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Color discrimination ellipses of trichromats measured with transient and steady state visual evoked potentials

Published online by Cambridge University Press:  03 July 2008

BRUNO D. GOMES ,
GIVAGO S. SOUZA ,
MONICA G. LIMA ,
ANDERSON R. RODRIGUES ,
CÉZAR A. SAITO ,
MANOEL DA SILVA FILHO  and
LUIZ CARLOS L. SILVEIRA
BRUNO D. GOMES*
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
GIVAGO S. SOUZA
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
MONICA G. LIMA
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
ANDERSON R. RODRIGUES
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
CÉZAR A. SAITO
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
MANOEL DA SILVA FILHO
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil
LUIZ CARLOS L. SILVEIRA
Affiliation:
Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil Núcleo de Medicina Tropical, Universidade Federal do Pará, Brazil
*
Address correspondence and reprint requests to: Bruno Duarte Gomes, Universidade Federal do Pará, Núcleo de Medicina Tropical, Av. Generalíssimo Deodoro 92, 66055-240 Belém-Pará, Brazil. E-mail: brunodgomes@yahoo.com.br
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Abstract

The purpose of this work is to investigate the use of different forms of visual evoked potentials (VEPs) to measure color discrimination thresholds and to plot color discrimination ellipses (MacAdam, 1942). Five normal trichromats (24.5 ± 2.6 years-old) were monocularly tested. Stimuli consisted of sinusoidal isoluminant chromatic gratings made from chromaticity pairs located along four different color directions radiating from one reference point of the CIE 1976 chromaticity diagram (u′ = 0.225; v′ = 0.415). Heterochromatic flicker photometry (HFP) was used to obtain the isoluminance condition for every subject and for all chromaticity pairs. VEPs were elicited using two cycles per degree grating stimuli at three different temporal configurations: transient, onset (300 ms)/offset (700 ms), 1 Hz fundamental frequency; steady-state, onset (50 ms)/offset (50 ms), 10 Hz fundamental frequency; and steady-state pattern reversal at 5 Hz fundamental frequency (10 Hz phase reversal). VEP amplitude was measured using transient VEP N1-P1 components and steady state VEP first (10 Hz) and second (20 Hz) harmonics. VEP amplitude was plotted as a function of chromatic distance in the CIE 1976 color space and the data points were extrapolated to zero amplitude level to obtain chromatic discrimination thresholds. The results were compared with psychophysical measurements performed using the same stimulus configurations and with the pseudoisochromatic method of Mollon-Reffin (one-way ANOVA). For all subjects and all stimulation methods, the ellipses showed small sizes, low ellipticities, and were vertically oriented. Despite some consistent differences in the results obtained with different procedures, there was no statistical difference between ellipses obtained electrophysiologically and psychophysically. For steady state VEPs, ellipses obtained from second harmonic amplitudes were larger and more elongated in the tritan direction than those obtained with first harmonic amplitudes.

Keywords

Transient visual evoked potentialSteady-state visual evoked potentialMollon-Reffin methodChromatic difference thresholdsMacAdam ellipses.
Type
Research Article
Information
Visual Neuroscience , Volume 25 , Issue 3 , May 2008 , pp. 333 - 339
Copyright
Copyright © Cambridge University Press 2008

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