio Ambiente y Medio Rural y Marino.Google Scholar
Moreno, J.C. and Sainz, H. (1992) Atlas corológico de las monocotiledóneas endémicas de la Península Ibérica y Baleares. Madrid, Colección Técnica, ICONA.Google Scholar
Navarro, L. and Guitián, J. (2003) Seed germination and seedling survival of two threatened endemic species of the northwest Iberian Peninsula. Biological Conservation 109, 313320.CrossRefGoogle Scholar
Nikolaeva, M.G. (1977) Factors controlling the seed dormancy pattern. pp. 5174in Khan, A.A. (Ed.) The physiology and biochemistry of seed dormancy and germination. Amsterdam, North-Holland.Google Scholar
Phartyal, S.S., Kondo, T., Baskin, J.M. and Baskin, C.C. (2009) Temperature requirements differ for the two stages of seed dormancy break in Aegopodium podagraria (Apiaceae), a species with deep complex morphophysiological dormancy. American Journal of Botany 96, 10861095.CrossRefGoogle ScholarPubMed
Ríos-Ruiz, S., Rivera-Nunez, D., Alcaraz-Ariza, F. and Obon-de-Castro, C. (1999) Three new species of Narcissus L. subgenus ajax Spach (Amaryllidaceae), restricted to the meadows and forests of south-eastern Spain. Botanical Journal of the Linnean Society 131, 153165.CrossRefGoogle Scholar
Scholten, M., Donahue, J., Shaw, N.L. and Serpe, M.D. (2009) Environmental regulation of dormancy loss in seeds of Lomatium dissectum (Apiaceae). Annals of Botany 103, 10911101.CrossRefGoogle ScholarPubMed
Stokes, P. (1953) The stimulation of growth by low temperature in embryos of Heracleum sphondylium L. Journal of Experimental Botany 4, 222234.CrossRefGoogle Scholar
Threadgill, P.F., Baskin, J.M. and Baskin, C.C. (1981) Dormancy in seeds of Frasera caroliniensis. American Journal of Botany 68, 8086.CrossRefGoogle Scholar
Valle, F., Gómez, F., Mota, J.F. and Díaz de la Guardia, C. (1989) Parque Natural de Cazorla, Segura y las Villas. Guía botánico-ecológica. Alcorcón, Madrid, Ed. Rueda.Google Scholar
Vandelook, F. and Van Assche, J.A. (2008a) Temperature requirements for seed germination and seedling development determine timing of seedling emergence of three monocotyledonous temperate forest spring geophytes. Annals of Botany 102, 865875.CrossRefGoogle ScholarPubMed
Vandelook, F. and Van Assche, J.A. (2008b) Deep complex morphophysiological dormancy in Sanicula europaea (Apiaceae) fits a recurring pattern of dormancy types in genera with an Arcto-Tertiary distribution. Botany 86, 13701377.CrossRefGoogle Scholar
Vandelook, F., Bolle, N. and Van Assche, J.A. (2007) Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-atlantic genus. Annals of Botany 100, 233239.CrossRefGoogle ScholarPubMed
Walck, J.L. and Hidayati, S.N. (2004) Germination ecophysiology of the western North American species Osmorhiza depauperata (Apiaceae): implications of preadaptation and phylogenetic niche conservation in seed dormancy evolution. Seed Science Research 14, 387394.CrossRefGoogle Scholar
Walck, J.L., Hidayati, S.N. and Okagami, N. (2002) Seed germination ecophysiology of the Asian species Osmorhiza aristata (Apiaceae): comparison with its North American congeners and implications for evolution of types of dormancy. American Journal of Botany 89, 829835.CrossRefGoogle ScholarPubMed
Zonneveld, B.J.M. (2008) The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae). Plant Systematics and Evolution 275, 109132.CrossRefGoogle Scholar