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    Zakharov, Vladislav V. and Mosevitsky, Mark I. 2010. Oligomeric structure of brain abundant proteins GAP-43 and BASP1. Journal of Structural Biology, Vol. 170, Issue. 3, p. 470.
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    Feig, Sherry L. 2004. Corticothalamic cells in layers 5 and 6 of primary and secondary sensory cortex express GAP-43 mRNA in the adult rat. The Journal of Comparative Neurology, Vol. 468, Issue. 1, p. 96.
    Kobayashi, H. Ochi, K. Saito, I. Hanada, K. and Maeda, T. 1998. Alterations in Ultrastructural Localization of Growth-associated Protein-43 (GAP-43) in Periodontal Ruffini Endings of Rat Molars during Experimental Tooth Movement. Journal of Dental Research, Vol. 77, Issue. 3, p. 503.
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    Fields, R.Douglas and Nelson, Phillip G. 1992. Vol. 34, Issue. , p. 133.
    Daw, N. W. Sato, H. Fox, K. Carmichael, T. and Gingerich, R. 1991. Cortisol reduces plasticity in the kitten visual cortex. Journal of Neurobiology, Vol. 22, Issue. 2, p. 158.
    Dani, J.W. Armstrong, D.M. and Benowitz, L.I. 1991. Mapping the development of the rat brain by GAP-43 immunocytochemistry. Neuroscience, Vol. 40, Issue. 1, p. 277.
    McIntosh, Helen and Blazynski, Christine 1991. GAP-43-like immunoreactivity in the adult retina of several species. Brain Research, Vol. 554, Issue. 1-2, p. 321.
    McIntosh, Helen and Parkinson, David 1990. GAP-43 in adult visual cortex. Brain Research, Vol. 518, Issue. 1-2, p. 324.
    McIntosh, Helen Daw, Nigel and Parkinson, David 1990. GAP-43 in the cat visual cortex during postnatal development. Visual Neuroscience, Vol. 4, Issue. 06, p. 585.
    DiFiglia, Marian Roberts, Rosalinda C. and Benowitz, Larry I. 1990. Immunoreactive GAP-43 in the neuropil of adult rat neostriatum: Localization in unmyelinated fibers, axon terminals, and dendritic spines. The Journal of Comparative Neurology, Vol. 302, Issue. 4, p. 992.
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A GAP-43-like protein in cat visual cortex

  • Helen McIntosh (a1), David Parkinson (a1), Karina Meiri (a2), Nigel Daw (a1) and Mark Willard (a2)...
Abstract

We have purified a protein that changes in relative concentration during the development of the kitten visual cortex. It resembles GAP-43 (a neuronal protein that is expressed at elevated levels during periods of development and regenerative axon growth) in the following respects: (1) it is an acidic protein (pI=4.7) whose electrophoretic mobility on SDS-PAGE is similar to, but lower than rat GAP-43, suggesting that the cat protein is larger; (2) its electrophoretic mobility varies with the acrylamide concentration in a manner that is characteristic of GAP-43; (3) its concentration in kitten forebrain is elevated during early postnatal development; (4) the sequence of ten consecutive amino acids from a chemically generated fragment matches the expected sequence from GAP-43; and (5) its amino-acid content also matches GAP-43. We conclude that our purified protein is cat GAP-43. Immunoblots with an antibody prepared against rat GAP-43 suggested that the concentration of GAP-43 in the visual cortex declines with age.

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COPYRIGHT: © Cambridge University Press 1989
References
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Akers, R.F. & Routtenberg, A. (1985). Protein kinase C phosphorylates a 47Mr protein (Fl) directly related to synaptic plasticity. Brain Research 334, 147–151.
Alexander, K.A., Cimler, B.A., Meier, K.E. & Storm, D.R. (1987). Regulation of calmodulin binding to P–57. A neurospecific cal-modulin binding protein. The Journal of Biological Chemistry 262, 6108–6113.
Basi, G.S., Jacobson, R.D., Virag, I., Schilling, J. & Skene, J.H.P. (1987). Primary structure and transcriptional regulation of GAP-43, a protein associated with nerve growth. Cell 49, 785–791.
Benowitz, L.I. & Routtenberg, A. (1987). A membrane phosphoprotein associated with neural development, axonal regeneration, phospholipid metabolism, and synaptic plasticity. Trends in Neuroscience 10, 527–532.
Benowitz, L.I. & Schmidt, J.T. (1987). Activity-dependent sharpening of the regenerating retinotectal projection in goldfish: relationship to the expression of growth-associated proteins. Brain Research 417, 118–126.
Benowitz, L.I., Perrone-Blzzozero, N.I. & Finklestein, S.P. (1987). Molecular properties of the growth-associated protein GAP-43 (B–50). Journal of Neurochemistry 48, 1640–1647.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
Cragg, B.G. (1975). The development of synapses in the visual system of the cat. Journal of Comparative Neurology 160, 147–166.
de Graan, P.N.E., Oestreicher, A.B., Schrama, L.H. & Gispen, W.H. (1986). Phosphoprotein B-50: localization and function. Progress in Brain Research 69, 37–49.
Gispen, W.H., Leunissen, J.L.M., Oestreicher, A.B., Verkleij, A.J. & Zwiers, H. (1985). Presynaptic localization of B-50 phosphoprotein: the (ACTH)-sensitive protein kinase substrate involved in rat brain polyphosphoinositide metabolism. Brain Research 328, 381–385.
Holmes, H. & Rodnight, R. (1981). Ontogeny of membrane-bound protein phosphorylating systems in the rat. Developmental Neuroscience 4, 79–88.
Hubel, D.H. & Wiesel, T.N. (1970). The period of susceptibility to the physiological effects of unilateral eye closure in kittens. Journal of Physiology (London) 195, 215–243.
Jacobson, R.D., Virag, I. & Skene, J.H.P. (1986). A protein associated with axon growth, GAP-43, is widely distributed and developmentally regulated in rat CNS. Journal of Neuroscience 6, 1843–1855.
Jones, K.R., Spear, P.D. & Tong, L. (1984). Critical periods for effects on monocular deprivation: differences between striate and extrastriate cortex. Journal of Neuroscience 4, 2543–2552.
Karns, L.R., Ng, S.-C., Freeman, J.A. & Fishman, M.C. (1987). Cloning of complementary DNA for GAP-43, a neuronal growth-related protein. Science 236, 597–600.
Kosik, K.S., Orecchio, L.D., Bruns, G.A.P., Benowitz, L.I., MacDonald, G.P., Cox, D.R. & Neve, R.L. (1988). Human GAP-43: its deduced amino-acid sequence and chromosomal localization in mouse and human. Neuron 1, 127–132.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Levay, S., Stryker, M.P. & Shatz, C.J. (1978). Ocular dominance columns and their development in layer IV of the cat's visual cortex: a quantitative study. Journal of Comparative Neurology 179, 223–244.
Lovinger, D.M. & Routtenberg, A. (1988). Synapse-specific protein kinase C activation enhances maintenance of long-term potentiation in rat hippocampus. Journal of Physiology 400, 321–333.
Masure, H.R., Alexander, K.A., Wakim, B.T. & Storm, D.R. (1986). Physicochemical and hydrodynamic characterization of P-57, a neurospecific calmodulin binding protein. Biochemistry 25, 7553–7560.
Meiri, K.F., Pfenninger, K.H. & Willard, M.B. (1986). Growth-associated protein, GAP-43, a polypeptide that is induced when neurons extend axons, is a component of growth cones and corresponds to pp46, a major polypeptide of a subcellular fraction enriched in growth cones. Proceedings of the National Academy of Sciences of the U.S.A. 83, 3537–3541.
Meyer, G. & Ferres-Torres, R. (1984). Postnatal maturation of non-pyramidal neurons in the visual cortex of the cat. Journal of Comparative Neurology 228, 226–244.
Mitchell, D.M. & Timney, B. (1984). Postnatal development of function in the mammalian visual system. In Handbook of Physiology. Section I: The Nervous System, Vol. Ill, Sensory Processes, Part I, ed. Brookhart, J.M., Mountcastle, V.B., Darian-Smith, I. & Geiger, S.R., pp 507–555. Bethesda, Maryland: American Physiological Society.
Ng, S-C., de la Monte, S.M., Conboy, G.L., Karns, L.R. & Fishman, M.C. (1988). Cloning of human GAP-43: growth association and ischemic resurgence. Neuron 1, 133–139.
O'Farrell, P.H. (1975). High-resolution two-dimensional electropho-resis of proteins. The Journal of Biological Chemistry 250, 4007–4021.
O'Farrell, P.Z., Goodman, H.M. & O'Farrell, P.H. (1977). High-resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133–1142.
Oakley, B.R., Kirsch, D.R. & Morris, N.R. (1980). A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105, 361.
Oestreicher, A.B., Van Dongen, C.J., Zwiers, H. & Gispen, W.H. (1983). Affinity-purified anti-B-50 protein antibody: interference with the function of the phosphoprotein B-50 in synaptic plasma membranes. Journal of Neurochemistry 41, 331–340.
Oestreicher, A.B., van Duin, M., Zwiers, H. & Gispen, W.H. (1984). Cross reaction of anti-rat B–50: characterization and isolation of a “B-50 phosphoprotein” from bovine brain. Journal of Neurochemistry 43, 935–943.
Olson, C.R. & Freeman, R.D. (1980). Profile of the sensitive period for monocular deprivation in kittens. Experimental Brain Research 39, 17–21.
Rosenthal, A., Chan, S.Y., Henzel, W., Haskell, C., Kuang, W.-J., Chan, E., Wilxox, J.N., Ullrich, A., Goeddel, D.V. & Routtenberg, A. (1987). Primary structure and mRNA localization of protein Fl, a growth-related protein kinase C substrate associated with synaptic plasticity. EMBO Journal 6, 3641–3646.
Shatz, C.J. & Luskin, M.B. (1986). The relationship between the geniculocortical afferents and their cortical target cells during development of the cat's primary visual cortex. Journal of Neuroscience 6, 3655–3668.
Skene, J.H.P. & Willard, M.B. (1981 a). Axonally transported proteins associated with growth in rabbit central and peripheral nervous systems. Journal of Cell Biology 89, 96–103.
Skene, J.H.P. & Willard, M.B. (1981 b). Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. Journal of Cell Biology 89, 86–95.
Skene, J.H.P., Jacobson, R.D., Snipes, G.J., McGutre, C.B., Norden, J.J. & Freeman, J.A. (1986). A protein induced during nerve growth (GAP-43) is a major component of growth-cone membranes. Science 233, 783–786.
Snipes, G.J., Chan, S.Y., McGuire, C.B., Costello, B.R., Norden, J.J., Freeman, J.A. & Routtenberg, A. (1987). Evidence for the coidentification of GAP-43, a growth-associated protein, and Fl, a plasticity-associated protein. Journal of Neuroscience 7, 4066–4075.
Warm, B.T., Alexander, K.A., Masure, H.R., Cimler, B.M., Storm, D.R. & Walsh, K.A. (1987). Amino-acid sequence of P-57, a neurospecific calmodulin-binding protein. Biochemistry 26, 7466–7470.
Winfield, D.A. (1983). The postnatal development of synapses in the different laminae of the visual cortex in the normal kitten and in kittens with eyelid suture. Developmental Brain Research 9, 155–169.
Zwiers, H., Schotman, P. & Gispen, W.H. (1980). Purification and some characteristics of an ACTH-sensitive protein kinase and its substrate protein in rat brain membranes. Journal of Neurochemis-try 34, 1689–1699.
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Visual Neuroscience
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