Skip to main content Accessibility help

Evaluation of mechanical properties of fixed bone cells with sub-micrometer thickness by picosecond ultrasonics

  • Mathieu Ducousso (a1) (a2), Omar El-Farouk Zouani (a3), Christel Chanseau (a3), Céline Chollet (a3), Clément Rossignol (a1) (a2), Bertrand Audoin (a1) (a2) and Marie-Christine Durrieu (a3)...

The picosecond ultrasonics technique is used to investigate the viscoelastic properties of nucleus of fixed single osteoblast progenitor cells adhering on a titanium alloy substrate. A two-color probing picosecond ultrasonics and a fluorescence visualization setups were developed and combined to allow to distinguish subcomponents inside the cell under investigation. It opens the way for quantitative measurements of the viscoelastic properties of single cells and of their sub-micrometer thickness. It is shown that a blue probe, λ = 400 nm, is preferable to a red probe, λ = 800 nm, to perform these measurements with fixed sub-micrometer bone cells. 26 GHz acoustic frequencies are detected in cells as thin as 135 nm. A 1D analytical model of the acoustic generation and of the optical detection is used to describe the experimental results. The nucleus longitudinal elastic moduli (13–16 GPa) and dynamic viscosities (13–30 cP) are measured at high frequencies (GHz) from a time-frequency analysis of the experimental data of fixed single cells.

Corresponding author
Hide All
[1]Cross, S.E., Jin, Y.-S., Rao, J., Gimzewski, J.K., Nature Nanotechnol. 2, 780 (2007)
[2]Anselme, K., Biomater. 21, 667 (2000)
[3]Rotsch, C., Radmacher, M., Biophys. J. 78, 520 (2000)
[4]Van Vliet, K.J., Bao, G., Suresh, S., Acta Mater. 51, 5881 (2003)
[5]Lim, C.T., Zhou, E.H., Li, A., Vedula, S.R.K., Fu, H.X., Mat. Sci. Eng.: C 26, 1278 (2006)
[6]Gennisson, J.L., Baldeweck, T., Tanter, M., Catheline, S., Fink, M., Sandrin, L., Cornillon, C., Querleux, B., IEEE Trans. Ultrason. Ferroelectr. Freq. Control 51, 980 (2004)
[7]Wells, P.N.T., Liang, H.D., J. R. Soc. Interface 8, 1521 (2011)
[8]Bereiter-Hahn, J., Blase, C., Ultrasonic Characterization of Biological Cells (CRC Press, Boca Raton, FL, 2003)
[9]Lemor, R.M., Weiss, E., Zinin, P., Pilarczyk, G., IEEE Int. Ultrason. Symp. Proc. 1, 622 (2004)
[10]Karl, I., Bereiter-Hahn, J., Cell Biochem. Biophys. 29, 225 (1998)
[11]Thomsen, C., Strait, J., Vardeny, Z., Maris, H.J., Tauc, J., Hauser, J.J., Phys. Rev. Lett. 53, 989 (1984)
[12]Antonelli, G.A., Perrin, B., Daly, B.C., Cahill, D.G., MRS Bull. 31, 607 (2006)
[13]Scherbakov, A.V., Salasyuk, A.S., Akimov, A.V., Liu, X., Bombeck, M., Brüggemann, C., Yakovlev, D.R., Sapega, V.F., Furdyna, J.K., Bayer, M., Phys. Rev. Lett. 105, 117204 (2010)
[14]Thevenard, L., Peronne, E., Gourdon, C., Testelin, C., Cubukcu, M., Charron, E., Vincent, S., Lemaître, A., Perrin, B., Phys. Rev. B 82, 104422 (2010)
[15]Yang, F., Atay, T., Dang, C.H., Grimsley, T.J., Che, S., Ma, J., Zhang, Q., Nurmikko, A.V., Maris, H.J., J. Phys.: Conf. Ser. 92, 012024 (2007)
[16]Pezeril, T., Klieber, C., Andrieu, S., Nelson, K.A., Phys. Rev. Lett. 102, 107402 (2009)
[17]Decremps, F., Belliard, L., Perrin, B., Gauthier, M., Phys. Rev. Lett. 100, 035502 (2008)
[18]Rossignol, C., Chigarev, N., Ducousso, M., Audoin, B., Forget, G., Guillemot, F., Durrieu, M.C., Appl. Phys. Lett. 93, 123901 (2008)
[19]Audoin, B., Rossignol, C., Chigarev, N., Ducousso, M., Forget, G., Guillemot, F., Durrieu, M.C., Ultrasonics 50, 202 (2010)
[20]Ducousso, M., Dehoux, T., Rossignol, C., Audoin, B., Zouani, O., Chollet, C., Chanseau, C., Durrieu, M.C., J. Phys.: Conf. Ser. 278, 012042 (2011)
[21]Audoin, B., Ducousso, M., Dehoux, T., Chollet, C., Zouani, O., Chanseau, C., Durrieu, M.-C., Photons Plus Ultrasound: Imaging and Sensing 78990X (2011)
[22]Perrin, B., Microscale and nanoscale heat transfer (Springer, Berlin, 2007)
[23]Verdier, C., Etienne, J., Duperray, A., Preziosi, L., C. R. Phys. 10, 790 (2009)
[24]Starodubtseva, M.N., Ageing Res. Rev. 10, 16 (2011)
[25]Rouch, J., Lai, C.C., Chen, S.H., J. Chem. Phys. 65, 4016 (1976)
[26]Yang, F., Grimsley, T.J., Che, S., Antonelli, G.A., Maris, H.J., Nurmikko, A.V., J. Appl. Phys. 107, 103537 (2010)
[27]Auld, B.A., Acoustic Fields and Waves in Solids, vol. 1 (Robert E. Krieger Publishing Company, 1990)
[28]Thomsen, C., Grahn, H.T., Maris, H.J., Tauc, J., Phys. Rev. B 34, 4129 (1986)
[29]Devos, A., Robillard, J.F., Côte, R., Emery, P., Phys. Rev. B 74, 064114 (2006)
[30]Kashiwada, S., Matsuda, O., Baumberg, J.J., Li Voti, R., Wright, O.B., J. Appl. Phys. 100, 073506 (2006)
[31]Ducousso, M., Dehoux, T., Audoin, B., Zouani, O., Chollet, C., Durrieu, M.C., J. Phys.: Conf. Ser. 269, 012024 (2011)
[32]Auld, B.A., Acoustic Fields and Waves in Solids (Wiley, New York 1973)
[33]Ducousso, M., Dehoux, T., Audoin, B., Zouani, O., Chollet, C., Durrieu, M.-C., J. Phys.: Conf. Ser. 269, 012024 (2010)
[34]Rossignol, C., Perrin, B., Laborde, S., Vandenbulcke, L., Barros, M.I.D., Djemia, P., J. Appl. Phys. 95, 4157 (2004)
[35]Wright, O.B., J. Appl. Phys. 71, 1617 (1992)
[36]Dehoux, T., Chigarev, N., Rossignol, C.M., Audoin, B., Phys. Rev. B 76, 024311 (2007)
[37]Wright, O., Perrin, B., Matsuda, O., Gusev, V.E., Phys. Rev. B 78, 024303 (2008)
[38]Dil, J.G., Rep. Prog. Phys. 45, 285 (1982)
[39]Born, M., Wolf, E., Principles of Optics (Cambridge University Press, 1998)
[40]Houser, G.D., Garmire, E., Appl. Opt. 33, 1059 (1994)
[41]Werley, C.A., Teo, S.M., Nelson, K.A., Rev. Sci. Instrum. 82, 123108 (2011)
[42]Long, M., Rack, H.J., Biomater. 19, 1621 (1998)
[43]Zouani, O.F., Chollet, C., Guillotin, B., Durrieu, M.-C., Biomater. 31, 8245 (2010)
[44]Beuthan, J., Minet, O., Helfmann, J., Herrig, M., Müller, G., Phys. Med. Biol. 41, 369 (1996)
[45]Hatton, J.P., Pooran, M., Li, C.F., Luzzio, C., Hughes-Fulford, M., J. Bone Miner. Res. 18, 58 (2003)
[46]Caille, N., Thoumine, O., Tardy, Y., Meister, J.-J., J. Biomech. 35, 177 (2002)
[47]Pontecorvo, E., Ortolani, M., Polli, D., Ferretti, M., Ruocco, G., Cerullo, G., Scopigno, T., Appl. Phys. Lett. 98, 011901 (2011)
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The European Physical Journal - Applied Physics
  • ISSN: 1286-0042
  • EISSN: 1286-0050
  • URL: /core/journals/the-european-physical-journal-applied-physics
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed