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The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

  • Ruth C. Martin (a1), Masashi Asahina (a2), Po-Pu Liu (a2), Jessica R. Kristof (a2), Jennifer L. Coppersmith (a2), Wioletta E. Pluskota (a2), George W. Bassel (a2), Natalya A. Goloviznina (a2), Theresa T. Nguyen (a2), Cristina Martínez-Andújar (a2), M.B. Arun Kumar (a2), Piotr Pupel (a2) and Hiroyuki Nonogaki (a2)...

MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156⊣SPL13 → miR172⊣SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.

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Seed Science Research
  • ISSN: 0960-2585
  • EISSN: 1475-2735
  • URL: /core/journals/seed-science-research
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