Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-29T09:44:02.078Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of adenosine uptake and endogenous adenosine-containing cells in mammalian retina

Published online by Cambridge University Press:  02 June 2009

Christine Blazynski ,
Judith L. Mosinger  and
Adolph I. Cohen
Christine Blazynski
Affiliation:
Department of Ophthalmology, Washington University School of Medicine, St. Louis Department of Anatomy-Neurobiology, Washington University School of Medicine, St. Louis
Judith L. Mosinger
Affiliation:
Department of Psychiatry, Washington University School of Medicine, St. Louis
Adolph I. Cohen
Affiliation:
Department of Anatomy-Neurobiology, Washington University School of Medicine, St. Louis
Get access Rights & Permissions [Opens in a new window]

Abstract

Autoradiographic techniques were used to label [3H]-adenosine and [3H]-cyclohexyladenosine accumulating cells in rabbit, mouse, and ground squirrel retinas. Immunohistochemical methods revealed the distribution of cells that stained for endogenous adenosine. Comparisons of these two markers revealed for all three species that the distribution of specific subpopulations of retinal cells that store or accumulate the purine nucleoside, adenosine, is similar. For all three species, cells localized in the ganglion cell layer accumulated adenosine and exhibited adenosine-like immunoreactivity (ALIR). A smaller proportion of cells localized in the inner nuclear layer were labeled for ALIR, while a larger proportion of cells in this layer accumulated adenosine. Subtle differences between species are presented. However, the general similarities of the distribution of these two putative purinergic markers supports the evidence that a discrete adenosinergic neurotransmitter/modulatory system is present in the retina.

Keywords

AdenosineMammalian retinaPurines
Type
Research Articles
Information
Visual Neuroscience , Volume 2 , Issue 2 , February 1989 , pp. 109 - 116
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blazynski, C. (1987). Adenosine A1 receptor-mediated inhibition of adenylate cyclase in rabbit retina. Journal of Neuroscience 7, 2522–2528.Google ScholarPubMed
Blazynski, C., Kinscherf, D.A., Geary, K.M. & Ferrendelli, J.A. (1985). Adenosine-mediated regulation of cyclic AMP levels in isolated, incubated retinas. Brain Research 366, 224–229.CrossRefGoogle Scholar
Braas, K.M., Newby, A.C., Wilson, V.S. & Snyder, S.H. (1986). Adenosine-containing neurons in the brain localized by immunocytochemistry. Journal of Neuroscience 6, 1952–1961.CrossRefGoogle ScholarPubMed
Braas, K.M., Zarbin, M.A. & Snyder, S.H. (1987). Endogenous adenosine and adenosine receptors localized to ganglion cells of the retina. Proceedings of the National Academy of Sciences of the U.S.A. 84, 3906–3910.CrossRefGoogle ScholarPubMed
Cammer, W., Sacchi, R. & Kahn, S. (1985). Immunocytochemical localization of 5′-nucleotidase in oligodendroglia and myelinated fibers in the central nervous system of adult and young rats. Developmental Brain Research 20, 89–96.CrossRefGoogle Scholar
Cohen, A.I., McDaniel, M. & Orr, H. (1973). Absolute levels of some free amino acids in normal and biologically fractionated retinas. Investigative Ophthalmology and Visual Science 12, 689–693.Google Scholar
Ehinger, B. & Perez, M.T.R. (1984). Autoradiography of nucleoside uptake into the retina. Neurochemistry International 6, 369–381.CrossRefGoogle ScholarPubMed
Geiger, J.D., LaBella, F.S. & Nagy, J.I. (1985). Characterization of nitrobenzylthioinosine binding to nucleoside transport sites selective for adenosine in rat brain. Journal of Neuroscience 5, 735–740.Google Scholar
Geiger, J.D. & Nagy, J.I. (1984). Heterogeneous distribution of adenosine transport sites labeled by [3H]-nitrobenzylthioinosine in rat brain: an autoradiographic and membrane binding study. Brain Research Bulletin 13, 657–666.CrossRefGoogle Scholar
Goodman, R.R., Kuhar, M.J., Hester, L. & Snyder, S.H. (1983). Adenosine receptors: autoradiographic evidence for their location of axon terminals of excitatory neurons. Science 220, 967–969.Google Scholar
Goodman, R.R. & Snyder, S.H. (1982). Autoradiographic localization of adenosine receptors in rat brain using [3H]-cyclohexyladenosine. Journal of Neuroscience 2, 1230–1241.Google Scholar
Hsu, S.-M., Raine, L. & Fanger, H. (1981). Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. Journal of Histochemistry and Cytochemistry 29, 577–580.Google Scholar
Kreutzberg, G.W. & Hussain, S.T. (1982). Cytochemical heterogeneity of the glial plasma membrane: 5′-nucleotidase in retinal Müller cells. Journal of Neurocytology 11, 53–64.CrossRefGoogle ScholarPubMed
Marangos, P.J. (1984). Differentiating adenosine receptors and adenosine uptake sites in brain. Journal of Receptor Research 4, 231–244.CrossRefGoogle ScholarPubMed
Mosinger, J.L., Yazulla, S. & Studholme, K.M. (1986). GABA-like immunoreactivity in the vertebrate retina: a species comparison. Experimental Eye Research 42, 631–644.CrossRefGoogle ScholarPubMed
Nagy, J.I., LaBella, L.A. & Buss, M. (1984). Immunohistochemistry of adenosine deaminase: implications for adenosine neurotransmission. Science 224, 166–168.Google Scholar
Newby, A. & Sala, G.B. (1982). A new procedure for haptenizing adenosine leading to a more specific radioimmunoassay method. Biochemical Journal 208, 603–610.CrossRefGoogle ScholarPubMed
Paes de Carvalho, R. & De Mello, F.G. (1982). Adenosine-elicited accumulation of adenosine 3′,5′-cyclic monophosphate in the chick embryo retina. Journal of Neurochemistry 38, 493–500.Google Scholar
Paes de Carvalho, R. & De Mello, F.G. (1985). Expression of AI adenosine receptors modulating dopamine-dependent cyclic AMP accumulation in the chick embryo retina. Journal of Neurochemistry 44, 845–851.Google Scholar
Perez, M.T.R. & Brunn, A. (1987). Colocalization of [3H]-adenosine accumulation and GABA immunoreactivity in the chicken and rabbit retinas. Histochemistry 87, 413–417.CrossRefGoogle ScholarPubMed
Perez, M.T.R., Ehinger, B.E., Lindstrom, K. & Fredholm, B.B. (1986). Release of endogenous and radioactive purines from the rabbit retina. Brain Research 398, 106–112.Google Scholar
Phillis, J.W. & Barraco, R.A. (1985). Adenosine, adenylate cyclase, and transmitter release. Advances in Cyclic Nucleotide and Protein Phosphorylation Research 19, 243–257.Google Scholar
Phillis, J.W. & Wu, P.H. (1981). The role of adenosine and its nucleotides in central synaptic transmission. Advances in Cyclic Nucleotide and Protein Phosphorylation Research 19, 187–239.Google Scholar
Senba, E., Daddona, P.E. & Nagy, J.I. (1986). Immunohistochemical localization of adenosine deaminase in the retina of the rat. Brain Research Bulletin 17, 209–217.CrossRefGoogle ScholarPubMed
Trussell, L.O. & Jackson, M.B. (1985). Adenosine-activated potassium conductance in cultured striatal neurons. Proceedings of the National Academy of Sciences of the U.S.A. 82, 4857–4861.Google Scholar
Trussell, L.O. & Jackson, M.B. (1987). Dependence of an adenosine-activated potassium current on a GTP-binding protein in mammalian central neurons. Journal of Neuroscience 7, 3306–3316.CrossRefGoogle ScholarPubMed
Williams, M. (1987). Purinergic receptors and central nervous system function. In Psychopharmacology: The Third Generation of Progress, ed. Meltzer, H.Y., pp. 289–301. New York: Raven Press.Google Scholar
Yazulla, S., Studholme, K. & Zucker, C. (1985). Synaptic organization of substance-P like immunoreactive amacrine cells in the goldfish retina. Journal of Comparative Neurology 231, 232–238.CrossRefGoogle ScholarPubMed