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Dormancy in white-grain mutants of Chinese Spring wheat (Triticum aestivum L.)

  • R.L Warner (a1), D.A. Kudrna (a1), S.C. Spaeth (a1) and S.S. Jones (a1)
Abstract
Abstract

Red wheats (Triticum aestivum L.) are generally more dormant and sprout resistant than white wheats. Whether this is caused by pleiotropic effectsof the red grain colour genes (R) on dormancy and coat colour, or to tight linkage between R and dormancy genes has not been fully resolved. To directly determine the effect of the R1 allele on dormancy, mutations were induced with sodium azide in a pure line selection of the red genotype (R1R1r2r2r3r3) Chinese Spring wheat. Two white mutants (CSW01, CSW02) were recovered from M3 caryopses derived from approximately 20,000 M2 plants. Both mutants were shown to be allelic to a domesticwhite genotype (r1r1r2r2r3r3). Except for seed coat colour, CSW01 and CSW02 are morphologically indistinguishable from the wild type and are presumed to be near isogenic lines of Chinese Spring. Freshly harvested grainsproduced under four different environments were evaluated for post-harvest dormancy. In all environments, intact caryopses of all three isolines exhibited high temperature dormancy typical of cereal species, although the red wild type consistently exhibited greater dormancy than the white mutant isolines. Dormancy was dissipated by afterripening in dry storage at 37°C in a similar manner for the red and white isolines. Excised embryos of the three isolines exhibited similar levels of dormancy and sensitivities to exogenous abscisic acid. These results indicate a functional R1 allele is not absolutely required for dormancy in wheat, but does enhance its expression in caryopses with dormant (sensitive) embryos

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*Fax: (509) 335-8674 Email: rwarner@wsu.edu
References
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Bewley J.D. (1997) Seed germination and dormancy. Plant Cell 9, 10551066.
Bhatt G.M. and Derera N.F. (1980) Potential use of Kenya 321-type dormancy in a wheat breeding programme aimed atevolving varieties tolerant to preharvest sprouting. Cereal Research Communications 8, 291295.
Black M., Butler J. and Hughes M. (1987) Control and development of dormancy in cereals. pp. 379392 in Mares D.J. (Ed) Fourth international symposium on preharvest sprouting in cereals. Boulder, Westview Press.
Bradbury D., MacMasters M.M. and Cull I.M. (1956) Structure of the mature wheat kernel. II. Microscopic structure of pericarp, seed coat, and other coverings of the endosperm andgerm of hard red winter wheat. Cereal Chemistry 33, 342360.
Buraas T. and Skinnes H. (1984) Genetic investigations on seed dormancy in barley. Hereditas 101, 235244.
Chmelar F. and Mostovoj K. (1938) On theapplication of some old and on the introduction of new methods for testing genuineness of variety in the laboratory. Proceedings of the International Seed Testing Association 10, 6874.
Chopra S., Athma P. and Peterson T. (1996) Alleles of the maize P gene with distinct tissue specificities encode myb-homologous proteins with C-terminal replacements. Plant Cell 8, 11491158.
DePauw R.M. and McCaig T.N. (1983) Recombining dormancy from RL4137 with white seed color. pp. 251259 in Kruger J.E., LaBerge D.E. (Eds) Third international symposium on pre-harvest sprouting in cereals. Boulder, Westview Press.
DePauw R.M. and McCaig T.N. (1990) Advances in the development of sprouting resistant white wheats. pp. 241247 in Ringlund K., Mosleth E., Mares D.J. (Eds) Fifth international symposium on pre-harvest sprouting in cereals. Boulder, Westview Press.
Foley M.E. and Fennimore S.A. (1998) Genetic basis for seed dormancy. Seed Science Research 8, 173182.
Freed R.D., Everson E.H., Ringlund K. and Gullord M. (1976) Seed coat colour in wheat and the relationship to seed dormancy at maturity. Cereal Research Communications 4, 147149.
Gale M.D. (1989) The genetics of preharvest sproutingin cereals, particularly in wheat. pp. 85110 in Derera N.F. (Ed) Preharvest field sprouting in cereals. Boca Raton, CRC Press, Inc.
George D.W. (1967) High temperature seed dormancyin wheat (Triticum aestivum L.). Crop Science 7, 249253.
Gfeller F. and Svejda F. (1960) Inheritance of post-harvest seed dormancy and kernel colour in spring wheat lines. Canadian Journal of Plant Science 40, 16.
Grotewold E., Drummond B.J., Bowen B. and Peterson T. (1994) The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell 76, 543553.
Hilhorst H.W.M. (1995) A critical update on seed dormancy. I. Primary dormancy. Seed Science Research 5, 6173.
Kleinhofs A., Warner R.L., Muehlbauer F.J. and Nilan R.A. (1978) Induction and selection of specific gene mutations in Hordeum and Pisum. Mutation Research 51, 2935.
MacMasters M.M., Hinton J.J.C. and Bradbury D. (1971) Microscopic structure and composition of the wheat kernel. pp. 51113 in Pomeranz Y. (Ed) Wheat chemistry and technology. St. Paul, American Association of Cereal Chemists.
Mares D.J. (1983) Preservation of dormancy in freshly harvested wheat grain. Australian Journal of Agricultural Research 34, 3338.
Mares D.J. (1987) Pre-harvest sprouting tolerance in white grained wheat. pp. 6474 in Mares D.J. (Ed) Fourth International symposium on pre-harvest sprouting in cereals. Boulder, Westview Press.
Mares D.J. (1993) Genetic studies of sprouting tolerance in red and white wheats. pp. 2129 in Walker-Simmons M.K., Ried J.L. (Eds) Pre-harvest sprouting in cereals 1992. St. Paul, MN, American Association of Cereal Chemists.
Mares D.J. (1996) Dormancy in white wheat: mechanism and location of genes. pp. 179184 in Noda K., Mares D.J. (Eds) Seventh international symposium on pre-harvest sprouting in cereals 1995. Osaka, Center for Academic Societies Japan.
Mares D.J. and Ellison F.W. (1990) Dormancy and preharvest sprouting tolerance in white-grained and redgrained wheats. pp. 7584 in Ringlund K., Mosleth E., Mares D.J. (Eds) Fifth international symposium on preharvest sprouting in cereals. Boulder, Westview Press.
McCrate A.J., Nielsen M.T., Paulsen G.M. and Heyne E.G. (1982) Relationship between sprouting in wheat and embryo response to endogenous inhibition. Euphytica 31, 193200.
McEwan J.M. (1976) Relative sprouting resistance of closely-related wheats differing in grain colour. Cereal Research Communications 4, 151155.
McIntosh R.A. (1987) Gene location and gene mapping in hexaploid wheat. pp. 269287 in Heyne E.G. (Ed) Wheat and wheat improvement. Madison, American Society ofAgronomy.
Miyamoto T. and Everson E.H. (1958) Biochemical and physiological studies of wheat seed pigmentation. Agronomy Journal 50, 733734.
Miyamoto T., Tolbert N.E. and Everson E.H. (1961) Germination inhibitors related to dormancy in wheat seeds. Plant Physiology 36, 739746.
Morris C.F., Anderberg R.J., Goldmark P.J. and Walker-Simmons M.K. (1991) Molecular cloning and expression of abscisic acid-responsive genes in embryos of dormant wheat seeds. Plant Physiology 95, 814821.
Morris C.F., Moffatt J.M., Sears R.G. and Paulsen G.M. (1989) Seed dormancy and responses of caryopses, embryos, and calli to abscisic acid in wheat. Plant Physiology 90, 643647.
Morris C.F., Mueller D.D., Faubion J.M. and Paulsen G.M. (1988) Identification of L-tryptophan as an endogenous inhibitor of embryo germination in white wheat. Plant Physiology 88, 435440.
Stoy V. and Olsen O.A. (1980) Inheritance of a factor affecting the response to germination inhibitors in excisedwheat embryo. Cereal Research Communications 8, 203208.
Stoy V. and Sundin K. (1976) Effects of growth regulating substances in cereal seed germination. Cereal Research Communications 4, 157163.
Styles E.D. and Ceska O. (1989) Pericarpflavonoids in genetic strains of Zea mays. Maydica 34, 227237.
Walker-Simmons M. (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiology 84, 6166.
Walker-Simmons M. (1988) Enhancement of ABA responsiveness in wheat embryos by high temperature. Plant, Celland Environment 11, 769775
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Seed Science Research
  • ISSN: 0960-2585
  • EISSN: 1475-2735
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