Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-24T00:05:06.290Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentum Mill.) seeds

Published online by Cambridge University Press:  19 September 2008

Yongqing Liu ,
R. J. Bino ,
W. J. van der Burg ,
S. P. C. Groot  and
H. W. M. Hilhorst
Yongqing Liu
Affiliation:
Department of Horticulture, Hunan Agricultural University, Changsha 410128, China
R. J. Bino*
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
W. J. van der Burg
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
S. P. C. Groot
Affiliation:
Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
H. W. M. Hilhorst
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
*
*Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Freshly harvested tomato (Lycopersicon esculentum Mill, cv. Moneymaker) seeds were osmotically primed for 8 d in −1.0 MPa PEG-6000 solution and dried to about 6% water content for storage. Such so–called ‘fresh PEG priming’ enhanced seed germination and improved seedling performance as compared with the untreated control. Fresh PEG priming neither alleviated seed dormancy nor promoted DNA replication as was the case when seeds were dried upon harvest and subsequently primed in PEG (normal PEG priming). However, the addition of 10 μM GA4+7 to the osmotic priming solution triggered replicative DNA synthesis of fresh-priming seeds and further enhanced the germination process. After 5 months of storage in ambient temperature conditions, fresh PEG-primed seeds maintained more positive effects gained from priming, whereas, normal PEG-primed seeds had lost the promoting effects on germination. Normal PEG-primed seeds were much more susceptible to controlled deterioration than fresh PEG-primed seeds. It is suggested that the advancement of germination is negatively correlated with seed storability. The mechanisms of seed priming in relation to nuclear replication activities and physical changes are discussed.

Keywords

DNA replicationdormancyfree spacefresh primingtomato seed
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 6 , Issue 2 , June 1996 , pp. 49 - 55
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdul-Baki, A.A. and Stoner, A. (1978) Germination promoter and inhibitor in leachates from tomato seed. Journal of the American Society for Horticultural Science 103, 684–686.CrossRefGoogle Scholar
Argerich, C.A. and Bradford, K.J. (1989a) The effects of priming and ageing on seed vigour in tomato. Journal of Experimental Botany 40, 599–607.CrossRefGoogle Scholar
Argerich, C.A. and Bradford, K.J. (1989b) The effects of priming and ageing on the resistance to deterioration of tomato seeds. Journal of Experimental Botany 40, 593–598.CrossRefGoogle Scholar
Basu, R.N. and Pal, P. (1979) Physico-chemical control of seed deterioration in rice. Indian Journal of Agricultural Science 49, 1–6.Google Scholar
Bewley, J.D. and Black, M. (1985) Seeds. Physiology of development and germination. pp 175–233. New York and London, Plenum Press.CrossRefGoogle Scholar
Bino, R.J., De Vries, J.N., Kraak, H.L. and Van Pijlen, J.G. (1992) Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Annals of Botany 69, 231–236.CrossRefGoogle Scholar
Bino, R.J., Lanteri, S., Verhoeven, H.A. and Kraak, H.L. (1993) Flow cytometric determination of nuclear replication stages in seed tissues. Annals of Botany 72, 181–187.CrossRefGoogle Scholar
Demir, I. and Ellis, R.H. (1992) Changes in seed quality during seed development and maturation in tomato. Seed Science Research 2, 81–87.CrossRefGoogle Scholar
Finch-Savage, W.E. and McQuistan, C. (1991) Abscisic acid: an agent to advance and synchronize germination for tomato (Lycopersicon esculentum Mill) seeds. Seed Science and Technology 19, 537–544.Google Scholar
Finch-Savage, W.E., Gray, D. and Dickson, G.M. (1991) The combined effects of osmotic priming with plant growth regulator and fungicide soaks on the seed quality of five bedding plant species. Seed Science and Technology 19, 495–503.Google Scholar
Georghiou, K., Thanos, C.A. and Passam, H.C. (1987) Osmoconditioning as means of counteracting the ageing of pepper seeds during high temperature storage. Annals of Botany 66, 279–285.CrossRefGoogle Scholar
Groot, S.P.C. (1987) Hormonal regulation of seed development and germination in tomato. Ph.D. Thesis, Agricultural University, Wageningen, Netherlands.Google Scholar
Groot, S.P.C. and Karssen, C.M. (1987) Gibberellins regulate seed germination in tomato by endosperm weakening: a study with gibberellin-deficient mutants. Planta 171, 525–531.CrossRefGoogle ScholarPubMed
Groot, S.P.C. and Karssen, C.M. (1992) Dormancy and germination of abscisic acid-deficient tomato seeds. Studies with the sitiens mutant. Plant Physiology 99, 952–958.CrossRefGoogle ScholarPubMed
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance and attempted prognosis. Seed Science and Technology 5, 952–958.Google Scholar
Heydecker, W., Higgins, J. and Gulliver, R.L. (1973) Accelerated germination by osmotic seed treatment. Nature 236, 42–14.CrossRefGoogle Scholar
Hilhorst, H.W.M. (1995) A critical update on seed dormancy. I. Primary dormancy. Seed Science Research 5, 61–73.CrossRefGoogle Scholar
ISTA (1993) International rules for seed testing. Rules 1993. Seed Science and Technology 21, Supplement.Google Scholar
Jacobsen, J.V. and Chandler, P.M. (1987) Gibberellin and abscisic acid in germinating cereals, pp 164 in Davies, P.J. (Ed.) Plant hormones and their role in plant growth and development. Dordrecht, Martinus Nijhoff Publishers.Google Scholar
Karssen, C.M., Zagorski, S., Kepczynski, J. and Groot, S.P.C. (1989) Key role for endogenous-gibberellins in the control of seed germination. Annals of Botany 63, 71–80.CrossRefGoogle Scholar
Khan, A.A., Tao, K.L., Knypl, J.S., Borkowska, B. and Powell, L.E. (1978) Osmotic conditioning of seeds: physiological and biochemical changes. Acta Horticulturae 83, 267–278.CrossRefGoogle Scholar
Lanteri, S., Saracco, F., Kraak, H.L. and Bino, R.J. (1994) The effects of priming on nuclear replication activity and germination of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds. Seed Science Research 4, 81–87.CrossRefGoogle Scholar
Liptay, A. and Zariffa, N. (1993) Testing the morphological aspects of polyethylene glycol-primed tomato seeds with proportion odds analysis. HortScience 28, 881–883.CrossRefGoogle Scholar
Liu, Y., Van der Burg, W.J., Aartse, J.W., Van Zwol, R.A., Jalink, H. and Bino, R.J. (1993) X-ray studies on changes in embryo and endosperm morphology during priming and imbibition of tomato seeds. Seed Science Research 3, 171–178.CrossRefGoogle Scholar
Liu, Y., Bergervoet, J.H.W., Ric, De, Vos, C.H., Hilhorst, H.W.M., Kraak, H.L., Karssen, C.M. and Bino, R.J. (1994) Nuclear replication activities during imbibition of abscisic acid- and gibberellin-deficient tomato (Lycopersicon esculentum Mill.) seeds. Planta 194, 368–373.CrossRefGoogle Scholar
Michel, B.E. and Kaufmann, M.R. (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology 51, 914–916.CrossRefGoogle ScholarPubMed
Odland, M.L. (1938) Observations on dormancy in vegetable seeds. Proceedings of the American Society for Horticultural Science 35, 562–565.Google Scholar
Osborne, D.J. (1983) Biochemical control systems operating in the early hours of germination. Canadian Journal of Botany 61, 3568–3577.CrossRefGoogle Scholar
Penaloza, A.P.S. and Eira, M.T.S. (1993) Hydration-dehydration treatments on tomato seeds (Lycopersicon esculentum Mill). Seed Science and Technology 21, 309–316.Google Scholar
Rao, N.K., Roberts, E.H. and Ellis, R.H. (1987) The influence of pre- and post-storage hydration treatments on chromosomal aberrations, seedling abnormalities, and viability of lettuce seeds. Annals of Botany, 60, 97–108.CrossRefGoogle Scholar
Tarquis, A. and Bradford, K.J. (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seed. Journal of Experimental Botany 43, 307–317.CrossRefGoogle Scholar
Weges, R. (1987) Physiological analysis of methods to relieve dormancy of lettuce seeds. Ph.D. Thesis, Agricultural University, Wageningen, Netherlands.Google Scholar