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Differences in seed germination response of two populations of Phelipanche ramosa (L.) Pomel to a set of GR24 concentrations and durations of stimulation

Published online by Cambridge University Press:  15 September 2021

Stéphanie Gibot-Leclerc Open the ORCID record for Stéphanie Gibot-Leclerc [Opens in a new window] ,
Manon Connault ,
Rémi Perronne Open the ORCID record for Rémi Perronne [Opens in a new window]  and
Fabrice Dessaint Open the ORCID record for Fabrice Dessaint [Opens in a new window]
Stéphanie Gibot-Leclerc*
Affiliation:
Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne, Univ. Bourgogne-Franche-Comté, Dijon F-21000, France
Manon Connault
Affiliation:
Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne, Univ. Bourgogne-Franche-Comté, Dijon F-21000, France
Rémi Perronne
Affiliation:
IGEPP, INRAE, Institut Agro, Univ. de Rennes, Le Rheu F-35653, France
Fabrice Dessaint
Affiliation:
Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne, Univ. Bourgogne-Franche-Comté, Dijon F-21000, France
*
Author for Correspondence: Stéphanie Gibot-Leclerc, E-mail: stephanie.gibot-leclerc@inrae.fr
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Abstract

Phelipanche ramosa is a major weed holoparasite characterized by a broad host range with a suboptimal development on numerous hosts, suggesting inter- or intra-species specificities. Seeds of P. ramosa germinate after exposure to exogenous chemicals exuded by surrounding host roots such as strigolactones, the concentrations of these germination stimulants varying between hosts. In France, P. ramosa is characterized by genetically differentiated populations presenting varying germination rates and a host specificity. The objective of our study was to investigate the sensitivity of seeds of two P. ramosa populations harvested on tobacco and oilseed rape, to a set of GR24 concentrations, a synthetic strigol analogue. The assessment of the germination rate was based on in vitro experiments. Seeds of P. ramosa were placed in Petri dishes with various concentrations of GR24. The cumulative number of germinated seeds of P. ramosa was counted several times after application of the treatment. Cumulative germination curves were analysed using a three-parameter log-logistic model and a time-to-event approach. The results show that the germination rate of P. ramosa seeds depends on the GR24 concentration and the duration of stimulation, but also that the response to these two factors varies greatly according to the origin of the P. ramosa seeds. The difference in germination speed between P. ramosa populations further shows distinct responses at the intraspecific level, thus suggesting that the specialization of P. ramosa probably occurs at least from the first stage of the holoparasite cycle.

Keywords

arable weeddose-responseoilseed rapeparasitic plantstrigolactonetime-to-event approachtobacco
Type
Short Communication
Information
Seed Science Research , Volume 31 , Issue 3 , September 2021 , pp. 243 - 248
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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