Skip to main content
×
Home
    • Aa
    • Aa

Regional topography of rod and immunocytochemically characterized “blue” and “green” cone photoreceptors in rabbit retina

  • E. V. Famiglietti (a1) and S. J. Sharpe (a1)
Abstract
Abstract

Evidence from several sources indicates that the photoreceptors of rabbit retina include rods, green cones and blue cones, and that blue-green color opponency occurs in select retinal ganglion cells. One of us (Famiglietti) has identified wide-field cone bipolar cells as probable blue-cone-selective bipolars, and type C horizontal cells as possibly connected to blue cones. We wished to extend the analysis of blue cone pathways in rabbit retina and to characterize the topographic distribution of blue and green cones. Two monoclonal antibodies raised against chicken visual pigments are reported to label medium- and long-wavelength cones (COS-1) and short-wavelength cones (OS-2) in all mammalian retinas studied thus far (Szél and colleagues). Using selective labeling with these two antibodies and a nonselective method in nasal and temporal halves of the same retinas, we have found that densities of photoreceptors vary systematically, depending upon the size of the eye and age of the animal. In ‘standard’ New Zealand rabbits of 2–3 kg (2–3 months old), rods reached a peak density of about 300,000/mm2 just dorsal to the visual streak, while cones exhibit peak density at mid-visual streak of about 18,000/mm2. Published measurements of visual acuity in rabbit are less than predicted by this calculation. The ratio of cones to rods is significantly higher in ventral retina, where the density of cones declines to a plateau of 10,000–12,000/mm2, when compared to dorsal retina, where cones are uniformly distributed at a density of about 7000/mm2. The density of OS-2 labeled (presumably “blue”) cones is uniformly low, 1000–1500/mm2, in a wide expanse that includes dorsal retina, the visual streak, and much of ventral retina, except for a region of higher density along the vertical midline. We confirm that there is a far ventral horizontal region near the perimeter that is populated exclusively by a high density (about 13,000/mm2) of OS-2-positive cones (Juliusson and colleagues). This region does not extend to the ventral retinal margin, however, where cone density drops precipitously. Transitional zones between COS-1 and OS-2 labeling, in a region of relatively high and uniform cone density, where sums of COS-1 and OS-2 labeling are higher than expected and in which weakly and strongly labeled cones are intermixed, raise questions about the identities of the visual pigment epitopes, the possibility of double labeling, and therefore the possibility of dual expression of pigments in single cones. The “inverted- T -shaped” topography of higher density OS-2 labeling raises doubts about the significance of a ventral concentration of blue cones for visual function in rabbit retina.

Copyright
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

J.H. Caldwell & N.W. Daw (1978). New properties of rabbit retinal ganglion cells. Journal of Physiology (London) 276, 257276.

B.G. Cleland , W.R. Levick & K.J. Sanderson (1973). Properties of sustained and transient ganglion cells in the cat retina. Journal of Physiology (London) 228, 649680.

F.M. DeMonasterio (1978). Spectral interactions in horizontal and ganglion cells of the isolated and arterially-perfused rabbit retina. Brain Research 150, 239258.

F.M. DeMonasterio , S.J. Schein & E.P. McCrane (1981). Staining of blue-sensitive cones of the macaque retina by a fluorescent dye. Science 213, 12781281.

Cugell C Enroth & J.G. Robson (1966). The contrast sensitivity of retinal ganglion cells of the cat. Journal of Physiology (London) 187, 517552.

E.V. Famiglietti (1981). Functional architecture of cone bipolar cells in mammalian retina. Vision Research 21, 15591563.

E.V. Famiglietti (1990). A new type of wide-field horizontal cell, presumably linked to blue cones, in rabbit retina. Brain Research 535, 174179.

R.M. Hill (1962). Unit responses of the rabbit lateral geniculate nucleus to monochromatic light on the retina. Science 135, 9899.

H. Höllander & J. Stone (1972). Receptor pedicle density in the cat≈s retina. Brain Research 42, 497502.

A. Hughes (1971). Topographic relationships between the anatomy and physiology of the rabbit visual system. Documenta Ophthalmologica 30, 33159.

A. Hughes (1972). A schematic eye for the rabbit. Vision Research 12, 123138.

G.H. Jacobs , J. Neitz & J.F. Deegan , III (1991). Retinal receptors in rodents maximally sensitive to ultraviolet light. Nature 353, 655656.

S.G. Jacobson , K.B.J. Franklin & W.I. McDonald (1976). Visual acuity of the cat. Vision Research 16, 11411143.

C.L. Lerea , A.H. Bunt-Milam & J.B. Hurley (1989). Alpha-transducin is present in blue-, green-, and red-sensitive cone photoreceptors in the human retina. Neuron 3, 367376.

W.R. Levick (1967). Receptive fields and trigger features of ganglion cells in the visual streak of the rabbit≈s retina. Journal of Physiology (London) 188, 285307.

R.E. Marc & H.G. Sperling (1977). Chromatic organization of primate cones. Science 196, 454456.

A.P. Mariani (1984). Bipolar cells in monkey retina selective for the cones likely to be blue-sensitive. Nature 308, 184186.

J.F.W. Nuboer (1971). Spectral discrimination in a rabbit. Documenta Ophthalmologica 30, 279298.

J.M. Provis (1979). The distribution and size of ganglion cells in the retina of the pigmented rabbit: A quantitative analysis. Journal of Comparative Neurology 185, 121138.

P. Rölich , Th van Veen , & A. Szél (1994). Two different visual pigments in one retinal cone cell. Neuron 13, 11591166.

R.H. Steinberg , M. Reid & P.L. Lacy (1973). The distribution of rods and cones in the retina of the cat (Felis domesticus). Journal of Comparative Neurology 148, 229248.

A. Szél , T. Diamantstein & P. Röhlich (1988). Identification of blue-sensitive cones in the mammalian retina by anti-visual pigment antibody. Journal of Comparative Neurology 273, 593602.

A. Szél , P. Röhlich , A.R. Caffé , B. Juliusson , G. Aguirre & T. van Veen (1992). Unique topographic separation of two spectral classes of cones in the mouse retina. Journal of Comparative Neurology 325, 327342.

A. Szél , T. van Veen & P. Röhlich (1994). Retinal cone differentiation. Nature 370, 336.

M.W. van Hof (1967). Visual acuity in the rabbit. Vision Research 7, 749751.

G.H. Walls (1942). The Vertebrate Eye and Its Adaptive Radiation. Michigan: Cranbrook Press.

H. Wässle (1971). Optical quality of the cat eye. Vision Research 11, 9951006.

H.M. Young & D.I. Vaney (1991). Rod-signal interneurons in the rabbit retina: 1. Rod bipolar cells. Journal of Comparative Neurology 310, 139153.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Visual Neuroscience
  • ISSN: 0952-5238
  • EISSN: 1469-8714
  • URL: /core/journals/visual-neuroscience
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 6 *
Loading metrics...

Abstract views

Total abstract views: 138 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 29th June 2017. This data will be updated every 24 hours.