Hostname: page-component-848d4c4894-wzw2p Total loading time: 0 Render date: 2024-06-10T03:55:46.506Z Has data issue: false hasContentIssue false

Tomographic diffractive microscopy of transparent samples

Published online by Cambridge University Press:  16 April 2008

B. Simon
Affiliation:
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
M. Debailleul
Affiliation:
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
V. Georges
Affiliation:
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
V. Lauer
Affiliation:
Lauer Microscopie, 1bis rue des Blés, 68200 Mulhouse, France
O. Haeberlé*
Affiliation:
Laboratoire MIPS-LabEl, IUT Mulhouse, 61 rue A. Camus 68093 Mulhouse Cedex, France
Get access

Abstract

We report a tomographic diffractive microscope, which permits imaging non-labelled transparent or semi-transparent samples. Based on a combination of microholography with a tomographic illumination, our set-up creates 3-D images of the index of refraction distribution within the sample. One acquires successively interferograms, rotating the illumination (the specimen being static) and using phase-shifting holography. Within the first Born approximation, each interferogram is interpreted as a subset of the Fourier transform of the specimen index of refraction distribution. The reconstruction is therefore similar to synthetic aperture imaging: one recombines the information in the Fourier space, and a final Fourier transform gives a 3-D image of the specimen. First recalling the theoretical foundations, we then describe our experiment, and show initial results obtained on biological samples.

Keywords

Type
Research Article
Copyright
© EDP Sciences, 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Wolf, E., Opt. Commun. 1, 153 (1969) CrossRef
Charrière, F. et al., Opt. Lett. 31, 178 (2006) CrossRef
Fauver, M. et al., Opt. Exp. 13, 4210 (2005) CrossRef
M. Born, E. Wolf, Principles of Optics (Cambridge University Press, Cambridge, 1999)
Alexandrov, S.A. et al., Phys. Rev. Lett. 97, 168102 (2006) CrossRef
Guo, P., Devaney, A.J., J. Opt. Soc. Am. A 22, 2338 (2005) CrossRef
Belkebir, K., Chaumet, P.C., Sentenac, A., J. Opt. Soc. Am. A 23, 586 (2006) CrossRef
Chaumet, P.C., Belkebir, K., Sentenac, A., Opt. Lett. 29, 2740 (2004) CrossRef
Kawata, S., Nakamura, O., Minami, S., J. Opt. Soc. Am. A 4, 292 (1987) CrossRef
Lauer, V., J. Microsc. 205, 165 (2002) CrossRef
Gorski, W., Osten, W., Opt. Lett. 32, 1977 (2007) CrossRef
Noda, T., Kawata, S., Minami, S., Appl. Opt. 31, 670 (1992) CrossRef
W. Jueptner, U. Schnars, Digital Holography Digital Hologram Recording, Numerical Reconstruction, and Related Techniques (Springer, Berlin, 2005)
Hell, S.W., Stelzer, E.H.K., Opt. Commun. 93, 277 (1992) CrossRef
Gustafsson, M.G.L., Agard, D.A., Sedat, J.W., J. Microsc. 195, 10 (1999) CrossRef
M.L. Laucks et al., J. Aerosol Sci. 31, 307 (2000)
Pappas, C.S. et al., Appl. Spectr. 57, 23 (2003) CrossRef