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Differential RNA- and protein-expression profiles of cactus seeds capable of hydration memory

Published online by Cambridge University Press:  09 October 2013

Eduardo López-Urrutia ,
Martha Martínez-García ,
Alejandro Monsalvo-Reyes ,
Victor Salazar-Rojas ,
Raymundo Montoya  and
Jorge E. Campos
Eduardo López-Urrutia
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
Martha Martínez-García
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
Alejandro Monsalvo-Reyes
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
Victor Salazar-Rojas
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
Raymundo Montoya
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
Jorge E. Campos*
Affiliation:
Unidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No 1, Los Reyes Iztacala, Tlalnepantla, Estado de México Apdo Postal 314 C.P. 54000, México
*
*Correspondence Email: jcampos@unam.mx
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Abstract

Hydration memory is a phenomenon in which a seed can tolerate discontinuous hydration periods, displaying enhanced germination after one or multiple hydration–dehydration (HD) cycles; it was described physiologically in a few cactus species around 15 years ago. Although no additional work was done on this subject, it has great biotechnological potential since its analysis would permit predictions about whether a seed can withstand discontinuous hydration; in the long run, the knowledge about its regulation might lead to induction of this resistance, so we aimed to provide an initial approach to the molecular mechanisms that underlie hydration memory. This phenomenon was reproduced successfully in our lab with Ferocactus peninsulae seeds. Using two-dimensional (2D) electrophoresis, we compared expression patterns of proteins involved in seed maturation of seeds and seedlings subjected to an HD cycle treatment. We found differential expression of several proteins possibly involved in primary metabolism, ubiquitination pathway and reserve protein availability regulation in seeds and seedlings subjected to an HD cycle. We also found differential stability of total RNA. These results strongly suggest that the differential expression of proteins is at least partially related to the hydration memory process.

Keywords

2D PAGEFerocactus peninsulaegene expressiongerminationhydration memory

Information

Type
Research Papers
Information
Seed Science Research , Volume 24 , Issue 2 , June 2014 , pp. 91 - 99
Copyright
Copyright © Cambridge University Press 2013 

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