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Dynamics of the processes leading to the acquisition of sensitivity to very low fluence of photons in Datura ferox seeds

Published online by Cambridge University Press:  30 August 2013

Gabriela Alejandra Auge  and
Lucila de Miguel
Gabriela Alejandra Auge*
Affiliation:
IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Av. San Martín 4453, (1417)Ciudad Autónoma de Buenos Aires, Argentina
Lucila de Miguel
Affiliation:
IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Av. San Martín 4453, (1417)Ciudad Autónoma de Buenos Aires, Argentina
*
*Correspondence Email: gabriela.auge@duke.edu
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Abstract

Soil tillage operations stimulate germination of buried seeds in cultivated lands, allowing them to perceive light as a germination-promoting factor. The time of burial and the effect of changing environmental factors affect the physiological state of the seeds, which may lead to an extreme light-sensitivity and very low fluence response (VLFR) through phytochrome A. This paper describes the influence of the progressive process of dormancy breakage, which is accompanied by the acquisition of extreme light-sensitivity, on processes associated with endosperm weakening and embryo growth potential in the VLFR-mediated promotion of Datura ferox seed germination. Our results show that endosperm weakening is mainly limited by β-mannosidase enzyme activity after far-red light stimulation, which is highly dependent on the dormancy level of the seeds. In addition, stimulation of the embryo growth potential by far-red irradiation did not require an extreme light-sensitivity to very low fluence of photons to reach its maximum response, and it was not completely correlated with expansin gene expression in the embryo. Our work indicates that responses of endosperm weakening and embryo growth potential to far-red irradiation, dependent on dormancy level, have different requirements for stimulation by the signalling network initiated by phytochrome A during the course of the very low fluence response in Datura ferox seeds.

Keywords

Datura feroxembryo growth potentialendosperm weakeninglow fluence responsephytochrome Avery low fluence response
Type
Research Papers
Information
Seed Science Research , Volume 23 , Issue 4 , December 2013 , pp. 233 - 239
Copyright
Copyright © Cambridge University Press 2013 

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